Awat2 (NM_177746) Mouse Tagged ORF Clone
CAT#: MR219385
- TrueORF®
Awat2 (Myc-DDK-tagged) - Mouse acyl-CoA wax alcohol acyltransferase 2 (Awat2)
ORF Plasmid: tGFP
"NM_177746" in other vectors (4)
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CNY 3,656.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 9430062J17Rik; ARAT; Dgat2l4; WS |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR219385 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTGGCCCACCAAGAAGGACCTCAAGACCGCCATGGAGGTCTTTGCTCTTTTCCAGTGGGCCCTCA GTGCCTTGGTTATAGTCACCACTGTGATCATTGTCAACCTCTACTTGGTGGTGTTCACATCTTACTGGCC TGTCACCGTGTTGATGCTCACCTGGCTGGCTTTTGACTGGAAGACCCCTGAGCGAGGTGGCCGCAGGTTC ACCTGTGTGAGGAAGTGGCGTCTGTGGAAACACTATAGCGACTACTTCCCACTCAAGATGGTGAAGACGA AAGATATTTCACCTGACCGCAACTACATCCTTGTCTGCCATCCTCATGGGCTCATGGCCCATTCATGTTT TGGTCACTTTGCCACTGACACAACAGGCTTTTCCAAGACCTTTCCTGGTATCACTCCTTATATGCTCACA CTAGGAGCCTTTTTCTGGGTACCTTTCCTCAGAGATTATGTAATGTCTACAGGGTCGTGCTCTGTGAGTA GATCCTCCATGGACTTTTTGCTTACCCAGAAGGGCACAGGCAACATGCTTGTGGTGGTGGTTGGTGGCCT GGCTGAGTGCAGATACAGCACGCCAGGCTCTACCACCCTGTTCTTGAAGAAGCGGCAAGGCTTTGTGCGC ACAGCCCTTAAACATGGGGTGTCTCTAATCCCAGCTTATGCCTTTGGAGAGACAGACCTCTATGACCAGC ACATTTTCACTCCTGGGGGCTTTGTCAATCGCTTCCAGAAGTGGTTCCAGAAGATGGTACACATCTACCC CTGTGCTTTCTATGGGCGTGGCCTCACCAAGAACTCCTGGGGCCTTCTGCCCTATTCTCAGCCAGTAACC ACTGTTGTTGGAGAACCTCTACCATTGCCCAAGATTGAGAATCCGAGCGAGGAGATTGTGGCCAAATACC ACACACTCTATATTGATGCCCTACGAAAATTGTTTGATCAGCATAAGACCAAGTTTGGCATCTCAGAGAC CCAGGAGCTGGTGATAGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219385 protein sequence
Red=Cloning site Green=Tags(s) MFWPTKKDLKTAMEVFALFQWALSALVIVTTVIIVNLYLVVFTSYWPVTVLMLTWLAFDWKTPERGGRRF TCVRKWRLWKHYSDYFPLKMVKTKDISPDRNYILVCHPHGLMAHSCFGHFATDTTGFSKTFPGITPYMLT LGAFFWVPFLRDYVMSTGSCSVSRSSMDFLLTQKGTGNMLVVVVGGLAECRYSTPGSTTLFLKKRQGFVR TALKHGVSLIPAYAFGETDLYDQHIFTPGGFVNRFQKWFQKMVHIYPCAFYGRGLTKNSWGLLPYSQPVT TVVGEPLPLPKIENPSEEIVAKYHTLYIDALRKLFDQHKTKFGISETQELVIV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_177746 |
ORF Size | 1002 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_177746.4, NP_808414.2 |
RefSeq Size | 1632 bp |
RefSeq ORF | 1002 bp |
Locus ID | 245532 |
UniProt ID | Q6E1M8 |
MW | 38.1 kDa |
Gene Summary | Acyltransferase that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that it plays a central role in lipid metabolism in skin. Has no activity using decyl alcohol and significantly prefers the C16 and C18 alcohols. May also have 2-acylglycerol O-acyltransferase (MGAT) and acyl-CoA:retinol acyltransferase (ARAT) activities, to catalyze the synthesis of diacylglycerols and retinyl esters; however this activity is unclear in vivo (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Allosteric modulation of the substrate specificity of acyl-CoA wax alcohol acyltransferase 2 (AWAT2)
,Arne, JM;Widjaja-Adhi, MA;Hughes, T;Huynh, KW;Silvaroli, JA;Chelstowska, S;Moiseenkova-Bell, VY;Golczak, M;,
J. Lipid Res
,PubMed ID 28096191
[Awat2]
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Documents
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FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC213221 | Awat2 (untagged) - Mouse acyl-CoA wax alcohol acyltransferase 2 (Awat2), (10ug) |
CNY 3,990.00 |
|
MG219385 | Awat2 (tGFP-tagged) - Mouse acyl-CoA wax alcohol acyltransferase 2 (Awat2), (10ug) |
CNY 3,710.00 |
|
MR219385L3 | Lenti ORF clone of Awat2 (Myc-DDK-tagged) - Mouse acyl-CoA wax alcohol acyltransferase 2 (Awat2) |
CNY 5,230.00 |
|
MR219385L4 | Lenti ORF clone of Awat2 (mGFP-tagged) - Mouse acyl-CoA wax alcohol acyltransferase 2 (Awat2) |
CNY 5,230.00 |