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Dgkz (NM_001166597) Mouse Tagged ORF Clone
CAT＃: MR220816
- TrueORF®
Dgkz (Myc-DDK-tagged) - Mouse diacylglycerol kinase zeta (Dgkz), transcript variant 1
ORF Plasmid: tGFP
"NM_001166597" in other vectors (4)
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CNY 7,824.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | E130307B02Rik; ENSMUSG00000075035; F730209L11Rik; mDGK[z] |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR220816 representing NM_001166597
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCTTCTTTAGGAAGCATTTCCAACGAAAGGCACGAGGTCCCGGAGAGAGGCAGCGGCGGTCCA GTGGCGTGGGGCTGCCCACAGGCAAGGCTCGACGCCGCTCCCCTGCAGGGCAGGCGTCCTCTTCACTGGC CCAGAGGCGCCGCTCCAGTGCACAGCTCCAGGGCTGTTTTCCCAGCTGCGGGGTGGGCACGAGAGGTGCC AGCCGCAGGCGATCCAGCACTGCACCCCCTACCTGCAATCCCCGCTTCATGGTGGATGTGGTGCCTACCC TACAGCCTGTCACAGTTGGGGCCCAGCTTCTGGGTGCACCCCTGCTGTTGGCTGGGCTCACAAGCATGAA GGAGGAAGAGGAGGAGGAAGGGGTCCAGGAGGGAAATGAGACAGCTGAGGCGACAAGTGATGCTAGGCCA GGTACCCGGACAACGCCCTTCTCACACCAGGGCACCTGGCCACTGCTTCCCATGCCCCGCTGCCTGCGTC GAGCCTCCTCCCACCTGCTCCCTGCTGATGTGGAGTGTGGCCATACCCTTTGGGGCCTGCATGGTCACTA TCGACGCCTCAGCCAGCGGTGGCCCTCAGGCCAGCACCCTGGTCTTGGGGGCCGAAGCCCAAGAGCCTCG GGCGCTGCAGCATGCCCCATGATACCGGCCCGTGTGCGCCCACTGTCTCGCAGACGCCAGGTAGCTCTAA GGCGGAAGTCAGCTGGACCCCAGCCCTGGAGCACCCTGCTTATGAAAGCCATCACCAAGTCGGGCCTCCA GCACCTGGCACCCCCTCCTCCCACGCCCGGGGCCCCGTGCGGTGAATCTGAGGAGCAGATCCAGAGCACT GTGGACTGGAGTGAGTCAGCAGTGTATGGGGAGCACATCTGGTTTGAGACCAACGTGTCCGGTGACTTCT GCTATGTCGGGGAGCAGCACTGTGTAGCTAAGATGCTGCCGAAGTCAGCGCCCAGAAAAAAGTGTGCAGC CTGTAAGATCGTGGTGCATACCCAATGCATTAAGCAGCTGGAAAAGATCAATTTCCGCTGTAAGCCGTCC TTCCGCGAATCAGGCTCCAGGAATGTCCGTGAGCCAACCTTCGTAAGACACCACTGGGTCCACAGACGAC GCCAGGATGGCAAGTGTCGGCACTGTGGGAAGGGCTTCCAGCAGAAGTTCACCTTCCACAGCAAGGAGAT CGTAGCCATCAGCTGCTCCTGGTGCAAACAGGCATACCACAGCAAGGTGTCGTGCTTCATGATGCAACAG ATTGAGGAACCCTGCTCCCTAGGGGTGCATGCAGCCGTGGTCATCCCACCCACCTGGATCCTGCGGGCCC GGAGGCCCCAGAACACCCTCAAGGCCAGCAAGAAGAAAAAGAGAGCGTCCTTCAAGAGGAGGTCCAGCAA GAAAGGACCTGAGGAAGGCCGCTGGAGACCCTTCATCATCAGACCCACCCCATCCCCCCTCATGAAACCC CTGCTGGTGTTTGTGAACCCCAAGAGTGGGGGCAACCAGGGTGCCAAGATCATCCAGTCTTTTTTGTGGT ATCTGAATCCCCGACAAGTCTTTGATCTGAGCCAGGGAGGACCCAGGGAAGCGCTGGAAATGTACCGCAA AGTACATAATTTGAGGATTCTGGCTTGCGGGGGTGATGGCACGGTTGGCTGGATTCTCTCCACCCTGGAC CAGCTGCGCTTAAAACCACCGCCTCCTGTAGCCATCCTGCCTCTGGGCACTGGCAATGACCTGGCCCGAA CCCTCAACTGGGGTGGGGGTTACACAGATGAGCCTGTGTCAAAGATCCTTTCCCATGTTGAGGAGGGGAA TGTGGTACAGCTGGACCGTTGGGACCTCCGAGCAGAGCCCAACCCTGAGGCGGGGCCTGAGGAGCGAGAT GATGGAGCCACTGACCGGCTGCCCCTGGATGTCTTTAACAACTACTTCAGCCTGGGCTTCGATGCCCACG TCACCCTGGAATTTCATGAGTCTCGAGAAGCCAACCCAGAGAAGTTCAACAGCCGCTTTCGGAATAAGAT GTTCTATGCTGGGACGGCCTTCTCTGACTTCCTGATGGGCAGCTCCAAGGACTTAGCCAAGCACATCCGA GTAGTGTGTGACGGAATGGACCTAACCCCCAAGATTCAGGACCTGAAACCGCAGTGCATCGTCTTTCTGA ATATCCCCAGGTACTGTGCAGGCACCATGCCCTGGGGCCACCCCGGGGAGCACCATGACTTCGAGCCCCA GAGGCATGATGATGGCTACCTGGAGGTCATCGGCTTCACCATGACATCCTTGGCAGCACTGCAGGTGGGT GGGCACGGCGAGCGATTGACGCAGTGCCGAGAAGTGCTGCTCACCACTGCCAAGGCCATCCCTGTGCAGG TGGACGGTGAGCCCTGCAAGCTTTCAGCATCACGGATTCGAATTGCCCTGCGCAACCAGGCCACTATGGT GCAGAAGGCCAAGCGCCGGAGTACTGCCCCACTGCACAGCGACCAGCAGCCAGTTCCTGAGCAGCTGAGG ATCCAGGTGAGCAGGGTCAGTATGCACGACTACGAGGCTCTACATTATGACAAGGAGCAGCTCAAGGAAG CCTCTGTGCCTCTGGGCACTGTGGTGGTCCCCGGAGACAGTGACCTGGAGCTCTGCCGCGCCCACATTGA GAGACTGCAGCGGGAGCCCGATGGGGCTGGAGCCAAGTCCCCGATGTGCCACCAACTCTCCTCCAAGTGG TGTTTCCTGGATGCCACCACTGCCAGCCGCTTCTACAGGATCGACAGAGCCCAGGAGCACCTCAACTATG TGACGGAGATTGCCCAGGACGAGATTTACATCCTAGACCCAGAGCTTCTGGGAGCATCAGCCCGGCCTGA CCTCCCCACCCCTACCTCCCCACTCCCTGCTTCCCCCTGCTCCCCTACACCCGGGTCAATGCAGGGGGAC ACTGCACTGCCCCAAGGTGAAGAACTGATTGAAGCAGCCAAGAGGAATGACTGCTGCAAGCTCCAGGAGC TACACCGAGCAGGAGGTGACCTCATGCACCGTGACCAGAAGAGCCGCACGCTCTTGCACCACGCGGTCAG CACTGGCAGTAAGGAAGTGGTCCGCTATCTGCTGGATCATGCACCTCCAGAGATCCTTGATGCTGTGGAG GAGAATGGGGAGACCTGTCTACACCAGGCAGCCGCCCTGGGTCAGCGCACCATCTGCCACTACATTGTGG AAGCCGGGGCCTCCCTCATGAAGACAGATCTGCAGGGCGACACTCCCCGGCAGCGCGCTGAGAAGGCTCA AGACACAGAGCTCGCTGCCTACCTGGAGAACAGACAGCATTACCAGATGATCCAGCGTGAGGACCAGGAG ACAGCTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220816 representing NM_001166597
Red=Cloning site Green=Tags(s) METFFRKHFQRKARGPGERQRRSSGVGLPTGKARRRSPAGQASSSLAQRRRSSAQLQGCFPSCGVGTRGA SRRRSSTAPPTCNPRFMVDVVPTLQPVTVGAQLLGAPLLLAGLTSMKEEEEEEGVQEGNETAEATSDARP GTRTTPFSHQGTWPLLPMPRCLRRASSHLLPADVECGHTLWGLHGHYRRLSQRWPSGQHPGLGGRSPRAS GAAACPMIPARVRPLSRRRQVALRRKSAGPQPWSTLLMKAITKSGLQHLAPPPPTPGAPCGESEEQIQST VDWSESAVYGEHIWFETNVSGDFCYVGEQHCVAKMLPKSAPRKKCAACKIVVHTQCIKQLEKINFRCKPS FRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMMQQ IEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRRSSKKGPEEGRWRPFIIRPTPSPLMKP LLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRKVHNLRILACGGDGTVGWILSTLD QLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLRAEPNPEAGPEERD DGATDRLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIR VVCDGMDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVG GHGERLTQCREVLLTTAKAIPVQVDGEPCKLSASRIRIALRNQATMVQKAKRRSTAPLHSDQQPVPEQLR IQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLQREPDGAGAKSPMCHQLSSKW CFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPASPCSPTPGSMQGD TALPQGEELIEAAKRNDCCKLQELHRAGGDLMHRDQKSRTLLHHAVSTGSKEVVRYLLDHAPPEILDAVE ENGETCLHQAAALGQRTICHYIVEAGASLMKTDLQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQE TAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001166597 |
| ORF Size | 3369 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001166597.1, NP_001160069.1 |
| RefSeq Size | 4090 bp |
| RefSeq ORF | 3372 bp |
| Locus ID | 104418 |
| MW | 125.1 kDa |
| Gene Summary | Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Regulates RASGRP1 activity (By similarity). Positively regulates insulin-induced translocation of SLC2A4 to the cell membrane in adipocytes. Activates PIP5K1A activity via generation of phosphatidic acid (PubMed:27739494).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223658 | Dgkz (untagged) - Mouse diacylglycerol kinase zeta (Dgkz), transcript variant 1, (10ug) |
CNY 14,350.00 |
|
| MG220816 | Dgkz (tGFP-tagged) - Mouse diacylglycerol kinase zeta (Dgkz) transcript variant 1, (10ug) |
CNY 9,220.00 |
|
| MR220816L3 | Lenti ORF clone of Dgkz (Myc-DDK-tagged) - Mouse diacylglycerol kinase zeta (Dgkz), transcript variant 1 |
CNY 10,260.00 |
|
| MR220816L4 | Lenti ORF clone of Dgkz (mGFP-tagged) - Mouse diacylglycerol kinase zeta (Dgkz), transcript variant 1 |
CNY 10,260.00 |
