Rhou (NM_133955) Mouse Tagged ORF Clone
CAT#: MR219421
- TrueORF®
Rhou (Myc-DDK-tagged) - Mouse ras homolog gene family, member U (Rhou)
ORF Plasmid: tGFP
"NM_133955" in other vectors (4)
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CNY 2,400.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2310026M05Rik; AI182090; Arhu; CDC42L1; G28K; mG28K; WRCH-1; WRCH1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR219421 representing NM_133955
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCGCAGCAAGGCCGGCCGGCGCTGCCCGCCCGCTGCGAGCCGCCGGCGGCGCCGCCGGTACCGC CTCGCCGAGAGCGCGGGGGGCGCGGGGCGCGCGGGCCCGGGGTGTCCGGGGGTCGGGGGCGCGCGGGCGG CGCCGAGGGACGCGGCGTCAAGTGCGTGCTGGTCGGCGACGGCGCGGTGGGCAAGACCAGCCTGGTGGTC AGCTACACCACTAACGGCTACCCCACCGAGTACATCCCTACGGCCTTCGACAACTTCTCGGCCGTGGTGT CTGTAGATGGGCGGCCTGTGAGACTCCAGCTCTGTGACACTGCAGGACAGGATGAGTTTGACAAGCTGAG GCCCCTCTGCTACACCAACACAGACATCTTCCTGCTGTGCTTCAGCGTGGTGAGCCCCACATCCTTCCAG AACGTGGGCGAGAAGTGGGTTCCAGAGATTCGACGTCACTGCCCAAAGGCCCCCATCATCCTGGTCGGGA CACAGTCGGACCTCAGGGAGGACGTCAAAGTGCTCATAGAACTGGACAAGTGCAAAGAGAAGCCGGTGCC TGAAGAGGCGGCGAAGCTGTGCGCGGAGGAAGTCAAAGCTGTCTCCTACATCGAGTGCTCAGCGTTGACT CAGAAAAACCTCAAAGAGGTTTTCGACGCCGCCATTGTTGCTGGTATCCAGCACTCAGACTCCCAGCTAC AGCCAAAGAAGTCTAAAAGCAGGACCCCGGATAAGGTGCGGGACCTGTCCAAGTCTTGGTGGAGGAAGTA TTGCTGCCTGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219421 representing NM_133955
Red=Cloning site Green=Tags(s) MAPQQGRPALPARCEPPAAPPVPPRRERGGRGARGPGVSGGRGRAGGAEGRGVKCVLVGDGAVGKTSLVV SYTTNGYPTEYIPTAFDNFSAVVSVDGRPVRLQLCDTAGQDEFDKLRPLCYTNTDIFLLCFSVVSPTSFQ NVGEKWVPEIRRHCPKAPIILVGTQSDLREDVKVLIELDKCKEKPVPEEAAKLCAEEVKAVSYIECSALT QKNLKEVFDAAIVAGIQHSDSQLQPKKSKSRTPDKVRDLSKSWWRKYCCLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_133955 |
ORF Size | 783 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133955.4, NP_598716.1 |
RefSeq Size | 3367 bp |
RefSeq ORF | 786 bp |
Locus ID | 69581 |
UniProt ID | Q9EQT3 |
MW | 28.8 kDa |
Gene Summary | Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
An RNAi screen unravels the complexities of Rho GTPase networks in skin morphogenesis
,null,
eLife
,PubMed ID 31556874
[Rhou]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210966 | Rhou (untagged) - Mouse ras homolog gene family, member U (Rhou), (10ug) |
CNY 3,990.00 |
|
MG219421 | Rhou (tGFP-tagged) - Mouse ras homolog gene family member U (Rhou), (10ug) |
CNY 2,850.00 |
|
MR219421L3 | Lenti ORF clone of Rhou (Myc-DDK-tagged) - Mouse ras homolog gene family, member U (Rhou) |
CNY 4,750.00 |
|
MR219421L4 | Lenti ORF clone of Rhou (mGFP-tagged) - Mouse ras homolog gene family, member U (Rhou) |
CNY 4,750.00 |