Amacr (NM_008537) Mouse Tagged ORF Clone
CAT#: MR218634
- TrueORF®
Amacr (Myc-DDK-tagged) - Mouse alpha-methylacyl-CoA racemase (Amacr), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_008537" in other vectors (3)
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CNY 3,800.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Macr1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR218634 representing NM_008537
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTGCGTGGCGTCAGGGTTGTGGAGCTGGCAGGCCTGGCCCCGGGGCCGTTCTGCGGAATGGTCC TGGCGGACTTCGGCGCCGAGGTGGTGCGCGTGAACCGGCTGGGCTCCACGGGCGAGAATTTTCTGGCCCG AGGCAAGCGCTCGCTAGCGCTGGACCTGAAGCGCTCTCAGGGAGTCACGGTGTTGCGGCGCATGTGCGCA CGCGCGGACGTGTTGCTGGAGCCCTTCCGCTGCGGTGTCATGGAGAAACTCCAGCTTGGGCCAGAGACTC TACTGCAGGACAATCCAAAGCTCATCTATGCCAGGCTGAGCGGATTTGGCCAATCGGGAATTTTCTCCAA AGTAGCTGGCCATGACATCAACTATTTGGCTTTATCAGGCGTTCTGTCAAAGATTGGCAGAAGCGGTGAG AACCCCTACCCACCGCTGAATCTCCTGGCTGACTTTGGCGGTGGAGGCCTCATGTGCACACTGGGCATTG TGCTGGCTCTCTTTGAACGCACACGCTCTGGCCGAGGGCAGGTCATCGATTCAAGCATGGTGGAAGGGAC TGCATACTTAAGTTCTTTCCTGTGGAAAACCCAGCCCATGGGTCTGTGGAAACAGCCTCGAGGACAAAAC ATCTTAGATGGCGGTGCACCTTTCTACACAACCTACAAGACGGCAGACGGGGAGTTCATGGCTGTAGGTG CCATAGAACCCCAGTTCTATGCACTGCTGCTTAAAGGACTTGGACTCGAGTCTGAGGAACTCCCCTCCCA GATGAGCTCAGCAGATTGGCCAGAGATGAAGAAGAAATTTGCAGATGTGTTTGCAAAGAAGACTAAGGCA GAATGGTGCCAGATCTTTGACGGGACAGATGCGTGTGTGACCCCAGTGCTGACGTTTGAGGAGGCCCTCC ACCACCAGCACAACAGAGAACGGGCCTCCTTCATCACTGATGGGGAGCAGCTCCCGAGCCCCCGCCCTGC ACCTCTGCTTTCCAGAACTCCTGCCGTCCCATCTGCCAAAAGGGACCCTTCTGTAGGGGAGCACACCGTA GAAGTGCTTAGAGAGTATGGATTCAGTCAGGAAGAGATCCTTCAGCTGCACTCAGATAGAATCGTTGAAA GTGATAAGCTAAAAGCCAATCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218634 representing NM_008537
Red=Cloning site Green=Tags(s) MVLRGVRVVELAGLAPGPFCGMVLADFGAEVVRVNRLGSTGENFLARGKRSLALDLKRSQGVTVLRRMCA RADVLLEPFRCGVMEKLQLGPETLLQDNPKLIYARLSGFGQSGIFSKVAGHDINYLALSGVLSKIGRSGE NPYPPLNLLADFGGGGLMCTLGIVLALFERTRSGRGQVIDSSMVEGTAYLSSFLWKTQPMGLWKQPRGQN ILDGGAPFYTTYKTADGEFMAVGAIEPQFYALLLKGLGLESEELPSQMSSADWPEMKKKFADVFAKKTKA EWCQIFDGTDACVTPVLTFEEALHHQHNRERASFITDGEQLPSPRPAPLLSRTPAVPSAKRDPSVGEHTV EVLREYGFSQEEILQLHSDRIVESDKLKANL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_008537 |
ORF Size | 1143 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_008537.4, NP_032563.2 |
RefSeq Size | 2575 bp |
RefSeq ORF | 1146 bp |
Locus ID | 17117 |
UniProt ID | O09174 |
MW | 42.2 kDa |
Gene Summary | Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (By similarity). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG218634 | Amacr (tGFP-tagged) - Mouse alpha-methylacyl-CoA racemase (Amacr) nuclear gene encoding mitochondrial protein, (10ug) |
CNY 4,180.00 |
|
MR218634L3 | Lenti ORF clone of Amacr (Myc-DDK-tagged) - Mouse alpha-methylacyl-CoA racemase (Amacr), nuclear gene encoding mitochondrial protein |
CNY 5,700.00 |
|
MR218634L4 | Lenti ORF clone of Amacr (mGFP-tagged) - Mouse alpha-methylacyl-CoA racemase (Amacr), nuclear gene encoding mitochondrial protein |
CNY 5,700.00 |