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Npffr2 (NM_133192) Mouse Tagged ORF Clone
CAT＃: MR220938
- TrueORF®
Npffr2 (Myc-DDK-tagged) - Mouse neuropeptide FF receptor 2 (Npffr2)
ORF Plasmid: tGFP
"NM_133192" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gpr74; HG31; NPFF2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR220938 representing NM_133192
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAGAAATGGGACTCAAACTCTTCAGAAAGCTGGAATCACATCTGGAGTGGCAATGATACACAGC ATCACTGGTATTCAGATATCAACATTACCTATGTGAACTACTATCTCCACCAGCCCCAAGTGGCAGCTGT CTTCATCAGCTCCTACCTCCTGATCTTTGTCTTGTGCATGGTGGGAAATACTGTCGTTTGCTTTATTGTG ATAAGGAATAGACACATGCACACAGTCACTAATTTCTTCATCTTAAACCTTGCCATAAGTGATTTACTGG TTGGAATATTCTGTATGCCTATCACATTGCTGGACAACATCATAGCAGGATGGCCATTCGGAAGCAGCAT GTGCAAGATCAGTGGGCTGGTGCAAGGGATATCAGTTGCGGCTTCCGTCTTCACCTTGGTTGCAATAGCT GTGGACAGATTCCGCTGTGTGGTCTACCCCTTTAAGCCAAAGCTCACTGTCAAGACAGCCTTTGTCACGA TTGTGATCATCTGGGGCCTGGCCATCGCCATTATGACTCCATCTGCAATAATGTTACATGTACAAGAAGA AAAATACTACCGTGTGAGACTCAGCTCCCACAATAAAACCAGCACAGTCTACTGGTGTCGGGAGGACTGG CCAAGACACGAAATGAGGAGGATCTATACCACGGTGCTATTTGCCACCATCTATCTTGCTCCTCTCTCAC TCATTGTTATCATGTATGCAAGGATTGGGGCTTCCCTCTTCAAGACGGCAGCACACTGCACAGGCAAGCA GCGTCCAGTGCAGTGGCATGTATCCAAGAAGAAACAGAAGGTCATCAAGATGCTGCTGACTGTGGCCCTC CTTTTCATCCTTTCCTGGCTTCCCCTGTGGACCCTGATGATGCTCTCAGACTATACTGACCTGTCTCCTA ACAAACTGCGTATCATCAACATCTACATCTACCCTTTCGCCCACTGGCTCGCCTTCTGCAACAGCAGTGT CAACCCTATTATTTATGGATTCTTTAATGAAAATTTTCGCAATGGTTTCCAAGATGCTTTCCAGATCTGC CAAAAGAAAGCCAAGCCCCAGGAAGCCTATTCCCTGAGAGCGAAACGCAACATAGTCATAAACACATCGG GCCTGCTGGTGCAGGAACCGGTGTCTCAAAACCCAGGTGGGGAAAATTTGGGATGTGGAAAAAGTGCAGA CAATCCCACACAGGAATCCTTGATAGAGGAAATGGGAGAAGCTACTAACAGTACTGTGGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220938 representing NM_133192
Red=Cloning site Green=Tags(s) MSEKWDSNSSESWNHIWSGNDTQHHWYSDINITYVNYYLHQPQVAAVFISSYLLIFVLCMVGNTVVCFIV IRNRHMHTVTNFFILNLAISDLLVGIFCMPITLLDNIIAGWPFGSSMCKISGLVQGISVAASVFTLVAIA VDRFRCVVYPFKPKLTVKTAFVTIVIIWGLAIAIMTPSAIMLHVQEEKYYRVRLSSHNKTSTVYWCREDW PRHEMRRIYTTVLFATIYLAPLSLIVIMYARIGASLFKTAAHCTGKQRPVQWHVSKKKQKVIKMLLTVAL LFILSWLPLWTLMMLSDYTDLSPNKLRIINIYIYPFAHWLAFCNSSVNPIIYGFFNENFRNGFQDAFQIC QKKAKPQEAYSLRAKRNIVINTSGLLVQEPVSQNPGGENLGCGKSADNPTQESLIEEMGEATNSTVA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133192 |
| ORF Size | 1251 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133192.3, NP_573455.2 |
| RefSeq Size | 1643 bp |
| RefSeq ORF | 1254 bp |
| Locus ID | 104443 |
| UniProt ID | Q924H0 |
| MW | 47.9 kDa |
| Gene Summary | Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212100 | Npffr2 (untagged) - Mouse neuropeptide FF receptor 2 (Npffr2), (10ug) |
CNY 5,488.00 |
|
| MG220938 | Npffr2 (tGFP-tagged) - Mouse neuropeptide FF receptor 2 (Npffr2), (10ug) |
CNY 7,088.00 |
|
| MR220938L3 | Lenti ORF clone of Npffr2 (Myc-DDK-tagged) - Mouse neuropeptide FF receptor 2 (Npffr2) |
CNY 6,080.00 |
|
| MR220938L4 | Lenti ORF clone of Npffr2 (mGFP-tagged) - Mouse neuropeptide FF receptor 2 (Npffr2) |
CNY 7,888.00 |
