Mageb18 (NM_173783) Mouse Tagged ORF Clone
CAT#: MR221289
- TrueORF®
Mageb18 (Myc-DDK-tagged) - Mouse melanoma antigen family B, 18 (Mageb18)
ORF Plasmid: tGFP
"NM_173783" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | B430216B18Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR221289 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGTGGCCAGAAAAGCAAACTCCGTGCCCGTGAGAAGCGCCGCCAGGCCCGTGCTAAAAATGCAC AGGAGAAAGAAGCCTCTCCAGGTCCTTTCTATGGGTGTCCACATCAGGCTAGTCCTGTTGCCAGTATGCC TACATCTCCCAACATGCCAATGGGAGAGCAGTCTACCTTTTCACACTCATATACAAGTACAAGTGACCAA AACCTAGATGAGAAAAGTTCAGATGACTCTGAGGACACTGAGGACTGGTGTAAAGATCCTATTAACCACA AAGTAGTTTTACTGGTGCAGTTCCTGATGGAGAAGTATCAAAAGAAAGAGGTTATTACTAAGGCAGATAT GCTGAAGTATGTTATCAAAACATCTAAAAACCACTTCAATGAGATCCTAAAGAGAGCCTCAGAGCACATG GAACTGGCATTTGGTATTGATCTGAAGGAAGTTGATCCAAACAGGCACTGCTATGCCCTTTTTAATAAAC TAGAACATACTTTTGATGGAGTGATGGGTGAAGAAAAAATGCCCAGTAGTGGCCTCCTAATGATTGTGCT GGGTGTGATATTCATGAATGATAACTGTGTCTCTGAAACTGAGATCTGGAATGTGCTGAGTATGATGGGT GTATATGCTAATAGAAAACACTTCATCTATGGAGATCCTAAGAAGGTCATCACTGAAGATATGGTGCAGC TAAAGTACTTGGAGTACCAACAAGTGCCCAACAGCAATCCTCCATCCTTTGAATTCACGTGGGGTCCAAG AGCCTGTGCTGAAATCAGCAAGATGAAAATCTTGGAGTTTTGGGCCAAGATCCATGATACTACACCAGAT TCCTTCCCAACCTTGTATGAAGCAGCTCTGAAAGATGAAGAAGAGAGAGCCCAAGCCAGGGCTGTAGCCA GGGCTCACACTGCTGCAATGGCCAGTCCATATTCAAGAGCCACGGTGTGCAGCTCCTCCCATACTAATAT C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221289 protein sequence
Red=Cloning site Green=Tags(s) MPRGQKSKLRAREKRRQARAKNAQEKEASPGPFYGCPHQASPVASMPTSPNMPMGEQSTFSHSYTSTSDQ NLDEKSSDDSEDTEDWCKDPINHKVVLLVQFLMEKYQKKEVITKADMLKYVIKTSKNHFNEILKRASEHM ELAFGIDLKEVDPNRHCYALFNKLEHTFDGVMGEEKMPSSGLLMIVLGVIFMNDNCVSETEIWNVLSMMG VYANRKHFIYGDPKKVITEDMVQLKYLEYQQVPNSNPPSFEFTWGPRACAEISKMKILEFWAKIHDTTPD SFPTLYEAALKDEEERAQARAVARAHTAAMASPYSRATVCSSSHTNI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_173783 |
ORF Size | 984 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_173783.3, NP_776144.1 |
RefSeq Size | 2160 bp |
RefSeq ORF | 984 bp |
Locus ID | 215641 |
UniProt ID | Q8BQR7 |
MW | 37.2 kDa |
Gene Summary | May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212624 | Mageb18 (untagged) - Mouse melanoma antigen family B, 18 (Mageb18), (10ug) |
CNY 3,990.00 |
|
MG221289 | Mageb18 (tGFP-tagged) - Mouse melanoma antigen family B 18 (Mageb18), (10ug) |
CNY 2,850.00 |
|
MR221289L3 | Lenti ORF clone of Mageb18 (Myc-DDK-tagged) - Mouse melanoma antigen family B, 18 (Mageb18) |
CNY 4,750.00 |
|
MR221289L4 | Lenti ORF clone of Mageb18 (mGFP-tagged) - Mouse melanoma antigen family B, 18 (Mageb18) |
CNY 4,750.00 |
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