Top2a (NM_011623) Mouse Tagged ORF Clone
CAT#: MR219695
- TrueORF®
Top2a (Myc-DDK-tagged) - Mouse topoisomerase (DNA) II alpha (Top2a)
ORF Plasmid: tGFP
"NM_011623" in other vectors (4)
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CNY 15,200.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Top-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR219695 representing NM_011623
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTGTCACCGCTGCAGCCTGTAAATGAAAATATGCTAATGAACAAAAAGAAGAATGAAGATGGCA AGAAAAGATTGTCTATTGAAAGAATCTATCAGAAAAAAACACAACTGGAACATATACTGCTCCGCCCAGA TACCTACATTGGCTCTGTGGAGTTAGTGACCCAGCAAATGTGGGTTTACGATGAAGATGTTGGCATTAAC TACAGGGAAGTCACTTTTGTTCCTGGTTTGTATAAAATCTTTGATGAGATTCTAGTTAATGCTGCTGATA ACAAACAAAGGGACCCAAAAATGTCTTGTATTAGAGTCACAATTGATCCAGAAAATAATGTAATTAGCAT CTGGAATAATGGGAAAGGAATCCCTGTTGTTGAACACAAAGTTGAGAAAATATATGTCCCAGCTCTCATA TTTGGACAACTCCTGACCTCCAGTAACTATGACGACGATGAGAAGAAAGTGACAGGTGGTCGAAATGGCT ATGGAGCTAAACTGTGTAACATATTCAGCACCAAATTTACTGTGGAAACAGCCAGTAGAGAGTACAAGAA AATGTTCAAACAGACGTGGATGGATAACATGGGGAGAGCTGGTGACATGGAACTCAAGCCCTTTAGTGGA GAAGATTATACATGTATCACCTTCCAGCCTGACTTATCTAAGTTTAAAATGCAAAGCCTGGACAAAGATA TTGTTGCACTGATGGTCAGAAGAGCATATGATATCGCTGGCTCCACTAAAGATGTCAAAGTCTTTCTTAA TGGAAATAGTCTGCCAGTGAAAGGATTCCGCAGTTACGTGGATTTGTATTTGAAGGATAAGGTAGATGAA ACTGGGAATTCACTGAAGGTGATACATGAACAGGTCAATCCCCGGTGGGAAGTGTGCTTAACAATGAGCG AGAGAGGCTTCCAGCAGATTAGCTTCGTCAACAGCATTGCGACTTCTAAGGGCGGCAGACATGTCGATTA TGTAGCTGATCAGATTGTGAGCAAACTCGTTGATGTGGTGAAAAAGAAGAACAAGGGCGGGGTTGCAGTG AAGGCTCATCAGGTGAAAAATCACATGTGGATTTTTGTAAATGCCTTAATTGAAAACCCAACCTTTGACT CTCAAACAAAAGAAAACATGACTTTACAAGCCAAGAGCTTTGGATCAACATGTCAATTAAGTGAAAAGTT CATCAAAGCTGCAATTGGTTGTGGTATTGTGGAAAGCATACTAAACTGGGTGAAGTTCAAGGCTCAAATC CAACTGAACAAGAAGTGTTCAGCTGTAAAACATACCAAAATCAAGGGAATCCCCAAACTTGATGATGCCA ACGATGCAGGGAGCCGAAACTCTACTGAATGCACACTTATCCTGACTGAGGGAGACTCAGCAAAAACTCT AGCCGTTTCAGGCCTTGGAGTGGTTGGAAGAGACAAATATGGGGTGTTCCCTCTTAGAGGAAAGATACTC AATGTGCGAGAAGCCTCTCATAAACAGATCATGGAAAATGCTGAAATTAACAATATCATCAAGATTGTGG GTCTCCAGTACAAGAAAAACTATGAAGATGAAGATTCATTGAAAACTCTTCGTTATGGGAAGATCATGAT TATGACAGATCAGGACCAAGATGGTTCCCACATCAAAGGCTTGCTGATCAATTTTATCCATCACAATTGG CCATCTCTTCTGCGACATCGTTTTCTAGAAGAATTTATCACTCCCATTGTAAAGGTGTCCAAAAATAAGC AAGAAATAGCATTCTATAGTCTTCCCGAGTTTGAAGAATGGAAAAGTTCGACTCCGAATCATAAAAAGTG GAAAGTCAAATACTACAAAGGTTTGGGCACCAGCACATCAAAGGAAGCTAAGGAATATTTTGCAGATATG AAACGACATCGTATTCAGTTCAAATACTCTGGCCCTGAAGATGATGCTGCGATCAGCCTGGCCTTTAGTA AAAAACAAGTTGATGATCGAAAGGAATGGTTAACTAATTTTATGGAAGACAGAAGACAGCGGAAGTTACT TGGCCTTCCTGAGGATTATTTGTATGGACAAAGCACTTCGTACCTGACCTATAATGACTTCATAAACAAG GAACTCATCCTCTTCTCCAACTCTGATAATGAAAGGTCTATCCCATCCATGGTAGACGGTTTGAAACCAG GTCAGAGAAAGGTTTTGTTTACTTGTTTCAAACGGAATGACAAGCGAGAAGTGAAGGTTGCCCAGTTAGC TGGGTCAGTGGCAGAAATGTCCTCTTATCACCATGGTGAGATGTCACTGATGATGACCATTATCAATTTG GCGCAGAATTTTGTGGGTAGCAATAATTTGAACCTTTTACAGCCCATTGGTCAGTTTGGAACCAGGCTGC ATGGTGGCAAGGACTCAGCTAGTCCTAGGTACATCTTTACAATGCTCAGCCCTTTGGCTCGGTTGTTGTT TCCTCCAAAAGATGATCACACATTACGGTTTCTATATGATGACAACCAACGTGTTGAGCCTGAATGGTAC ATTCCTATAATACCCATGGTGCTGATAAATGGTGCTGAGGGAATTGGTACTGGGTGGTCCTGCAAAATCC CCAACTTTGATGTGCGTGAAGTTGTGAATAATATCAGACGGCTTCTGGATGGAGAGGAGCCCCTACCCAT GCTCCCAAGTTATAAGAATTTCAAAGGTACTATTGAAGAACTGGCTTCAAATCAGTATGTGATTAATGGA GAAGTAGCTATTCTGGATTCTACAACCATTGAAATCTCAGAGCTTCCCATCCGAACGTGGACTCAGACAT ATAAAGAACAGGTTCTAGAACCCATGTTGAATGGAACTGAGAAGACACCCTCTCTAATAACAGACTATAG GGAATACCATACAGATACCACTGTGAAGTTTGTCATAAAGATGACTGAAGAAAAACTGGCAGAGGCAGAG AGAGTCGGCCTACACAAAGTCTTCAAACTCCAGAGTAGTCTCACTTGCAACTCTATGGTGCTTTTTGACC ATGTAGGTTGCTTAAAGAAATATGACACTGTGTTGGATATTCTAAGAGACTTCTTTGAGCTCAGGCTTAA ATATTATGGATTAAGAAAAGAATGGCTTCTAGGAATGCTTGGTGCAGAATCTTCTAAACTGAATAATCAA GCTCGCTTTATATTAGAGAAAATAGATGGCAAAATAGTCATTGAAAATAAACCTAAAAAAGAATTAATTA AAGTTCTGATTCAGAGAGGCTACGACTCTGACCCTGTGAAAGCCTGGAAAGAAGCTCAGCAGAAGGTTCC AGATGAAGAAGAAAATGAAGAAAGTGACACTGAAACCAGCACCAGTGACTCTGCAGCCGAGGCTGGGCCG ACCTTCAACTACCTTCTCGACATGCCCCTGTGGTATCTGACCAAGGAGAAGAAGGATGAGCTGTGCAAAC AAAGAAACGAGAAGGAACAAGAGCTCAACACATTAAAGCAAAAGAGTCCATCAGATCTGTGGAAGGAAGA TCTGGCTGTTTTTATTGAAGAACTGGAGGTTGTCGAGGCCAAGGAAAAGCAGGATGAACAAGTGGGCCTT CCTGGAAAGGCGGGGAAAGCAAAGGGGAAGAAAGCACAGATGTGTGCTGACGTCTTGCCTTCTCCTCGGG GCAAAAGAGTCATCCCCCAAGTGACTGTGGAGATGAAAGCTGAGGCAGAGAAGAAAATTAGGAAGAAAAT TAAGAGTGAAAACGTTGAGGGTACCCCTGCTGAGGATGGTGCGGAACCAGGAAGCCTCCGGCAACGAATA GAGAAGAAGCAGAAGAAAGAGCCAGGTGCAAAGAAGCAGACTACATTGCCGTTTAAGCCTGTCAAAAAAG GGAGGAAGAAAAACCCCTGGTCTGACTCTGAGTCAGACGTGAGCAGTAATGAAAGTAACGTTGATGTCCC TCCTCGACAAAAAGAGCAAAGAAGTGCTGCAGCAAAAGCCAAATTCACAGTGGATTTAGACTCGGATGAA GATTTCTCAGGTTTGGATGAGAAGGATGAGGATGAAGATTTCCTCCCATTAGATGCTACTCCACCTAAGG CCAAAATCCCCCCAAAAAATACTAAAAAAGCACTGAAGACCCAGGGAAGCTCCATGTCGGTAGTTGATCT TGAAAGTGATGTTAAGGACAGTGTGCCAGCTTCTCCAGGCGTTCCTGCTGCTGACTTCCCAGCGGAAACT GAACAGTCAAAGCCATCCAAAAAGACCGTGGGAGTGAAGAAGACAGCAACCAAAAGCCAGTCTTCAGTCT CCACTGCTGGTACCAAAAAGAGAGCTGCGCCAAAGGGAACCAAATCAGATTCAGCCTTGAGTGCTCGTGT CTCGGAAAAACCTGCTCCTGCCAAAGCCAAGAACAGTCGCAAAAGGAAGCCATCTTCTTCTGATAGCTCT GACTCTGATTTTGAGAGAGCAATTTCTAAAGGTGCCACGAGCAAGAAAGCAAAGGGAGAGGAACAGGACT TCCCTGTGGACTTGGAAGACACGATAGCTCCTCGAGCCAAATCTGACCGGGCGAGGAAGCCAATTAAGTA CCTGGAGGAGTCCGATGACGATGACGACCTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219695 representing NM_011623
Red=Cloning site Green=Tags(s) MELSPLQPVNENMLMNKKKNEDGKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGIN YREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALI FGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSG EDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDE TGNSLKVIHEQVNPRWEVCLTMSERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAV KAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQI QLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKIL NVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNW PSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADM KRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINK ELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINL AQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWY IPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVING EVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAE RVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQ ARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAGP TFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVVEAKEKQDEQVGL PGKAGKAKGKKAQMCADVLPSPRGKRVIPQVTVEMKAEAEKKIRKKIKSENVEGTPAEDGAEPGSLRQRI EKKQKKEPGAKKQTTLPFKPVKKGRKKNPWSDSESDVSSNESNVDVPPRQKEQRSAAAKAKFTVDLDSDE DFSGLDEKDEDEDFLPLDATPPKAKIPPKNTKKALKTQGSSMSVVDLESDVKDSVPASPGVPAADFPAET EQSKPSKKTVGVKKTATKSQSSVSTAGTKKRAAPKGTKSDSALSARVSEKPAPAKAKNSRKRKPSSSDSS DSDFERAISKGATSKKAKGEEQDFPVDLEDTIAPRAKSDRARKPIKYLEESDDDDDLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011623 |
ORF Size | 4584 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011623.2, NP_035753.2 |
RefSeq Size | 5217 bp |
RefSeq ORF | 4587 bp |
Locus ID | 21973 |
UniProt ID | Q01320 |
MW | 173.2 kDa |
Gene Summary | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (PubMed:24321095).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224642 | Top2a (untagged) - Mouse topoisomerase (DNA) II alpha (Top2a), (10ug) |
CNY 19,570.00 |
|
MG219695 | Top2a (tGFP-tagged) - Mouse topoisomerase (DNA) II alpha (Top2a), (10ug) |
CNY 16,720.00 |
|
MR219695L3 | Lenti ORF clone of Top2a (Myc-DDK-tagged) - Mouse topoisomerase (DNA) II alpha (Top2a) |
CNY 17,100.00 |
|
MR219695L4 | Lenti ORF clone of Top2a (mGFP-tagged) - Mouse topoisomerase (DNA) II alpha (Top2a) |
CNY 17,100.00 |