Tgm7 (NM_001160424) Mouse Tagged ORF Clone

CAT#: MR221248

  • TrueORF®

Tgm7 (Myc-DDK-tagged) - Mouse transglutaminase 7 (Tgm7)

ORF Plasmid: DDK tGFP



  "NM_001160424" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,200.00

CNY 5,800.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms TGMZ; TGz
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR221248 representing NM_001160424
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCCTGTGGCAGCCTTGGAGCTCAGGTCTGTGGAACTGCAGATCCCCAGGAACAACAAGGACCACC
ACACTGAGGATTTGGGCTTGTCGAAGCTCATCCTGCGCCGTGGCCAGATCTTCATCTTAACGATAGTCTT
CAGTCGACCCTTCCAGCCCCAAAAGGATCATCTCACCTTTGTGGCTGAGACAGGACCAGAGCCCACAGAG
CTTCTGGGGACCCGAGCCACTTTCTTCCTCGCCCAGACCCGACCTGAGAACGTCTGGAGTGCTTCTGACT
TCATGATTGACAGCAACTCTCTCCAAGTCTCGATTTTCTCACCGGCTAGTGCGGTCATTGGCCACTACAC
CCTGAAGCTAGAGATCTCTCAGGGCCAGGGTCCTGGTGTGACTTACCCACTGGGGAATTTCATCTTGCTT
TTTAACCCATGGAGTACAGAGGATGATGTCTACCTGCCAAGTGAAACACTACTGCGGGAGTATATCATGA
GTGACTACGGCTTTATCTACAAGGGTCAAACAAATTCCATTACCTCCAGGCCTTGGAACTATGGGCAGTT
TGAAGAGGACATCGTCGATATCTGCTTTGAGATCCTGAACAAGAGCCTCTATTTTCTAAAGAACCCATCC
AAAGACCACTCTCAGAGAAACAACGTGGTGTATGTGTGCAGGGTGGTAAGCGCCATGATCAACAGCAATG
ACGACAGTGGTGTGCTTCAGGGGAACTGGGGAGAGGATTACTCCCAGGGCACCAGCCCTCTGGAATGGAA
CGGCAGCGTGGCCATCTTGCGGCAGTGGTCGGCCAAGGGCGGGCAGCCTGTGAAATATGGACAGTGTTGG
GTCTTTGCTTCTGTTATGTGCACGGTAATGAGATGCTTAGGTGTTCCAACCCGAGTCGTTTCCAATTTCC
ATTCTGCACACAACACAGATGGAAACTTGACCATCGACACCTACTATGATCGAACCGCAGAAATGCTGAC
GGCTCAGAGGCCAGACAAAATCTGGAACTTCCACGTCTGGAATGAGTGCTGGATGATCAGGAAAGACCTT
CCACCAGGGTACAACGGCTGGCAGGTTCTGGACCCCACCCCCCAGCAAACCAGCAGTGGGTTGTTCTGCT
GTGGCCCTGCCTCTGTGAAAGCCATCAAGGAAGGGGACGTCCAGCTGCCCTATGATACCCCTTTTGTGTA
TGCAGAAGTGAACGCTGATGAGGTCGTTTGGCTTTTTGAGGGTGGCCAGGTCCAGGAGATCCTGGCCCAT
AACACCAGTTACATTGGGAAAGCAATTAGCACCAAGATGGTAGGGTCAGATGAGCGCCTGGACATCACCA
GTTCCTACAAGTATCCAGAAGGATCCTCTGAGGAGCGGTCTGCATTCATTAAGGCTTCCTGGAAATTGCT
GGAACCGAGAAGGAACGCTTCATCTCTCCTGGATCTCCTAGGGTCTGAGAACTTAAAGGCTAAGCCTGTG
AGGCTGCAACTCCACCTGGTTAGGGCACCCATGTGGGGCCAGGACCTGCCGCTCATGCTATATGTCCAGA
GGGTGCCAGTCAGAGACCATGTTCAGGGCTCTGTCGGACTGACGGTGCGCTTTTGTGCACAGGCCCTCCT
GCATGGGGGTGGAACTCGGAAACCGTTCTGGAGACACATAGTACACTTGAATGTGGATTTTGAGAAGGAG
CTACAGTGGCCGTTCCTGCTGCCCTACAGCAGTTACAGGGACAAGCTGACAGATGAAAGGCTGATCCGTG
TGGCTGGCATCGCTGAGGTTGAAGAGACAGGGCAGTTCATTCTGGTCCTAAAAGATTTCTCTCTGGAGCC
TCCCCACTTGTCTATTGAGGTGTTTGAAAGGGCTGAGGTGGGCAAGGCTCTGGATATCCACATCACCCTC
ACCAACACCCTGATGGTGCCTCTGAATAACTGCACCATGGTTCTGGAGGGGAGTGGCCTCATCAATGGGC
AGATGACAAAAGACCTTGGGACTCTGATGGCCGGACACACTATCCGAATTCATCTGGAGCTCTACCCCAT
CAAAGCTGGGCCCCACCAGCTACAAGTCCTCATCAGCAGCAGTGAGGTCAAGGAGATCAAAGGCTACAAG
GACATCTTGGTTGCTGCAGCTCCAGCCTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR221248 representing NM_001160424
Red=Cloning site Green=Tags(s)

MDPVAALELRSVELQIPRNNKDHHTEDLGLSKLILRRGQIFILTIVFSRPFQPQKDHLTFVAETGPEPTE
LLGTRATFFLAQTRPENVWSASDFMIDSNSLQVSIFSPASAVIGHYTLKLEISQGQGPGVTYPLGNFILL
FNPWSTEDDVYLPSETLLREYIMSDYGFIYKGQTNSITSRPWNYGQFEEDIVDICFEILNKSLYFLKNPS
KDHSQRNNVVYVCRVVSAMINSNDDSGVLQGNWGEDYSQGTSPLEWNGSVAILRQWSAKGGQPVKYGQCW
VFASVMCTVMRCLGVPTRVVSNFHSAHNTDGNLTIDTYYDRTAEMLTAQRPDKIWNFHVWNECWMIRKDL
PPGYNGWQVLDPTPQQTSSGLFCCGPASVKAIKEGDVQLPYDTPFVYAEVNADEVVWLFEGGQVQEILAH
NTSYIGKAISTKMVGSDERLDITSSYKYPEGSSEERSAFIKASWKLLEPRRNASSLLDLLGSENLKAKPV
RLQLHLVRAPMWGQDLPLMLYVQRVPVRDHVQGSVGLTVRFCAQALLHGGGTRKPFWRHIVHLNVDFEKE
LQWPFLLPYSSYRDKLTDERLIRVAGIAEVEETGQFILVLKDFSLEPPHLSIEVFERAEVGKALDIHITL
TNTLMVPLNNCTMVLEGSGLINGQMTKDLGTLMAGHTIRIHLELYPIKAGPHQLQVLISSSEVKEIKGYK
DILVAAAPAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001160424
ORF Size 2130 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001160424.1, NP_001153896.1
RefSeq Size 2133 bp
RefSeq ORF 2133 bp
Locus ID 640543
MW 79.8 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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