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Thbs4 (NM_011582) Mouse Tagged ORF Clone
CAT＃: MR219213
- TrueORF®
Thbs4 (Myc-DDK-tagged) - Mouse thrombospondin 4 (Thbs4)
ORF Plasmid: tGFP
"NM_011582" in other vectors (6)
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CNY 11,280.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TSP4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR219213 representing NM_011582
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGCCCCACGCGCGGCCGCGGCCGCCTTCCTCCTGCTGCACCTGGTCCTGCAGCCGTGGCAGCGGA CGAGCGCCCAGGCCACACCCCAGGTCTTTGATCTTCTACCGTCCTCCAGCCAGAGGCTGAACCCCAGCGC CCTGCAGCCAGTCCTGACAGATCCCACCCTGCACGAAGTCTATCTCATCTCCACCTTCAAGCTGCAGAGT AAAAGCTCAGCCACCATCTTTGGCCTCTACTCCTCAAGTGACAACAGCAAATACTTTGAATTCACTGTGA TGGGACGGCTGAACAAAGCCATCCTCCGCTACCTGAAGAATGATGGAAAGATCCATCTGGTGGTGTTCAA CAACTTGCAGCTGGCTGATGGGAGGCGCCACAGGGTTCTCCTGAGACTGAGCAATTTGCAGAGAGGAGAT GGCTCTGTAGAACTCTACCTGGACTGCGCTCAGGCGGACTCGGTGCGCAACCTCCCCAGAGCCTTTTCTG GCCTCACCCAGAACCCAGAGTCCATTGAATTGCGGACATTCCAGAGGAAGCCCCAGGACTTCTTGGAAGA GCTGAAGCTGGTGGTGCGGGGCTCGCTGTTCCAGGTGGCCAGCCTCCAAGACTGCTTCCTGCAGCAGAGT GAGCCTCTGGCTGCCACAAGCACAGGAGACTTTAATCGACAGTTCTTGGGTCAAATGACACAACTGAACC AGCTCCTTGGAGAGGTGAAAGATCTTCTGAGGCAGCAGGTCAAGGAAACATCGTTTTTGCGAAACACCAT TGCTGAATGCCAAGCTTGTGGTCCTCTCAGCTTTCAGTCCCCAACTCCAAACACACTGGTGCCCATAGCT CCTCCAGCACCCCCAACTCGCCCAACACGCCACTGTGACTCCAGCCCTTGTTTCCGAGGTGTCCGATGTA CGGACACCAGGGATGGCTTCCAGTGTGGACCCTGCCCAGATGGTTACACGGGAAACGGGATCACCTGCTC TGACGTGGATGAGTGCAAATACCATCCCTGCTATCCAGGTGTGCGCTGTGTGAATTTGGCTCCGGGTTTC AGATGTGACGCCTGTCCAGTAGGTTTCACAGGGCCCATGGTGCAGGGTGTCGGGATCAACTTTGCTAAAA CAAACAAGCAGGTGTGCACTGATGTTGATGAGTGTCAGAATGGAGCGTGTGTTCTGAATTCTATCTGCAT TAACACTTTGGGATCTTATCGCTGTGGGCCTTGCAAACCAGGGTACACTGGTGATCAGACGAGGGGATGC AAGACTGAAAGGAGCTGCCGGAACCCAGAGCAGAACCCATGCAGTGTGCATGCGCAGTGCATTGAGGAGC GGCAAGGGGATGTGACGTGTGTGTGTGGTGTTGGCTGGGCTGGTGACGGCTATGTCTGTGGAAAGGATGT GGACATTGACAGTTACCCTGATGAAGAACTGCCATGCTCTGCCAGGAACTGCAAGAAGGATAACTGCAAG TATGTGCCAAACTCTGGCCAGGAAGATGCAGACAGAGATGGCATCGGAGACGCGTGTGATGAGGATGCAG ATGGAGATGGGATCTTGAATGAACAGGACAACTGTGTCCTGACTCACAATATAGACCAAAGGAACAGTGA TAAAGATATCTTTGGGGATGCCTGTGACAACTGCCGGATGGTCCTGAATAATGACCAGAAGGATACTGAC GGGGATGGGAGAGGAGACGCCTGTGATGACGACATGGATGGAGATGGAATAAAAAACATTTTGGACAACT GTCCCAGAGTTCCCAACCGTGACCAGCAGGATCGGGATGGTGATGATGTCGGAGATGCTTGTGACAGCTG TCCTGATGTCAGTAACCCTAACCAGTCGGATGTGGATAACGATCTGGTTGGGGACTCCTGTGACACCAAC CAAGACAGTGACGGGGATGGCCACCAGGACAGCACAGACAACTGCCCCACAGTCATTAACAGCTCCCAGC TGGACACTGACAAAGATGGGATCGGCGATGAATGTGATGACGATGACGATAACGATGGCATCCCAGACCT GGTGCCTCCTGGCCCAGACAACTGCAGGCTCGTCCCCAACCCAGCCCAGGAAGATAGCAACAATGACGGG GTAGGAGATATCTGTGAAGCTGACTTCGACCAGGACCAGGTCATCGATCACATTGATGTGTGCCCTGAGA ATGCGGAGATCACCCTGACTGACTTCAGGGCCTACCAGACAGTGGTCCTGGACCCTGAAGGGGATGCTCA GATCGACCCCAACTGGGTGGTCCTGAACCAGGGCATGGAAATTGTGCAGACCATGAACAGTGACCCTGGC CTAGCTGTTGGGTACACGGCATTTAACGGAGTTGACTTTGAAGGGACCTTCCACGTAAACACCCAGACAG ATGATGACTATGCTGGCTTTATTTTTGGTTACCAAGACAGCTCCAGCTTCTATGTAGTCATGTGGAAGCA AACGGAGCAGACATACTGGCAGGCTACACCGTTCCGTGCAGTAGCAGAACCTGGTATTCAGCTCAAGGCT GTGAAGTCTAAAACTGGCCCAGGGGAACATCTCCGAAACTCCCTGTGGCACACAGGGGACACCAGCGACC AGGTCAGGCTGCTGTGGAAGGACTCCAGGAATGTGGGCTGGAAGGACAAGGTCTCCTACCGCTGGTTCCT GCAGCATAGGCCCCAGGTGGGCTACATCAGGGTGCGCTTTTATGAAGGCTCGGAGTTGGTGGCTGACTCT GGGGTCACCATAGACACCACCATGCGTGGAGGCCGACTTGGTGTGTTCTGCTTCTCTCAAGAAAACATCA TCTGGTCCAACCTCAAGTACCGCTGCAACGACACCATACCAGAGGACTTCCAGGAGTTCCAGACCCAGAG TTTCGACCGCTTGGATAAT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR219213 representing NM_011582
Red=Cloning site Green=Tags(s) MPAPRAAAAAFLLLHLVLQPWQRTSAQATPQVFDLLPSSSQRLNPSALQPVLTDPTLHEVYLISTFKLQS KSSATIFGLYSSSDNSKYFEFTVMGRLNKAILRYLKNDGKIHLVVFNNLQLADGRRHRVLLRLSNLQRGD GSVELYLDCAQADSVRNLPRAFSGLTQNPESIELRTFQRKPQDFLEELKLVVRGSLFQVASLQDCFLQQS EPLAATSTGDFNRQFLGQMTQLNQLLGEVKDLLRQQVKETSFLRNTIAECQACGPLSFQSPTPNTLVPIA PPAPPTRPTRHCDSSPCFRGVRCTDTRDGFQCGPCPDGYTGNGITCSDVDECKYHPCYPGVRCVNLAPGF RCDACPVGFTGPMVQGVGINFAKTNKQVCTDVDECQNGACVLNSICINTLGSYRCGPCKPGYTGDQTRGC KTERSCRNPEQNPCSVHAQCIEERQGDVTCVCGVGWAGDGYVCGKDVDIDSYPDEELPCSARNCKKDNCK YVPNSGQEDADRDGIGDACDEDADGDGILNEQDNCVLTHNIDQRNSDKDIFGDACDNCRMVLNNDQKDTD GDGRGDACDDDMDGDGIKNILDNCPRVPNRDQQDRDGDDVGDACDSCPDVSNPNQSDVDNDLVGDSCDTN QDSDGDGHQDSTDNCPTVINSSQLDTDKDGIGDECDDDDDNDGIPDLVPPGPDNCRLVPNPAQEDSNNDG VGDICEADFDQDQVIDHIDVCPENAEITLTDFRAYQTVVLDPEGDAQIDPNWVVLNQGMEIVQTMNSDPG LAVGYTAFNGVDFEGTFHVNTQTDDDYAGFIFGYQDSSSFYVVMWKQTEQTYWQATPFRAVAEPGIQLKA VKSKTGPGEHLRNSLWHTGDTSDQVRLLWKDSRNVGWKDKVSYRWFLQHRPQVGYIRVRFYEGSELVADS GVTIDTTMRGGRLGVFCFSQENIIWSNLKYRCNDTIPEDFQEFQTQSFDRLDN SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011582 |
| ORF Size | 2889 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011582.3, NP_035712.1 |
| RefSeq Size | 3202 bp |
| RefSeq ORF | 2892 bp |
| Locus ID | 21828 |
| UniProt ID | Q9Z1T2 |
| MW | 106.4 kDa |
| Gene Summary | Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222870 | Thbs4 (untagged) - Mouse thrombospondin 4 (Thbs4), (10ug) |
CNY 12,350.00 |
|
| MG219213 | Thbs4 (tGFP-tagged) - Mouse thrombospondin 4 (Thbs4), (10ug) |
CNY 9,310.00 |
|
| MR219213L1 | Lenti ORF clone of Thbs4 (Myc-DDK-tagged) - Mouse thrombospondin 4 (Thbs4) |
CNY 10,360.00 |
|
| MR219213L2 | Lenti ORF clone of Thbs4 (mGFP-tagged) - Mouse thrombospondin 4 (Thbs4) |
CNY 13,680.00 |
|
| MR219213L3 | Lenti ORF clone of Thbs4 (Myc-DDK-tagged) - Mouse thrombospondin 4 (Thbs4) |
CNY 10,360.00 |
|
| MR219213L4 | Lenti ORF clone of Thbs4 (mGFP-tagged) - Mouse thrombospondin 4 (Thbs4) |
CNY 10,360.00 |
