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Nsmaf (NM_010945) Mouse Tagged ORF Clone
CAT＃: MR218701
- TrueORF®
Nsmaf (Myc-DDK-tagged) - Mouse neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf)
ORF Plasmid: tGFP
"NM_010945" in other vectors (4)
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CNY 6,744.00
CNY 8,170.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA959567; C630007J05; Fan |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218701 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTCACCCGGAAGAGGCAGCGCGAGCAGCAGCTGCAGCTCTACTCCAAGGAGAGGTTTTCCTTAC TGCTTCTCAACTTGGAGGAGTACTACTTTGAGCAGCACACAGCGTTCCATGTCCAGCACCAGGGCAGCCA GGAAGAAAGGAAAATCAGAGGCTCCTTAAAGATATGTTCAAAATCAGTGATTTTTGAACCAGATGCAATA TCTCAGCCCATTCTTAAGATTCCTTTGAGAGACTGTTTAAAAATAGGAAAACATGGAGAAAATGGAGCCA ATAAACACTTTGCAAAGGCAAAATCATGGGGTATTTCACTCATTTTCAGTCAGATATACTTTATTAAGGA GCACAACATTGTGGCACCGTATAAGATAGAGCGGGGAAAAATGGAGTATGTCTTTGAACTGGAAGTTTCT GGGAAGGTGGAAGATGTTGTGGAGACATTGCTACAGCTGCACAGAGCATCCTGCCTTGATAAACTTGGTG ACCAAATGGCCATGATAACAGCTATCTTGCAGTCACGTCTGGCTAGGACATCATTTGACAAAAACAGGTT CCAAAGTGTTTCTGAAAAGCTGCACATGGAGTGCAAAGCAGAGATGGTAACACCTCTAGTGACGAACCCT GGGCATGTGTGCATTACAGACACCAGCCTGTATTTCCAGCCTCTCAATGGGTACCCGAAACCTGTGGTCC AGATAACACTGCAGGATGTCCGGCGCATTTACAAAAGGAGGCACGGCCTCATGCCTTTGGGCTTGGAAGT GTTTTGCACAGATGACGACCTGTGTTCCGACATATACCTAAAGTTCTATGAGCCTCAAGACAGAGATGAT CTCTATTTTTATATCGCCACGTACCTAGAACACCACGCTGCAGAACACACTGCGGAGAGCTACATGCTGC AGTGGCAGCGAGGGCACCTTTCCAACTACCAGTACCTGCTCCACCTCAACAACCTGGCCGACCGCAGCTG CAATGACCTGTCCCAGTACCCTGTGTTTCCATGGATAATAAGTGATTACTCCAGCCCAGAGCTAGATTTG TCAAATCCTGCAACCTTCCGAGATCTTAGTAAGCCAGTGGGAGCCCTAAATGCGGAGCGACTGGAGAGAC TGCTGACTCGATACCAGGAAATGCCTGAGCCAAGATTCATGTATGGGAGTCACTACTCTTCTCCAGGCTA TGTGCTCTTTTACCTCGTTAGGATCGCACCAGAGTATATGCTGTGTCTACAAAATGGAAGATTTGATAAT GCAGATAGGATGTTTAACAGTATTGCAGAAACGTGGAAAAACTGTTTGGATGGTGCAACGGATTTTAAAG AGTTGATTCCAGAATTCTATGATGAAGACGTCAGCTTTTTGGTCAATAGTCTGAAGCTGGATCTGGGAAA GAGACAAGGCGGGCAGATGGTTGATGACGTGGACCTTCCAGCCTGGGCCTCAAGTCCCCAAGACTTCCTG CAGAAGAACAAGGATGCCTTGGAAAGCGGTTACGTGTCTGAGCACCTTCATGAGTGGATTGATCTGATAT TTGGCTATAAGCAAAAAGGGAGTGAGGCCATAGGGGCCCATAACGTGTTTCATCCCCTGACCTATGAAGG TGGTGTAGACTTGAACAGCATTGAGGATCCTGATGAAAAAGTAGCCATGCTGACCCAAATTTTGGAATTT GGGCAGACACCAAAACAGCTGTTTGTGACACCACATCCTCGAAGGATCACCCCCAAGTTTAAAAGTTTGT CCCAAGCCTCCAGCTACAACGCTTCTCTGACAGACTCCCCAGTTTCTCCAGGGGAGGAGTCGTTTGAAGA CCTGACTGAGGAAAGCAGAACTCTGGCCTGGAGTAATATCGCCAAGCTGCAGCTACATGAGCAGTACAAG ATCCACAAAGAGGCGGTTACAGGGATAGCAGTGTCTTGCAATGGCTCTTCAGTCTTTACCACCTCCCAAG ATTCCACTTTGAAGATGTTTTCTAAGGAATCCAAAATGCTACAAAGAAGTATATCATTTTCCAATATGGC TTTATCCTCTTGTTTACTGTTGCCGGGAGACACCACTGTCATAAGTTCTTCTTGGGATAACAACGTCTAT TTTTATTCCATAGCATTTGGAAGACGCCAAGATACACTGATGGGACATGACGATGCTGTCAGTAAGATCT GTTGGCATAACGACAGGCTGTACTCTGGATCTTGGGACTCTACTGTGAAGGTGTGGTCCGGTGTTCCTGC AGAAATGCCAGGAACAAAAAGACACCAGTTTGACTTACTGGCTGAGCTTGAACATGATGTCAGCGTCAAT ACTATCAATTTAAATGCTGTGAGCACACTGTTAGTTTCTGGCACCAAAGAAGGCATGGTGAATATTTGGG ACCTCACTACAGCCACTTTATTACACCAGACTTCATGCCATTCAGGGACAGTATGTGATGCTGCTTTTAG CCCAGATAGTCGCCACATCCTCAGCACGGGCGTAGATGGATGTCTCAATGTGATTGATGTGCAGACGGGC ATGCTCATCTCCTCCATGGCCTCAGAGGAGCCGCAGAGGTGCTTTGTCTGGGATGGAAATTCTGTCTTAT CTGGAAGTCGGTCTGGTGAGCTGCTTGTTTGGGACCTCCTTGGAGCAAAAGTCAGTGAGCGGATTCAGGG CCACACTGGTGCTGTGACATGCATGTGGATGAACGAACAGTGTAGCAGTATTATCACCGGAGGGGAAGAC AGACAAATTATGTTCTGGAAATTGCAGTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218701 protein sequence
Red=Cloning site Green=Tags(s) MAFTRKRQREQQLQLYSKERFSLLLLNLEEYYFEQHTAFHVQHQGSQEERKIRGSLKICSKSVIFEPDAI SQPILKIPLRDCLKIGKHGENGANKHFAKAKSWGISLIFSQIYFIKEHNIVAPYKIERGKMEYVFELEVS GKVEDVVETLLQLHRASCLDKLGDQMAMITAILQSRLARTSFDKNRFQSVSEKLHMECKAEMVTPLVTNP GHVCITDTSLYFQPLNGYPKPVVQITLQDVRRIYKRRHGLMPLGLEVFCTDDDLCSDIYLKFYEPQDRDD LYFYIATYLEHHAAEHTAESYMLQWQRGHLSNYQYLLHLNNLADRSCNDLSQYPVFPWIISDYSSPELDL SNPATFRDLSKPVGALNAERLERLLTRYQEMPEPRFMYGSHYSSPGYVLFYLVRIAPEYMLCLQNGRFDN ADRMFNSIAETWKNCLDGATDFKELIPEFYDEDVSFLVNSLKLDLGKRQGGQMVDDVDLPAWASSPQDFL QKNKDALESGYVSEHLHEWIDLIFGYKQKGSEAIGAHNVFHPLTYEGGVDLNSIEDPDEKVAMLTQILEF GQTPKQLFVTPHPRRITPKFKSLSQASSYNASLTDSPVSPGEESFEDLTEESRTLAWSNIAKLQLHEQYK IHKEAVTGIAVSCNGSSVFTTSQDSTLKMFSKESKMLQRSISFSNMALSSCLLLPGDTTVISSSWDNNVY FYSIAFGRRQDTLMGHDDAVSKICWHNDRLYSGSWDSTVKVWSGVPAEMPGTKRHQFDLLAELEHDVSVN TINLNAVSTLLVSGTKEGMVNIWDLTTATLLHQTSCHSGTVCDAAFSPDSRHILSTGVDGCLNVIDVQTG MLISSMASEEPQRCFVWDGNSVLSGSRSGELLVWDLLGAKVSERIQGHTGAVTCMWMNEQCSSIITGGED RQIMFWKLQY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010945 |
| ORF Size | 2763 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010945.2, NP_035075.2 |
| RefSeq Size | 3518 bp |
| RefSeq ORF | 2763 bp |
| Locus ID | 18201 |
| UniProt ID | O35242 |
| MW | 104.5 kDa |
| Gene Summary | Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N-smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222630 | Nsmaf (untagged) - Mouse neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), (10ug) |
CNY 11,880.00 |
|
| MG218701 | Nsmaf (tGFP-tagged) - Mouse neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), (10ug) |
CNY 9,030.00 |
|
| MR218701L3 | Lenti ORF clone of Nsmaf (Myc-DDK-tagged) - Mouse neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf) |
CNY 10,070.00 |
|
| MR218701L4 | Lenti ORF clone of Nsmaf (mGFP-tagged) - Mouse neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf) |
CNY 10,070.00 |
