Aplf (NM_001170489) Mouse Tagged ORF Clone
CAT#: MR218907
- TrueORF®
Aplf (Myc-DDK-tagged) - Mouse aprataxin and PNKP like factor (Aplf), transcript variant 1
ORF Plasmid: tGFP
"NM_001170489" in other vectors (3)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2010301N04Rik; AI452191 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR218907 representing NM_001170489
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTCGGACTTCTTTCTGCAGCCTCTGGACGGCGGTCCCCGCGTGCCCGTGGGGCCGGGGCAGACTG TGATAGGCCGCGGGCCGCTGCTGGGAATAACAGACAAAAGAGTATCCAGAAGACATGCCATTCTTGAAGT GGTTGATAGTCAGCTTCGAATCAAGCCAATCCATAGGAATCCATGTTTCTACCAGTCCTCGGAGAAGAGC CAACACTCGCCAATGGAGACGCAAGTGTGGAGCCAGTTGCATCCCGGGGATAGCTTTTCCCTGTTACTTG ACAAGTACGCTTTCCGTGTTTTCTCTGCAGAATCTGAAGTGGAGATGGAGTGTACTCTGAGAAACAGTCA GATGCTTGATGAAGATGATATATTGAGTGAGATGCAGAAGTCCCCTGTGGTTAACTTACCTGATAAGACA ACTGGTGCCTCACAGCTGCAAGGAAGCCCTGAAATAACCAAGACCAAGTGTCCCACCATAGATCCTATGT CCTCTTCAGGTGAATGCAGAGCTTTCAGTGAGCACCAGCCACGTCCTACCCAGAGGAAAAGAATCCTCCC AGCTTGGATGTTGGCGGAAAGTCTAAGTGACCAGAGCCTCTCCACACCAGCTGAGGGGGGAGATAAGGAT GTCATCCAGAGGAGTGGAAAAGCAGGAACCTGTGAAGATAGAACCCCAGGAAACACATCATGGCATGGGA AAAAGCGGCTTAGTCCATCAGGAAATTCAAAAAGTGTGTCAGCGGAACAAGACCCTGGGAAGAAGTGCAG AAAGGCTGACCAAGAAGGACCGGGTGTTTCTTCTGAGAATGTTCCAGAATCATCATCTTCAAACATAGTA AAGGACCCAGATGTGGATATTGTGAAGACAAATAAACAGAAGGACGGGATTTTAATAGAGGAACTTGGTG AGGTCTCTAAACATAAAGCCGCCACCAAACCAACCACAAATGAAGAGGGCGAGAGTTGTGCACGTGTCCA AAGCAAGTCACCTCCTGAGAAATCTCAAGGATGCCATCCTGAGTCTAGCTCTGCTCCCTCCAGTCCTGAT GCTTTGCACACAGACACAGCTGACCCAGTGCTGGGCTGTTCAGAAGAAAGCAAGGTCCGGAGGACGGCCT GCATGTATGGGGCCAACTGCTACAGGAGGAACCCCCTCCACTTTCAACACTTCAGCCACCCTGGTGACAG TGACTATGGAGAGGTACATGGCACAGATGAAGGTGTGATTGGTGACAGGCCCGAGTGTCCCTATGGAGCG TCTTGTTACAGGAAGAACCCCCAGCACAAGATGGAATATAGGCACAGTGCACTTCCAGCCAGAGTTGCTC TGGATGAAGATGACGATGATGTCGGGCAACCCAGTGACGATGAGGACGAGGAAGACTATGAGCCGACAGA TGAAGATTCTGATTGGCACCCAGGAAAGGACGATGAGGAGCAGGAAGATGTGGATGAGCTTTTGAAAGAA GCAAAAAGTTCTTTGCACTTGAAACAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218907 representing NM_001170489
Red=Cloning site Green=Tags(s) MPSDFFLQPLDGGPRVPVGPGQTVIGRGPLLGITDKRVSRRHAILEVVDSQLRIKPIHRNPCFYQSSEKS QHSPMETQVWSQLHPGDSFSLLLDKYAFRVFSAESEVEMECTLRNSQMLDEDDILSEMQKSPVVNLPDKT TGASQLQGSPEITKTKCPTIDPMSSSGECRAFSEHQPRPTQRKRILPAWMLAESLSDQSLSTPAEGGDKD VIQRSGKAGTCEDRTPGNTSWHGKKRLSPSGNSKSVSAEQDPGKKCRKADQEGPGVSSENVPESSSSNIV KDPDVDIVKTNKQKDGILIEELGEVSKHKAATKPTTNEEGESCARVQSKSPPEKSQGCHPESSSAPSSPD ALHTDTADPVLGCSEESKVRRTACMYGANCYRRNPLHFQHFSHPGDSDYGEVHGTDEGVIGDRPECPYGA SCYRKNPQHKMEYRHSALPARVALDEDDDDVGQPSDDEDEEDYEPTDEDSDWHPGKDDEEQEDVDELLKE AKSSLHLKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001170489 |
ORF Size | 1497 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001170489.1, NP_001163960.1 |
RefSeq Size | 3184 bp |
RefSeq ORF | 1500 bp |
Locus ID | 72103 |
UniProt ID | Q9D842 |
MW | 55.4 kDa |
Gene Summary | Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG218907 | Aplf (tGFP-tagged) - Mouse aprataxin and PNKP like factor (Aplf) transcript variant 1, (10ug) |
CNY 3,710.00 |
|
MR218907L3 | Lenti ORF clone of Aplf (Myc-DDK-tagged) - Mouse aprataxin and PNKP like factor (Aplf), transcript variant 1 |
CNY 5,230.00 |
|
MR218907L4 | Lenti ORF clone of Aplf (mGFP-tagged) - Mouse aprataxin and PNKP like factor (Aplf), transcript variant 1 |
CNY 5,230.00 |