United States
Germany
Japan
United Kingdom
China
Pmfbp1 (NM_019938) Mouse Tagged ORF Clone
CAT＃: MR218887
- TrueORF®
Pmfbp1 (Myc-DDK-tagged) - Mouse polyamine modulated factor 1 binding protein 1 (Pmfbp1)
ORF Plasmid: tGFP
"NM_019938" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,128.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1700016D22Rik; F77; Stap |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218887 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAAAGCTTAAAGGAGAATTACGAACTGCCAAGGGACTGAAGAATGAGGCTGGGGAGAGAGACAGGG ACGTCAGCAATCTAAACAGCAAGCTCTTGAGCCTACAACTGGACATCAAGAATCTCCACGATGTCTGCAA GAGGCAGGGGAAGACCTTGCAAGAGAACCAGCTCTGCGTGGAGGAAGCGATGCTGAAAGCCAACCACAAC AAGAAGCAAGCCCAGACGTTAGTATTCACGGACAACCAGATGGACTTCCGGGTCAATAAGCAATACCATC TCAGGCAACTCCAGCAACTGAAGAAGAAGCTGCTGACCCTCCAGCAGGAACTGGAGTTCCGTACCCAGGA GCTCCAGGCTTCCTACTGCTCTCTCCTGCAGTACCAGTCGATCCTGGAGAAGCAGACTTCCGACCTTCTT GTTCTTCACCGTCACTGTAAACTTAAGGAAGATGAGGTCATTCTCTACGAGGAGGAGATGGGAAATCATA GTAAGAGCACGGGGGAGAAACTCCATCTGGCGCAAGAGCAGCTTGCCTTGGCGGGGGACAAGATCCTCTC CCTAGAAAGGAGCCTAAACCTCTACAGGGACAAGTACCAGACTTCCCTGAGCAACATAGAGTTACTGGAG TGCCAAGTGAAGATGCTGGAGGGGGAACTCAGCGGACTCATTGGTCAGGACCCTGAGAACAAAGGAGACC ACCCGAAGGTTCGAATCTACACTTCTCCGTGCGTGATTCAAGAGCATCAGGAGACCCTAAAGCGACTGTC TGAAGTCTGGCAAAAGGTTTCTGAACAGGATGACCTAATCCAGGAGCTTCGGAATAAACTGGCCTGCAGC AACTCTTTGGTCCTGGAGCGTGAGGAGGCGTTGATAAAATTACAAGCTGAGTTTGCTTCCTACACAGCCA CCCACCGGCATCCCCCTACCTCCTCAGAAGACTGTGAAGACATCACAAAGATCCTGAAGCACCTGCAAGA GCAGAAAGACAGCCAGTGCCTGCACGTGGAGGAGTACCAGAACCTTGTGAAGGACCTGCGCATGGAGCTG GAAGCTGTGTCAGAACAGAAGAAAAAGATCATGAAGGACATGATGAAGCTGGAGCTAGATCTACATGGGC TGCGGGAAGAGACGTCCTGCGTCATCGAGAAGAAGGACAAGGAGACTGTCTTCCTGCAGTACCGGCTGCA AGATCTGCAGCAACAGTACACAGAGTCACAGAAGCTGTCCCTGAAGAAGGACAAGTTGCTCCAAGACAAG GATGAGAGGCTGCACGAGCTGGAGAAGAATCTGATGCAGGTGCAGAACAGCCTCAGAGAGAAAGAGGCAG AGCTGGAGAAGCTACAGTGTACAACCAAAGAACTGGACACCAGCCTCCAAGAAGCTAGGCAAAGCACATC CAAAATAGACTGCGAGGCCCTGCGGGCCGAGATCCAGAAGCTCAAGGATAGCCTCGAGGAGGCCAGGGAG CAGCTGAAGGTGTCAGATCAGAATCTAACCCAGAGTAAGGAGGAAGCGCACCTCTCTGCCAGCAGCCTAG AGGATGCTCATCGGAAAATCGAGAACTGCCTTCTCCAGGACAAGCAGAAGGAAGAGGTCATTAAGGATCT GCAGAGCCAGTTGCATAAGCTACAGAAGGAGTCTTCGAAAATTGAGGAGGAACGAAAACACAACAGGCAA CGGTTACAGGAGCTGAGCTCGGAACTCTCTGAAGGCCAGAGAAGGCTTTCGAATGCAGAGAAGGAAAAGT CGCTGCTTCAGAAGACACTGGATGAAGACGAGAAAAAGATAGATGAGCTATTTCACAGCACCCAAGTCTC TGAGCAAAAGCAAAGGGAGCTAACAAACTCCATAAGGAAGCTAGAGGAGGAACTTCTGGAGATAAAAGGG CTCTTGGAGGAGAAACGAGAGCAACTGAAGAAGAGTAAGGAACAGGAGAAGGCACTGGAGGAAGAGATCG AGGCTTTGCGACAAGAAGCGAAAAGGAAAGAGAAGATGGCGAAGGAACATTTGAGAAAGCTGGATGAAGA GAAGGAGAACCTGCAGGCTGAGTTGACCTCCCGTTCCTCACACCTGGACTCCTCTCTCAACAAATACAAC TCCTCCCAGAAAGTCATCCAAGAGCTCAACGCGGAGATAGCCCGCCAGAAGGATTCCATCATGATTCTGC AGACACAGCTGGATTCGGCTATCCAGAAAGAGAAGAACTGTTTCCAGAACATGGTTAGTAAAGAAGCTTA TGAGGAACTTGTGCGGAAGTCGGGCAACTGCCAGGACGACCTGACACAGGCCCTGGAGAAGCTCACTCAG GCAACCTCAGAGACAAAGAGCCTGAATCGCTCCTTGCAACAGACCCAGGAGAGGAAAGCTCAACTGGAAG ATGAAATCGCGGCTTATGAGGAAAGGATGAAAAAGCTCAATATGGAGTTAAAAAAACTGCAGGGCTTCCA ACAGCAGAGCGAGCAAGAGGTGCACAACTTTGACAAGAAGCTGGAGGAGATGAGCAACCAGGTGTTGCAG TGGCAGAGGCAGCACCAGAGTGACCTTAAGATGTTGGCAGCTAAGGAGTCGCAGCTCCGAGAATTCCAGG AGGAAATGGCGACCCTAAGAGAGAGCCTCCTTGCAGATGAGAAGGAGCCGTACTCCCCGCCTGCAAAGCT AACCCCCAAAGAAACCTGCAGGCACCACCGGGAGAATGACCAGATTATGAACAACGTGGAACAATGGGCA AAGGAGCAGAAGATTGCCAATGAGAAGTTGGGAAACAAGCTGAGAGAGCAGGTTAAATACATTGCCAAGT TGACTGGCGAAAAGGACCACCTCCACAATGTAATGGTCCACCTGCAGCAGGAGAACAAAAAGCTGAAGAA CGAGATAGAAGAGAAGAAGCTAAAGGCCGGGAACCCGAGGATCTGTGTCAAGGCCTTTCCCCCAAACAAA CTGGAGCCTTCCCAAAAGGGAAAGCTATGCTGTGCCCTGGGCTGGAGAGGGATACCCCAGGACCTGACAC CGAAAACGGACCACAAGTACATGGGGTTACCTCACTCCTCAGGCTCCTCCTACTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218887 protein sequence
Red=Cloning site Green=Tags(s) MLKLKGELRTAKGLKNEAGERDRDVSNLNSKLLSLQLDIKNLHDVCKRQGKTLQENQLCVEEAMLKANHN KKQAQTLVFTDNQMDFRVNKQYHLRQLQQLKKKLLTLQQELEFRTQELQASYCSLLQYQSILEKQTSDLL VLHRHCKLKEDEVILYEEEMGNHSKSTGEKLHLAQEQLALAGDKILSLERSLNLYRDKYQTSLSNIELLE CQVKMLEGELSGLIGQDPENKGDHPKVRIYTSPCVIQEHQETLKRLSEVWQKVSEQDDLIQELRNKLACS NSLVLEREEALIKLQAEFASYTATHRHPPTSSEDCEDITKILKHLQEQKDSQCLHVEEYQNLVKDLRMEL EAVSEQKKKIMKDMMKLELDLHGLREETSCVIEKKDKETVFLQYRLQDLQQQYTESQKLSLKKDKLLQDK DERLHELEKNLMQVQNSLREKEAELEKLQCTTKELDTSLQEARQSTSKIDCEALRAEIQKLKDSLEEARE QLKVSDQNLTQSKEEAHLSASSLEDAHRKIENCLLQDKQKEEVIKDLQSQLHKLQKESSKIEEERKHNRQ RLQELSSELSEGQRRLSNAEKEKSLLQKTLDEDEKKIDELFHSTQVSEQKQRELTNSIRKLEEELLEIKG LLEEKREQLKKSKEQEKALEEEIEALRQEAKRKEKMAKEHLRKLDEEKENLQAELTSRSSHLDSSLNKYN SSQKVIQELNAEIARQKDSIMILQTQLDSAIQKEKNCFQNMVSKEAYEELVRKSGNCQDDLTQALEKLTQ ATSETKSLNRSLQQTQERKAQLEDEIAAYEERMKKLNMELKKLQGFQQQSEQEVHNFDKKLEEMSNQVLQ WQRQHQSDLKMLAAKESQLREFQEEMATLRESLLADEKEPYSPPAKLTPKETCRHHRENDQIMNNVEQWA KEQKIANEKLGNKLREQVKYIAKLTGEKDHLHNVMVHLQQENKKLKNEIEEKKLKAGNPRICVKAFPPNK LEPSQKGKLCCALGWRGIPQDLTPKTDHKYMGLPHSSGSSYC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019938 |
| ORF Size | 3069 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019938.1, NM_019938.2, NM_019938.3, NP_064322.1 |
| RefSeq Size | 3398 bp |
| RefSeq ORF | 3069 bp |
| Locus ID | 56523 |
| UniProt ID | Q9WVQ0 |
| MW | 119.4 kDa |
| Gene Summary | Required for normal spermatogenesis (PubMed:30032984, PubMed:30298696). It functions as a scaffold protein that attaches the sperm head-tail connecting piece to the nuclear envelope, thus maintaining sperm head and tail integrity (PubMed:30032984). May also be involved in the general organization of cellular cytoskeleton.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223237 | Pmfbp1 (untagged) - Mouse polyamine modulated factor 1 binding protein 1 (Pmfbp1), (10ug) |
CNY 13,210.00 |
|
| MG218887 | Pmfbp1 (tGFP-tagged) - Mouse polyamine modulated factor 1 binding protein 1 (Pmfbp1), (10ug) |
CNY 9,220.00 |
|
| MR218887L3 | Lenti ORF clone of Pmfbp1 (Myc-DDK-tagged) - Mouse polyamine modulated factor 1 binding protein 1 (Pmfbp1) |
CNY 10,260.00 |
|
| MR218887L4 | Lenti ORF clone of Pmfbp1 (mGFP-tagged) - Mouse polyamine modulated factor 1 binding protein 1 (Pmfbp1) |
CNY 10,260.00 |
