Mrps27 (NM_173757) Mouse Tagged ORF Clone
CAT#: MR219080
- TrueORF®
Mrps27 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein S27 (Mrps27), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_173757" in other vectors (4)
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CNY 4,180.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2610028H14Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR219080 representing NM_173757
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTCCCATGGTGCGGTGTGGGATGCTCCTGGCTCGGCGACTAGACGCGTCTCGCCTTTGCCTCG CAGGTAAAAGATGCCTGCTTTCTGCAGCGTACGTGGACAGCCACCAGTGGGAAGCCAGAGAGAAGGAGGA ATACCATCTGGCTGATCTTGCATCCTTAATGGACAAAGCGTATGAAAGAAAATTGCCTGTTAGTTCTTTA TCAATATCACGGTTTGTGGACAACATTGCATCCCGAGAAGATCTAGATTCTGCAGAGTACTATCTTTACA AATTTCGACACAGTCCCAACTGCTGGTACCTAAGAGACTGGACCATCCACAGCTGGATTAGACAGTGTCT GAAATACGGAGCACAAGACAAAGCCTTGTATACACTTGTTAATAAGGTTCAGTATGGAATTTTTCCAGAT AACTTTACATTCAACTTACTGATGGATTATTTCATAAAGAAAGGAAATTACAAAGATGCTTTATCCGTGG TTTTTGAGATCATGATGCAAGAAGCCTTTGACGTGCCGTCCACCCAGTTTCTCTCCCTCTATGTTTTATA CCGTTGCCTAGCAGAGAAGACGGAGCTCACATGGGAAGAAGAAAGAGACTTTGGGGCGTCCCTCTTGCTT TCTGGCCTGAAACAAAGGAACACAGTGGGCCTGAGTTCCCAGCTGTATGGCTATGCACTTCTCGGGAAGG TGGAACTGCAGCGTGGGGTGCGGGCTGTATACCACGGCATGCCTCTGATGTGGACACCAGGCTACCTCGA CAGAGCCCTCCAAGTGATGGAGAGGGTGGCCTCCTCCCCTGAAGACCTCAAGCTGGGTAGAGAAGTGCTC GATGTGCTGGATGGAGTCCTGAAGGTGGTGACGTCTCCAGATGTCCAGACTTCAGAGGCACAGCCTCAGG AAGGTGAAGATGGCTTGGGCTCAGCAAACCTGGTGGAGCAGCTAGACACTGAGGAACCAGAACAGTCCAA GCTTCCCCGATACCTCGAGCGCTTTCAGGCCTCTCGGTCTAAACTGCAGGAGCTGAACAGAGTTGAGTCT GAAAGTCTTTTGACTCTGACCACCCAGCTTGTAAAAGAAAAACTCCCCGCCTGTGAGGCCGAGGACCTGG CCACCTACGAGCAGAAACTCCGTGAGTGGCACCTGGAGCGGGTGCAGTTGATCCAGAGGGAGCAGGAACA GAGGGAGAAGGCCAAGCAAGAGTACCAGGCGCTGAGTGCGGCAGAGAAAGCAGCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR219080 representing NM_173757
Red=Cloning site Green=Tags(s) MAAPMVRCGMLLARRLDASRLCLAGKRCLLSAAYVDSHQWEAREKEEYHLADLASLMDKAYERKLPVSSL SISRFVDNIASREDLDSAEYYLYKFRHSPNCWYLRDWTIHSWIRQCLKYGAQDKALYTLVNKVQYGIFPD NFTFNLLMDYFIKKGNYKDALSVVFEIMMQEAFDVPSTQFLSLYVLYRCLAEKTELTWEEERDFGASLLL SGLKQRNTVGLSSQLYGYALLGKVELQRGVRAVYHGMPLMWTPGYLDRALQVMERVASSPEDLKLGREVL DVLDGVLKVVTSPDVQTSEAQPQEGEDGLGSANLVEQLDTEEPEQSKLPRYLERFQASRSKLQELNRVES ESLLTLTTQLVKEKLPACEAEDLATYEQKLREWHLERVQLIQREQEQREKAKQEYQALSAAEKAA SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_173757 |
ORF Size | 1245 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_173757.3, NP_776118.2 |
RefSeq Size | 1849 bp |
RefSeq ORF | 1248 bp |
Locus ID | 218506 |
UniProt ID | Q8BK72 |
MW | 48.2 kDa |
Gene Summary | RNA-binding component of the mitochondrial small ribosomal subunit (mt-SSU) that plays a role in mitochondrial protein synthesis. Stimulates mitochondrial mRNA translation of subunit components of the mitochondrial electron transport chain. Binds to the mitochondrial 12S rRNA (12S mt-rRNA) and tRNA(Glu). Overexpressed in hepatocellular carcinoma tissues compared with adjacent non-tumoral liver tissues.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212688 | Mrps27 (untagged) - Mouse mitochondrial ribosomal protein S27 (Mrps27), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 3,990.00 |
|
MG219080 | Mrps27 (tGFP-tagged) - Mouse mitochondrial ribosomal protein S27 (Mrps27) nuclear gene encoding mitochondrial protein, (10ug) |
CNY 4,560.00 |
|
MR219080L3 | Lenti ORF clone of Mrps27 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein S27 (Mrps27), nuclear gene encoding mitochondrial protein |
CNY 6,080.00 |
|
MR219080L4 | Lenti ORF clone of Mrps27 (mGFP-tagged) - Mouse mitochondrial ribosomal protein S27 (Mrps27), nuclear gene encoding mitochondrial protein |
CNY 6,080.00 |