Hmmr (NM_013552) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AA386826; CD168; Rhamm
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR219106 representing NM_013552 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTTTCCTAAGGCGCCCCTGAAGAGATTCAATGACCCTTCGGGTTGTGCTCCATCTCCGGGTGCTT ATGATGTTAAAACTTCAGAAGCAACTAAAGGACCAGTGTCTTTTCAGAAATCACAAAGATTTAAAAACCA AAGAGAGTCTCAACAAAATCTTAACATTGACAAAGATACAACCTTGCTTGCTTCGGCTAAAAAAGCAAAG AAGTCTGTGTCAAAGAAGGACTCTCAGAAGAATGATAAAGATGTGAAGAGATTAGAAAAAGAGATTCGCG CTCTTTTGCAAGAGCGAGGGACTCAGGACAAACGGATCCAGGACATGGAATCTGAATTGGAGAAGACAGA AGCAAAGCTCAATGCAGCAGTCAGAGAGAAAACATCTCTCTCTGCGAGTAATGCTTCACTGGAAAAACGG CTTACTGAATTAACCAGAGCCAACGAGCTACTAAAGGCTAAGTTTTCTGAAGATGGTCACCAAAAGAATA TGAGAGCTCTAAGCCTGGAATTGATGAAACTCAGAAATAAGAGAGAGACAAAGATGAGGAGTATGATGGT CAAACAGGAAGGCATGGAGCTGAAGCTGCAGGCCACTCAGAAGGACCTCACGGAGTCTAAGGGAAAAATA GTCCAGCTGGAGGGAAAGCTTGTTTCAATAGAGAAAGAAAAGATCGATGAAAAATGTGAAACAGAAAAAC TCTTAGAATACATCCAAGAAATTAGCTGTGCATCTGATCAAGTGGAAAAATGCAAAGTAGATATTGCCCA GTTAGAAGAAGATTTGAAAGAGAAGGATCGTGAGATTTTAAGTCTTAAGCAGTCTCTTGAGGAAAACATT ACATTTTCTAAGCAAATAGAAGACCTGACTGTTAAATGCCAGCTACTTGAAACAGAAAGAGACAACCTTG TCAGCAAGGATAGAGAAAGGGCTGAAACTCTCAGTGCTGAGATGCAGATCCTGACAGAGAGGCTGGCTCT GGAAAGGCAAGAATATGAAAAGCTGCAACAAAAAGAATTGCAAAGCCAGTCACTTCTGCAGCAAGAGAAG GAACTGTCTGCTCGTCTGCAGCAGCAGCTCTGCTCTTTCCAAGAGGAAATGACTTCTGAGAAGAACGTCT TTAAAGAAGAGCTAAAGCTCGCCCTGGCTGAGTTGGATGCGGTCCAGCAGAAGGAGGAGCAGAGTGAAAG GCTGGTTAAACAGCTGGAAGAGGAAACGAAGTCAACTGCAGAACAACTGACGCGGCTGGACAACCTGCTG AGAGAGAAAGAAGTTGAACTGGAGAAACATATTGCTGCTCACGCCCAAGCCATCTTGATTGCACAAGAGA AGTATAATGACACAGCACAGAGTCTGAGGGACGTCACTGCTCAGTTGGAAAGTGTGCAAGAGAAGTATAA TGACACAGCACAGAGTCTGAGGGACGTCACTGCTCAGTTGGAAAGTGAGCAAGAGAAGTACAATGACACA GCACAGAGTCTGAGGGACGTCACTGCTCAGTTGGAAAGTGAGCAAGAGAAGTACAATGACACAGCACAGA GTCTGAGGGACGTCACTGCTCAGTTGGAAAGTGTGCAAGAGAAGTACAATGACACAGCACAGAGTCTGAG GGACGTCACTGCTCAGTTGGAAAGCTATAAGTCATCAACACTTAAAGAAATAGAAGATCTTAAACTGGAG AATTTGACTCTACAAGAAAAAGTAGCTATGGCTGAAAAAAGTGTAGAAGATGTTCAACAGCAGATATTGA CAGCTGAGAGCACAAATCAAGAATATGCAAGGATGGTTCAAGATTTGCAGAACAGATCAACCTTAAAAGA AGAAGAAATTAAAGAAATCACATCTTCATTTCTTGAGAAAATAACTGATTTGAAAAATCAACTCAGACAA CAAGATGAAGACTTTAGGAAGCAGCTGGAAGAGAAAGGAAAAAGAACAGCAGAGAAAGAAAATGTAATGA CAGAATTAACCATGGAAATTAATAAATGGCGTCTCCTATATGAAGAACTATATGAAAAAACTAAACCTTT TCAGCAACAACTGGATGCCTTTGAAGCCGAGAAACAGGCATTGTTGAATGAACATGGTGCAACTCAGGAG CAGCTAAATAAAATCAGAGACTCCTATGCACAGCTACTTGGTCACCAGAACCTAAAGCAAAAAATCAAAC ATGTTGTGAAATTGAAAGATGAAAATAGCCAACTCAAATCGGAGGTGTCAAAACTCCGATCTCAGCTTGT TAAAAGGAAACAAAATGAGCTCAGACTTCAGGGAGAATTAGATAAAGCTCTGGGCATCAGACACTTTGAC CCTTCCAAGGCTTTTTGTCATGCATCTAAGGAGAATTTTACTCCATTAAAAGAAGGCAACCCAAACTGCT GC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR219106 representing NM_013552 Red=Cloning site Green=Tags(s) MSFPKAPLKRFNDPSGCAPSPGAYDVKTSEATKGPVSFQKSQRFKNQRESQQNLNIDKDTTLLASAKKAK KSVSKKDSQKNDKDVKRLEKEIRALLQERGTQDKRIQDMESELEKTEAKLNAAVREKTSLSASNASLEKR LTELTRANELLKAKFSEDGHQKNMRALSLELMKLRNKRETKMRSMMVKQEGMELKLQATQKDLTESKGKI VQLEGKLVSIEKEKIDEKCETEKLLEYIQEISCASDQVEKCKVDIAQLEEDLKEKDREILSLKQSLEENI TFSKQIEDLTVKCQLLETERDNLVSKDRERAETLSAEMQILTERLALERQEYEKLQQKELQSQSLLQQEK ELSARLQQQLCSFQEEMTSEKNVFKEELKLALAELDAVQQKEEQSERLVKQLEEETKSTAEQLTRLDNLL REKEVELEKHIAAHAQAILIAQEKYNDTAQSLRDVTAQLESVQEKYNDTAQSLRDVTAQLESEQEKYNDT AQSLRDVTAQLESEQEKYNDTAQSLRDVTAQLESVQEKYNDTAQSLRDVTAQLESYKSSTLKEIEDLKLE NLTLQEKVAMAEKSVEDVQQQILTAESTNQEYARMVQDLQNRSTLKEEEIKEITSSFLEKITDLKNQLRQ QDEDFRKQLEEKGKRTAEKENVMTELTMEINKWRLLYEELYEKTKPFQQQLDAFEAEKQALLNEHGATQE QLNKIRDSYAQLLGHQNLKQKIKHVVKLKDENSQLKSEVSKLRSQLVKRKQNELRLQGELDKALGIRHFD PSKAFCHASKENFTPLKEGNPNCC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_013552
ORF Size	2382 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013552.2, NP_038580.2
RefSeq Size	3914 bp
RefSeq ORF	2385 bp
Locus ID	15366
UniProt ID	Q00547
MW	92.2 kDa
Gene Summary	Receptor for hyaluronic acid (HA) (PubMed:1376732). Involved in cell motility (PubMed:1376732). When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. May act as a regulator of adipogenesis (PubMed:22666460).[UniProtKB/Swiss-Prot Function]

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