United States
Germany
Japan
United Kingdom
China
Pofut2 (NM_030262) Mouse Tagged ORF Clone
CAT＃: MR218637
- TrueORF®
Pofut2 (Myc-DDK-tagged) - Mouse protein O-fucosyltransferase 2 (Pofut2)
ORF Plasmid: tGFP
"NM_030262" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310011G23Rik; AI256847; BC003494; C21orf80; FUT13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218637 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCTCAGCGTCGTCTGCCTGCTGCTGGCAGCGGCATCCTGGCGGCCGGTCTCGGCTTCAGGAG AAGAATTCTGGCCTGGCCAGTCGGCGGCGGACATCCTGTCGGGGGCGGCATCCCGCAGACGGTACCTCCT GTATGATGTCAACCCCCCAGAAGGCTTCAATCTCCGCAGGGATGTCTATATCCGGGTGGCATCCTTGCTG AAGACGCTGTTGAAGACTGAAGAGTGGGTGCTAGTCCTGCCCCCGTGGGGCCGCCTCTACCACTGGCAAA GCCCTGACATCCACCAGGTCCGGATTCCCTGGTCCGAGTTTTTTGACCTCCCGAGTCTCAATAAAAACAT CCCCGTTATAGAGTATGAGCAGTTCATTGCAGAGTCCGGCGGGCCCTTTATCGACCAGGTGTACGTCCTA CAAGGTTACGCCGAGGGCTGGAAGGAGGGCACCTGGGAGGAGAAGGTGGACGCACGGCCCTGTATTGACC CTCTGCTGTACTCGCAGGACAAGCATGAGTATTACAGAGGCTGGTTTTGGGGCTACGAAGAAACACGGGG TCTGAATGTCTCCTGCCTGTCCGTCCAGGGGTCGGCTTCCATCGTCGCGCCTGTCCTCTTAAAAAACACA TCTGCTCGGTCTGTGATGCTGGACCGAGCTGAGAATCTGCTTCATGACCACTATGGAGGCCGGGAGTACT GGGATACCCGGCGCAGCATGGTGTTTGCAAAGCACTTGCGGGCTGTGGGAGACGAGTTTAGGAGCCAGCA CCTCAACTCGACGGATGCCGCTGATAAGATGGCCCCGGAGGAAGACTGGACGAAGATGAAGGTCAAACTG GGCTCAGCACTAGGCGGCCCCTACCTCGGAGTCCACCTGAGAAGGAAGGACTTCATCTGGGGCCACAGGG AGGATGTGCCCAGCCTGGAGGGCGCCGTGAAGAAGATCCGCAGCCTCATGAAGACTCATCAGCTGGACAA GGTGTTCGTGGCCACAGACGCCATCAGGAAGGAGCAGGAAGAATTGAGGAAGCTGCTGCCGGAAATGGTG AGGTTTGAGCCCACGTGGGAGGAGCTGGAGCTGTACAAGGACGGAGGCGTCGCCATCATCGATCAGTGGA TCTGCGCTCATGCCAGGTTTTTTATTGGCACCTCTGTTTCCACATTTTCCTTTCGGATTCATGAAGAGAG AGAGATCCTGGGGTTGGACCCCAAGACAACATACAACCGGTTTTGTGGAGACCAGGAGAAAGCATGTGAG CAGCCCACACACTGGAAGATTGCGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218637 protein sequence
Red=Cloning site Green=Tags(s) MAALSVVCLLLAAASWRPVSASGEEFWPGQSAADILSGAASRRRYLLYDVNPPEGFNLRRDVYIRVASLL KTLLKTEEWVLVLPPWGRLYHWQSPDIHQVRIPWSEFFDLPSLNKNIPVIEYEQFIAESGGPFIDQVYVL QGYAEGWKEGTWEEKVDARPCIDPLLYSQDKHEYYRGWFWGYEETRGLNVSCLSVQGSASIVAPVLLKNT SARSVMLDRAENLLHDHYGGREYWDTRRSMVFAKHLRAVGDEFRSQHLNSTDAADKMAPEEDWTKMKVKL GSALGGPYLGVHLRRKDFIWGHREDVPSLEGAVKKIRSLMKTHQLDKVFVATDAIRKEQEELRKLLPEMV RFEPTWEELELYKDGGVAIIDQWICAHARFFIGTSVSTFSFRIHEEREILGLDPKTTYNRFCGDQEKACE QPTHWKIAY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030262 |
| ORF Size | 1290 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030262.3, NP_084538.2 |
| RefSeq Size | 2219 bp |
| RefSeq ORF | 1290 bp |
| Locus ID | 80294 |
| UniProt ID | Q8VHI3 |
| MW | 49.4 kDa |
| Gene Summary | Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively. O-fucosylates members of several protein families including the ADAMTS superfamily and the thrombosporin (TSP) and spondin families. Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13 (By similarity). O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211921 | Pofut2 (untagged) - Mouse protein O-fucosyltransferase 2 (Pofut2), (10ug) |
CNY 3,990.00 |
|
| MG218637 | Pofut2 (tGFP-tagged) - Mouse protein O-fucosyltransferase 2 (Pofut2), (10ug) |
CNY 3,140.00 |
|
| MR218637L3 | Lenti ORF clone of Pofut2 (Myc-DDK-tagged) - Mouse protein O-fucosyltransferase 2 (Pofut2) |
CNY 4,750.00 |
|
| MR218637L4 | Lenti ORF clone of Pofut2 (mGFP-tagged) - Mouse protein O-fucosyltransferase 2 (Pofut2) |
CNY 4,750.00 |
