Klf16 (NM_078477) Mouse Tagged ORF Clone

CAT#: MR221126

  • TrueORF®

Klf16 (Myc-DDK-tagged) - Mouse Kruppel-like factor 16 (Klf16)

ORF Plasmid: DDK tGFP



  "NM_078477" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,400.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AI843742; BTEB4; DRRF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR221126 representing NM_078477
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGCGGCCGTGGCGTGTGTGGATTACTTTGCCGCCGACGTGCTCATGGCCATCTCCTCGGGAGCCG
TGGTGCATCGCGGACGACCCGGCCCCGAGGGCGCGGGCCCCGCTGCCGGTCTGGATGTGCGCGCCACGCG
CCGCGAGGCCACGCCGCCGGGGACCCCTGGGGCACCGCCACCGCCAGCTACGGCTCCGGGGCCGGGAGGC
GCCACCGCTGCGCCCCACCTGCTGGCCGCCAGCATCCTGGCCGATCTGAGAGGCGGGCCGGTAGTCGCCA
CCGCAGCCAGCACCGCGGGGGGCACCTCTCCGGTCTCCTCCTCCTCAGCCGCTTCGTCTCCCTCTTCGGG
CCGCGCCCCCGGCGCCGCCAAAAGCCACCGCTGTCCCTTCCATGGCTGCGCCAAAGCCTATTACAAGTCT
TCGCACCTCAAGTCACACCTGCGGACTCACACAGGTGAGCGGCCCTTTGCCTGCGACTGGCCTGGCTGTG
ACAAGAAGTTCGCCCGTTCTGATGAGCTGGCCCGCCACCACCGGACGCACACCGGGGAGAAGCGCTTCCC
CTGCCCCCTGTGTACCAAGCGGTTCACCCGAAGCGACCACCTGACCAAGCACGCCCGGCGACATCCCGGC
TTCCGCCCGGAACTCCTGCGACGACCTGGTGCCCGCAGCGTCTCGCCCAGCGACTCGCTGCCCTGCAGCT
TGGCTGGAAGCCCCACACCTAGCCCTGTCCCCAGCCCAGCTCCTGCTGGCTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR221126 representing NM_078477
Red=Cloning site Green=Tags(s)

MSAAVACVDYFAADVLMAISSGAVVHRGRPGPEGAGPAAGLDVRATRREATPPGTPGAPPPPATAPGPGG
ATAAPHLLAASILADLRGGPVVATAASTAGGTSPVSSSSAASSPSSGRAPGAAKSHRCPFHGCAKAYYKS
SHLKSHLRTHTGERPFACDWPGCDKKFARSDELARHHRTHTGEKRFPCPLCTKRFTRSDHLTKHARRHPG
FRPELLRRPGARSVSPSDSLPCSLAGSPTPSPVPSPAPAGL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_078477
ORF Size 753 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_078477.2, NP_510962.2
RefSeq Size 2652 bp
RefSeq ORF 756 bp
Locus ID 118445
UniProt ID P58334
MW 26.1 kDa
Gene Summary Transcription factor that binds GC and GT boxes in the D1A, D2 and D3 dopamine receptor promoters and displaces Sp1 and Sp3 from these sequences. It modulates dopaminergic transmission in the brain by repressing or activating transcription from several different promoters depending on cellular context.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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