Cnot1 (NM_153164) Mouse Tagged ORF Clone
CAT#: MR221036
- TrueORF®
Cnot1 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 1 (Cnot1), transcript variant 1
ORF Plasmid: tGFP
"NM_153164" in other vectors (2)
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CNY 23,180.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 6030411K04Rik; AA815922; D830048B13 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR221036 representing NM_153164
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCTTGACTCGCTCTCGCTGGCCTTGTCTCAAATCAGCTACCTGGTGGACAATTTAACCAAGAAAA ACTACCGAGCCAGCCAGCAGGAAATACAGCATATTGTGAATCGGCACGGTCCTGAGGCAGACAGGCATTT ATTACGCTGCCTATTTTCACATGTGGATTTCAGTGGCGATGGTAAAAGCAGTGGCAAAGATTTCCATCAG ACACAGTTTCTGATTCAGGAGTGTGCCTCGCTGATTACAAAGCCAAATTTTATCTCAACATTGTCATATG CCATTGATAATCCATTGCACTACCAGAAGAGTTTAAAGCCTGCACCCCACTTATTTGCCCAGCTGAGTAA AGTTCTCAAGCTAAGTAAAGTACAGGAGGTCATTTTTGGTCTTGCCCTTTTGAATTCTTCTAGCCCAGAT CTCAGGGGTTTTGCTGCCCAGTTTATTAAACAGAAGCTTCCGGATCTTCTGCGTTCTTACATTGACGCAG ACGTCAGTGGAAATCAAGAAGGTGGCTTCCAAGATATAGCAATAGAGGTCCTACACCTCCTACTCTCCCA TCTCCTCTTTGGGCAGAAGGGAGCCTTTGGAGTTGGACAGGAACAGATAGACGCCTTTCTTAAGACACTA CGCAGAGATTTTCCCCAAGAGCGCTGTCCTGTGGTGCTTGCACCACTTTTATACCCTGAAAAACGGGACA TTCTAATGGACAGGATTTTGCCTGATTCTGGAGGGGTAGCTAAAACCATGATGGAGAGCTCGCTAGCTGA CTTCATGCAGGAAGTGGGCTATGGCTTTTGTGCAAGTATTGAAGAATGTCGCAATATAATCATGCAGTTT GGTGTTCGGGAGGTCACAGCTGCCCAGGTTGCCAGGGTTTTGGGAATGATGGCTCGGACTCATTCAGGAT TAACAGACGGTATTCCATTACAGAGCATCTCTGCTCCTGGCAGTGGGATCTGGAGCGACGGAAAAGATAA AAGCGAGGGGGCACAGGCACACACGTGGAATGTCGAGGTTTTGATTGATGTTCTTAAAGAGCTGAACCCG AGTTTGAATTTCAAGGAAGTAACTTATGAACTGGACCATCCTGGATTTCAGATTCGTGACAGCAAAGGAC TTCACAATGTGGTTTATGGCATTCAAAGGGGTCTGGGCATGGAAGTGTTTCCAGTAGATTTTATATATAG GCCTTGGAAACATGCAGAAGGCCAGCTCTCCTTCATCCAGCATTCCCTGATAAACCCAGAGGTCTTCTGT TTTGCTGACTACCCCTGTCACACTGTTGCCACTGATATTTTGAAAGCACCACCAGAGGATGACAATCGAG AAATTGCCACATGGAAAAGCTTGGATTTGATTGAATCTCTGCTGAGGCTTGCAGAGGTTGGGCAGTATGA GCAAGTGAAACAACTCTTCAGCTTCCCTATCAAACACTGTCCAGACATGTTGGTATTGGCCTTACTTCAA ATCAACACTTCCTGGCACACCCTACGCCATGAACTCATCTCTACTTTGATGCCAATTTTCCTTGGAAACC ATCCTAACTCTGCCATTATTTTGCACTATGCATGGCATGGGCAGGGGCAGTCTCCATCAATTCGCCAACT TATAATGCATGCAATGGCAGAATGGTACATGAGAGGGGAGCAGTATGATCAGGCCAAATTGTCTCGAATA CTTGACGTGGCCCAGGACTTGAAGGCCCTGTCGATGCTGCTGAACGGCACTCCATTTGCTTTTGTCATTG ACCTTGCTGCACTCGCTTCACGGCGGGAATACCTCAAACTTGACAAGTGGCTAACAGATAAGATAAGAGA GCATGGGGAGCCTTTTATACAAGCATGTATGACTTTTTTAAAGAGAAGGTGCCCTTCTATTTTGGGTGGA CTTGCCCCAGAGAAAGATCAGCCCAAAAGTGCTCAACTTCCCGCTGAAACTCTGGCAACAATGTTGGCTT GTCTGCAGGCTTGTGCAGGGAGTGTTTCTCAAGAGTTATCAGAGACCATCCTCACCATGGTAGCCAATTG CAGTAATGTAATGAATAAGGCCAGACAGCCACCACCTGGAGTTATGCCAAAAGGTCGCCCTCCTAGTGCT AGCAGCTTAGATGCCATTTCTCCTGTTCAGATTGACCCTCTTGCTGGAATGGCATCTCTTAGTATAGGTG GTTCGGCTGCCCCTCACACCCAGAGCATGCAGGGTTTTCCTCCAAATCTGGGTTCTGCGTTCAGTACCCC TCAGTCACCAGCAAAAGCATTTCCACCACTTTCAACCCCAAATCAAACAACGGCCTTCAGTGGGATTGGA GGACTCTCCTCACAGCTTCCAGTAGGTGGTCTTGGCACAGGAAGCCTGACTGGCATAGGAACTGGGGCCC TTGGACTTCCTGCAGTGAACAATGATCCTTTTGTACAGAGGAAACTGGGCACCTCTGGACTCAACCAGCC TACATTCCAGCAGACCGACTTGTCTCAGGTGTGGCCAGAGGCAAATCAGCACTTTAGTAAAGAAATTGAT GATGAAGCAAACAGTTATTTCCAGCGAATATATAATCACCCACCACATCCAACCATGTCTGTTGATGAGG TATTAGAAATGCTACAGAGATTTAAAGACTCTACAATAAAAAGGGAACGAGAAGTATTTAACTGTATGCT GAGGAACTTGTTTGAAGAATACCGGTTTTTCCCTCAGTACCCTGATAAAGAATTACATATAACAGCTTGC TTGTTTGGTGGCATAATTGAAAAAGGACTGGTCACTTACATGGCATTGGGTCTGGCCCTGCGATATGTTC TTGAAGCCTTACGGAAGCCTTTTGGATCCAAAATGTATTATTTTGGTATTGCTGCACTAGATAGATTTAA AAATAGATTGAAGGACTATCCTCAGTATTGTCAGCACCTGGCTTCTATCAGCCACTTTATGCAGTTCCCA CATCATTTGCAGGAGTACATTGAGTACGGACAGCAGTCTCGAGATCCTCCTGTGAAAATGCAGGGCTCTA TTACTACCCCTGGAAGTATTGCACTGGCTCAGGCCCAGGCTCAGGCCCAGGTTCCAGCAAAAGCTCCTCT TGCCGGTCAGGTTAACACAATGGTAACCACCTCAACAACCACCACTGTTGCTAAAACGGTTACAGTCACC AAGCCAACGGGAGTCAGCTTTAAGAAAGATGTGCCACCTTCTATAAATACAACAAATATCGATACATTGC TTGTGGCCACAGATCAAACTGAGAGGATTGTGGAGCCCCCAGAAAATATTCAAGAGAAAATTGCTTTTAT TTTCAACAATCTCTCCCAGTCAAATATGACACAGAAGGTTGAAGAGCTTAAAGAAACTGTGAAGGAAGAA TTTATGCCTTGGGTTTCCCAGTATCTGGTTATGAAAAGAGTCAGTATTGAGCCAAACTTTCATAGCCTGT ATTCAAACTTTCTTGATACATTGAAGAATCCTGAATTTAACAAGATGGTTCTGAATGAAACCTACAGAAA CATTAAAGTGCTGCTGACTTCTGATAAAGCTGCGGCCAATTTCTCAGATCGTTCTTTGCTGAAGAACTTG GGACATTGGTTAGGAATGATCACATTAGCTAAAAATAAACCCATCTTACACACAGACTTGGATGTGAAAT CTTTGCTGTTAGAGGCTTACGTTAAAGGACAGCAAGAGTTGCTCTATGTGGTGCCCTTTGTTGCCAAAGT TCTAGAGTCCAGCATTCGCAGCCTGGTTTTTAGACCACCAAACCCCTGGACAATGGCAATTATGAATGTA TTGGCTGAGCTGCATCAGGAGCATGACTTAAAGTTAAACCTGAAGTTTGAGATTGAGGTGCTCTGTAAGA ACCTTGCATTAGACATCAATGAGCTCAAACCTGGAAACCTGCTGAAAGATAAAGATCGTCTGAAGAACTT AGATGAGCAACTCTCTGCTCCAAAGAAAGATGTCAAGCAGCCCGAAGAGCTTCCTGCCATCACAACAACA ACAACTTCTACAACACCAGCTACCAGTACTACGTGTACAGCCACAGTTCCACCACAGCCACAGTACAGCT ACCACGACATCAATGTCTACTCTCTAGCAGGCCTGGCACCTCATATTACTCTAAACCCTACAATCCCTTT ATTTCAAGCTCATCCTCAGCTGAAGCAGTGTGTGCGGCAAGCAATTGAAAGAGCTGTCCAGGAGCTTGTC CATCCTGTGGTGGATCGCTCAATTAAGATTGCTATGACTACGTGCGAACAGATAGTCAGAAAGGACTTTG CCCTGGATTCTGAGGAGTCTCGAATGAGAATAGCAGCTCATCACATGATGCGGAACTTGACAGCTGGAAT GGCTATGATTACTTGCAGGGAACCTTTACTGATGAGCATATCCACCAACCTCAAAAACAGCTTTGCCTCA GCCCTGCGTACTGCATCCCCACAGCAAAGGGAAATGATGGACCAGGCTGCTGCTCAGTTAGCTCAGGACA ATTGTGAATTAGCTTGTTGTTTTATTCAGAAAACTGCAGTGGAGAAAGCAGGCCCTGAGATGGACAAGAG ATTGGCAACTGAATTTGAGCTGAGGAAGCATGCCCGGCAAGAAGGACGTAGGTACTGTGATCCTGTGGTC TTAACCTACCAAGCCGAGCGGATGCCGGAGCAGATCAGGCTGAAAGTTGGTGGTGTGGACCCAAAGCAGC TAGCCGTCTATGAAGAGTTTGCACGAAATGTGCCTGGCTTCTTACCTACAAATGACTTAAGTCAGCCTAC AGGGTTTTTAGCTCAGCCCATGAAGCAAGCTTGGGCAACTGATGATGTAGCGCAGATTTATGATAAGTGT ATTACAGAACTGGAGCAGCACCTACACGCCATTCCACCCACTCTGGCCATGAATCCTCAAGCCCAGGCTC TCCGAAGTCTTTTAGAGGTTGTAGTGTTGTCTCGGAATTCTCGGGATGCGATAGCTGCTCTTGGATTACT ACAGAAGGCTGTAGAAGGCTTATTAGATGCCACAAGTGGTGCTGACGCCGACTTACTGCTGCGATACAGG GAGTGCCACCTGTTGGTACTGAAAGCTCTGCAGGATGGCAGGGCATATGGATCTCCATGGTGCAACAAAC AAATCACAAGGTGCCTAATTGAATGTCGAGATGAATATAAATATAATGTGGAGGCTGTGGAATTGTTAAT TCGAAATCATTTGGTTAACATGCAGCAGTATGATCTTCATCTTGCTCAGTCGATGGAGAATGGCTTGAAC TATATGGCCGTGGCGTTTGCTATGCAACTGGTAAAAATTCTGCTGGTGGATGAAAGGAGTGTTGCCCATA TTACTGAGGCAGATCTGTTCCATACCATTGAAACCCTCATGAGAATTAATGCTCATTCCAGAGGCAATGC TCCAGAAGGGTTGCCCCAGCTGATGGAAGTGGTGAGATCCAACTATGAAGCAATGATTGATCGTGCTCAT GGAGGCCCTAACTTTATGATGCATTCTGGGATCTCTCAAGCCTCAGAATATGATGACCCTCCAGGCCTGA GGGAGAAGGCAGAGTATCTTCTGAGGGAATGGGTGAATCTCTACCATTCAGCAGCAGCTGGCCGCGACAG TACCAAAGCATTCTCTGCGTTTGTTGGACAGATGCACCAACAAGGAATACTTAAAACTGATGACCTCATA ACAAGGTTTTTTCGTCTTTGCACTGAAATGTGTGTTGAAATCAGTTACCGTGCTCAGGCGGAGCAGCAGC ACAATCCTGCGGCTAACCCTACCATGATCCGGGCCAAGTGCTACCACAACCTGGATGCCTTTGTCCGCCT CATTGCACTGTTAGTGAAGCACTCTGGGGAGGCCACCAACACTGTCACAAAGATCAACCTGCTCAATAAG GTTCTCGGTATAGTAGTGGGAGTTCTCCTACAGGATCATGATGTTCGGCAGAGTGAATTTCAGCAACTTC CGTACCATAGGATTTTTATCATGCTGCTGTTGGAGCTCAATGCACCCGAGCATGTGTTGGAAACCATTAA TTTCCAGACTCTTACTGCTTTCTGCAATACATTCCACATCTTGAGACCTACTAAAGCTCCTGGCTTTGTA TATGCTTGGCTCGAATTGATTTCCCATCGGATCTTTATTGCAAGAATGCTGGCACATACGCCACAGCAGA AGGGGTGGCCTATGTATGCTCAGCTGTTGATTGATTTATTCAAATATTTAGCACCTTTCCTTAGAAATGT GGAACTCACTAAACCTATGCAAATCCTCTACAAGGGTACTCTGAGAGTGCTGCTGGTTCTTTTGCATGAT TTCCCAGAGTTCCTTTGTGATTACCATTATGGGTTCTGTGATGTGATCCCTCCTAATTGTATCCAGTTAA GAAATTTGATCCTGAGTGCCTTCCCAAGAAACATGAGGCTCCCAGACCCATTCACTCCTAATCTGAAGGT GGACATGTTAAGCGAAATTAACATCGCACCTCGGATCCTCACCAATTTCACTGGAGTGATGCCGCCTCAG TTCAAAAAGGATTTGGATTCTTATCTTAAAACTCGGTCGCCGGTCACTTTCCTGTCTGATCTGCGTAGTA ACCTACAGGTATCTAATGAGCCCGGGAATCGCTACAACCTCCAGCTTATCAATGCATTGGTGCTCTACGT AGGGACTCAGGCTATCGCACACATCCACAACAAGGGCAGCACGCCCTCAATGAGCACCATCACCCACTCA GCACACATGGACATCTTCCAGAACCTGGCTGTGGACCTAGACACTGAGGGCCGATATCTCTTCTTAAATG CAATTGCGAACCAGCTACGGTACCCAAACAGCCACACTCACTACTTCAGCTGCACAATGTTGTACCTTTT TGCTGAGGCGAACACTGAGGCAATCCAGGAACAGATCACAAGGTTATTTCAGTCTGTTGCACAGTGCTGC ATGGGACAGAAGCAGGCTCAGCAAGTGATGGAGGGGACCGGTGCCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221036 representing NM_153164
Red=Cloning site Green=Tags(s) MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQ TQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSPD LRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTL RRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIMQF GVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSEGAQAHTWNVEVLIDVLKELNP SLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDFIYRPWKHAEGQLSFIQHSLINPEVFC FADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQ INTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRI LDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGG LAPEKDQPKSAQLPAETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSA SSLDAISPVQIDPLAGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIG GLSSQLPVGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQTDLSQVWPEANQHFSKEID DEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITAC LFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFP HHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVNTMVTTSTTTTVAKTVTVT KPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEE FMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNL GHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSLVFRPPNPWTMAIMNV LAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPAITTT TTSTTPATSTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELV HPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFAS ALRTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVV LTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKQAWATDDVAQIYDKC ITELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYR ECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLN YMAVAFAMQLVKILLVDERSVAHITEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAMIDRAH GGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLI TRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNK VLGIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFV YAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHD FPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQ FKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHS AHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRLFQSVAQCC MGQKQAQQVMEGTGAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_153164 |
ORF Size | 6978 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153164.4 |
RefSeq Size | 8391 bp |
RefSeq ORF | 6981 bp |
Locus ID | 234594 |
MW | 261.4 kDa |
Gene Summary | Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3' UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages.[UniProtKB/Swiss-Prot Function] |
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SDS |