Kcns1 (NM_008435) Mouse Tagged ORF Clone

CAT#: MR218800

  • TrueORF®

Kcns1 (Myc-DDK-tagged) - Mouse K+ voltage-gated channel, subfamily S, 1 (Kcns1)

ORF Plasmid: DDK tGFP



  "NM_008435" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,656.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Kv9.1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR218800 representing NM_008435
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGAGCGAGTTTCCGGGTCCAGGCTCTCGGGTCCCCTGGCGGCCTAGAGACGAGGCGCTGCGCGTGA
ACGTGGGCGGAGTGCGGCGGCTGCTGAGCGCGCGCGCCCTTGCGCGCTTCCCGGGCACGCGCCTGGGCCG
CCTACAGGCGGCGGCGTCCGAGGAGCAGGCGCGGCGCCTGTGCGACGACTACGACGCAGCGGCGCACGAG
TTCTACTTTGATCGGCATCCGGGCTTCTTTCTCGGCCTCCTACACTTCTACCGCACCGGGCACCTGCACG
TCCTAGACGAGCTGTGCGTCTTCGCCTTCGGCCAGGAGGCTGACTACTGGGGCCTGGGCGAGAACGCGCT
GGCCACGTGCTGCCGCGCGCGGTATCTGGAGCGGCGTGTGGCGCGGCCTCGCGCCTGGGACGAGGACAGC
GACGCGCCGAGCAGCGTGGACCCGTGTCCCGACGAGATCTCGGACGTGCAGCGGGAGCTGGCGCGCTATG
GTGCGGCTCGCTGTGGCCGCCTGCGCCGTCGTCTCTGGCTCACCATGGAGAATCCAGGCTACTCGCTGCC
CAGCAAGCTCTTCAGCTGCGTATCCATCGGCGTGGTGCTCGCCTCCATCGCTGCCATGTGCATCCACAGC
CTGCCGGAGTACCAAGCTCGGGAGGCGGCGGCGGCGGTGGCTGCAGTGGCCGCGGGTCGCAGCGCAGAGG
AGGTGCGCGACGACCCGGTGCTGCGCCGCCTGGAGTACTTCTGCATCGCTTGGTTCAGCTTCGAGGTGTC
GTCGCGCCTGCTGCTGGCTCCCAGCACGCGCAACTTCTTCTGCCACCCGCTCAACCTCATTGACATCGTG
TCGGTGCTGCCCTTCTATCTCACACTGCTGGCTGGCGCAGCGCTTGGTGACCAGCGCGGAGCCAGCGGGG
AGGAGCTCGGGGACCTGGGCAAGGTAGTGCAAGTGTTCCGCCTCATGCGCATCTTCCGCGTGCTCAAGCT
GGCGCGCCACTCCACGGGGCTGCGTTCGCTGGGCGCCACGCTCAAGCACAGCTACCGTGAGGTGGGCATC
TTACTGCTGTACCTGGCCGTGGGTGTGTCAGTGTTCTCCGGCGTGGCCTACACAGCCGAAGAAGAAAACG
AGGGCTTTCACACAATCCCTGCCTGCTGGTGGTGGGGCACAGTGAGCATGACCACAGTGGGCTATGGGGA
TGTGGTGCCAGAGACTGTGGGTGGCAAGCTGGCGGCCTCGGGCTGCATCCTCGGGGGCATCCTGGTGGTC
GCCCTCCCCATCACCATCATCTTCAACAAGTTTTCCCACTTCTACCGGCGCCAGAAGGCACTGGAGGCGG
CCGTGCGGAGCAGCGGTCAGCGCGAGTTTGAGGACTTGCTGAGTAGCGTTGACGGGGTATCGGATGTGTC
TCTGGAAACATCCCGGGACACTTCTCAGGAGGGACGCTCTACAGACCTGGAGACCCAAGCTCCCCGGGAG
CCTGCAAAATCTCACAGTTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR218800 representing NM_008435
Red=Cloning site Green=Tags(s)

MVSEFPGPGSRVPWRPRDEALRVNVGGVRRLLSARALARFPGTRLGRLQAAASEEQARRLCDDYDAAAHE
FYFDRHPGFFLGLLHFYRTGHLHVLDELCVFAFGQEADYWGLGENALATCCRARYLERRVARPRAWDEDS
DAPSSVDPCPDEISDVQRELARYGAARCGRLRRRLWLTMENPGYSLPSKLFSCVSIGVVLASIAAMCIHS
LPEYQAREAAAAVAAVAAGRSAEEVRDDPVLRRLEYFCIAWFSFEVSSRLLLAPSTRNFFCHPLNLIDIV
SVLPFYLTLLAGAALGDQRGASGEELGDLGKVVQVFRLMRIFRVLKLARHSTGLRSLGATLKHSYREVGI
LLLYLAVGVSVFSGVAYTAEEENEGFHTIPACWWWGTVSMTTVGYGDVVPETVGGKLAASGCILGGILVV
ALPITIIFNKFSHFYRRQKALEAAVRSSGQREFEDLLSSVDGVSDVSLETSRDTSQEGRSTDLETQAPRE
PAKSHSY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_008435
ORF Size 1491 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_008435.2, NP_032461.2
RefSeq Size 2727 bp
RefSeq ORF 1494 bp
Locus ID 16538
UniProt ID O35173
MW 55.4 kDa
Gene Summary Potassium channel subunit that does not form functional channels by itself. Can form functional heterotetrameric channels with KCNB1 and KCNB2; modulates the delayed rectifier voltage-gated potassium channel activation and deactivation rates of KCNB1 and KCNB2 (PubMed:9305895).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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