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Ganab (NM_008060) Mouse Tagged ORF Clone
CAT＃: MR220524
- TrueORF®
Ganab (Myc-DDK-tagged) - Mouse alpha glucosidase 2 alpha neutral subunit (Ganab)
ORF Plasmid: tGFP
"NM_008060" in other vectors (4)
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CNY 7,072.00
CNY 8,460.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AU042638; G2an; GluII; mKIAA0088 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR220524 representing NM_008060
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAATAGCGGCAGTGGCGGCGCGTAGGAGGCGGTCTTGGTTAAGTTTGGTGCTGGCATACTTAG GGGTCTGTCTGGGGATTACACTTGCTGTGGATAGAAGCAACTTTAAGACCTGTGATGAGAGTTCCTTTTG CAAACGGCAGCGAAGCATTCGGCCAGGCCTCTCTCCTTACCGTGCCTTGCTGGACACTCTGCAGCTTGGT CCTGATGCTCTTACAGTCCATCTGATCCATGAAGTCACCAAGGTGCTGCTTGTGCTGGAGCTCCAGGGCC TTCAGAAGAACATGACTCGGATCAGGATCGATGAGCTAGAGCCCCGGCGGCCTCGATACCGAGTGCCAGA TGTTTTAGTGGCTGACCCCCCCACAGCTAGGCTTTCAGTCTCTGGCCGTGATGACAACAGTGTGGAGCTA ACAGTGGCTGAGGGACCCTACAAAATCATTTTGACAGCACAGCCATTCCGCCTTGACCTGCTAGAAGATC GAAGCCTCCTGCTCAGTGTCAATGCCCGAGGACTTATGGCCTTTGAGCACCAGAGGGCCCCCAGGGTCCC TTTCTCGGATAAAGTTAGTCTCGCGCTCGGTAGCGTGTGGGATAAGATCAAGAACCTTTTCTCTAGGCAA GAATCAAAAGACCCAGCTGAAGGCAATGGAGCCCAGCCTGAAGCAACACCTGGGGATGGTGACAAGCCAG AGGAGACCCAGGAAAAGGCTGAGAAGGATGAGCCAGGAGCCTGGGAGGAGACATTCAAAACACATTCTGA CAGCAAGCCTTATGGCCCCACGTCTGTAGGTTTGGACTTTTCTCTGCCAGGAATGGAACATGTGTATGGG ATCCCTGAGCATGCTGACAGCCTGAGACTGAAGGTCACTGAGGGCGGTGAGCCGTACCGCCTGTACAATT TGGATGTGTTCCAGTATGAGCTGAACAACCCCATGGCTCTATATGGGTCTGTGCCTGTGCTCCTGGCACA CAGCTTTCATCGAGACCTGGGCATCTTCTGGCTTAATGCTGCTGAGACTTGGGTTGATATATCCTCCAAC ACGGCTGGGAAGACCCTGTTTGGGAAGATGCTTGATTACCTGCAGGGCTCTGGGGAGACTCCACAGACAG ACATTCGTTGGATGTCAGAGAGTGGCATTATTGATGTTTTCCTAATGCTTGGCCCTTCGGTTTTTGATGT CTTTAGGCAGTATGCTAGTCTCACAGGGACCCAGGCATTGCCCCCACTCTTCTCCCTCGGCTATCACCAG AGTCGCTGGAACTACCGGGATGAGGCTGATGTTTTGGAAGTGGATCAGGGTTTTGATGATCACAACATGC CTTGTGATGTCATTTGGTTGGACATTGAACATGCTGATGGCAAGCGGTACTTCACTTGGGACCCCACCCG ATTTCCTCAGCCCCTCAATATGCTTGAGCACTTGGCTTCCAAGAGGCGGAAGCTGGTGGCCATTGTGGAC CCCCACATCAAGGTAGACTCTGGCTACCGAGTTCACGAAGAATTGCGAAACCATGGGCTGTATGTTAAAA CTCGGGATGGCTCTGATTACGAGGGCTGGTGCTGGCCAGGCTCAGCTAGTTACCCTGACTTCACTAATCC AAGGATGAGGGCCTGGTGGTCTAACATGTTCAGCTTTGACAATTATGAGGGTTCAGCTCCTAATCTTTAT GTTTGGAATGACATGAATGAACCGTCTGTGTTCAATGGTCCTGAGGTCACCATGTTGAAGGATGCTGTGC ATTATGGAGGCTGGGAGCACCGGGACATCCATAACATCTATGGCTTATATGTGCACATGGCGACTGCTGA TGGGCTAATACAGCGCTCTGGGGGCATAGAGCGTCCCTTTGTCCTGAGTAGGGCTTTCTTCTCAGGCTCC CAGCGCTTTGGAGCTGTGTGGACAGGGGACAACACTGCCGAATGGGATCATTTGAAGATCTCTATCCCTA TGTGTCTCAGCCTGGCACTGGTGGGGCTTTCCTTCTGTGGAGCGGATGTGGGTGGCTTCTTCAAGAACCC AGAGCCAGAGCTGCTTGTGCGCTGGTACCAAATGGGTGCCTACCAGCCGTTCTTTCGGGCTCATGCCCAC TTGGACACTGGGCGGCGAGAGCCATGGCTGTTAGCGTCTCAATACCAAGATGCAATCCGAGATGCCTTGT TCCAGCGATATTCTTTGCTGCCCTTCTGGTATACCCTCTTCTATCAAGCTCACAAGGAAGGGTTTCCTGT CATGAGGCCCCTCTGGGTACAGTATCCTGAGGATATGTCTACCTTCAGTATAGAGGATCAGTTCATGCTT GGTGATGCACTCCTTATTCACCCTGTATCGGATGCTGGGGCCCACGGAGTGCAGGTCTATTTGCCTGGCC AAGAAGAAGTGTGGTATGACATTCAGAGCTATCAGAAGCATCATGGGCCCCAGACCTTGTATCTGCCAGT AACTTTGAGCAGTATCCCCGTGTTCCAGCGTGGCGGAACCATTGTGCCTCGATGGATGCGTGTGAGGCGC TCTTCAGACTGTATGAAGGACGATCCTATCACTCTCTTTGTTGCTCTCAGTCCCCAGGGTACTGCCCAAG GAGAGCTCTTTCTAGATGATGGACACACCTTTAACTATCAGACTCGCCATGAGTTCCTGTTGCGGCGGTT CTCTTTCTCTGGCAGCACACTGGTCTCTAGTTCAGCAGACCCCAAAGGCCACCTTGAGACACCTATTTGG ATTGAGCGAGTAGTCATCATGGGGGCTGGAAAGCCAGCAGCTGTGGTGCTCCAGACCAAAGGATCCCCTG AAAGTCGCCTGTCCTTCCAGCATGACCCTGAGACCTCAGTGCTGATATTGCGTAAACCTGGTGTCAGCGT GGCATCCGACTGGAGTATTCATCTTCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220524 representing NM_008060
Red=Cloning site Green=Tags(s) MAAIAAVAARRRRSWLSLVLAYLGVCLGITLAVDRSNFKTCDESSFCKRQRSIRPGLSPYRALLDTLQLG PDALTVHLIHEVTKVLLVLELQGLQKNMTRIRIDELEPRRPRYRVPDVLVADPPTARLSVSGRDDNSVEL TVAEGPYKIILTAQPFRLDLLEDRSLLLSVNARGLMAFEHQRAPRVPFSDKVSLALGSVWDKIKNLFSRQ ESKDPAEGNGAQPEATPGDGDKPEETQEKAEKDEPGAWEETFKTHSDSKPYGPTSVGLDFSLPGMEHVYG IPEHADSLRLKVTEGGEPYRLYNLDVFQYELNNPMALYGSVPVLLAHSFHRDLGIFWLNAAETWVDISSN TAGKTLFGKMLDYLQGSGETPQTDIRWMSESGIIDVFLMLGPSVFDVFRQYASLTGTQALPPLFSLGYHQ SRWNYRDEADVLEVDQGFDDHNMPCDVIWLDIEHADGKRYFTWDPTRFPQPLNMLEHLASKRRKLVAIVD PHIKVDSGYRVHEELRNHGLYVKTRDGSDYEGWCWPGSASYPDFTNPRMRAWWSNMFSFDNYEGSAPNLY VWNDMNEPSVFNGPEVTMLKDAVHYGGWEHRDIHNIYGLYVHMATADGLIQRSGGIERPFVLSRAFFSGS QRFGAVWTGDNTAEWDHLKISIPMCLSLALVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAH LDTGRREPWLLASQYQDAIRDALFQRYSLLPFWYTLFYQAHKEGFPVMRPLWVQYPEDMSTFSIEDQFML GDALLIHPVSDAGAHGVQVYLPGQEEVWYDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRR SSDCMKDDPITLFVALSPQGTAQGELFLDDGHTFNYQTRHEFLLRRFSFSGSTLVSSSADPKGHLETPIW IERVVIMGAGKPAAVVLQTKGSPESRLSFQHDPETSVLILRKPGVSVASDWSIHLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008060 |
| ORF Size | 2898 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008060.2, NP_032086.1 |
| RefSeq Size | 3859 bp |
| RefSeq ORF | 2901 bp |
| Locus ID | 14376 |
| UniProt ID | Q8BHN3 |
| MW | 109.9 kDa |
| Gene Summary | Catalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins (PubMed:27462106). Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia (PubMed:28375157).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222863 | Ganab (untagged) - Mouse alpha glucosidase 2 alpha neutral subunit (Ganab), (10ug) |
CNY 12,350.00 |
|
| MG220524 | Ganab (tGFP-tagged) - Mouse alpha glucosidase 2 alpha neutral subunit (Ganab), (10ug) |
CNY 9,310.00 |
|
| MR220524L3 | Lenti ORF clone of Ganab (Myc-DDK-tagged) - Mouse alpha glucosidase 2 alpha neutral subunit (Ganab) |
CNY 10,360.00 |
|
| MR220524L4 | Lenti ORF clone of Ganab (mGFP-tagged) - Mouse alpha glucosidase 2 alpha neutral subunit (Ganab) |
CNY 10,360.00 |
