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Pram1 (NM_001002842) Mouse Tagged ORF Clone
CAT＃: MR218463
- TrueORF®
Pram1 (Myc-DDK-tagged) - Mouse PML-RAR alpha-regulated adaptor molecule 1 (Pram1), transcript variant 1
ORF Plasmid: tGFP
"NM_001002842" in other vectors (4)
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CNY 4,944.00
CNY 5,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AY665714 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218463 representing NM_001002842
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGCAATCAGGACTTCCGAAACCTCCAAGCTAAGTTCCAAACCTCTCAGCCAGAACTTGGAGAAC TGTTCAGAAAAACCCCAAAGCCTGAGTTAAACAAAGTCCTGAAGAAATTTCCACAGACTGAGTTAAGTGA GCAGCCCAAGAAGTCCTCACAGTCTGAGCTCAGTGCAGTGTCTTTGAAACCACTGCAGCTGCAGTTTGCA GACCTCCCCAGGAAGCCCCCACAGCCTGGAGTTCTTAAAAAGTCACCACAGCCTGAGTTCCCTCATCTAG CCAACAAGCCTGTACAGGCTGAATTCCCAAGGAAGCCTCTACATCCAGAGTTCACAGGTCTCAAAAAGCC TTCCCAGGCTGAGTTCACAGATCTCAAGAAGCCCCCACAGCCCCAGTTTGCCAGCCTCCCCAAGAAGCCC CCAAAACCTGAGTTTGGAGAGCTCTCCAAGAGACCTCCACAGCTTGAGACACCCCAGGAGCCTAGTGCTC CTCCTGCCCAGAAGCTCCTGAAGCCCGAGCCTAACAATCCTGCCAGGCCTCTAGGAGAGCTCAAACCCAA GATGTTTTGGCACCTTGAGGCCAATGAGGCACCTAAGAGGCCCTTGCCATCTGAATCCAGTACTTTTCCC AAGAAGCCACTGCAGCCTGAGGCTGTTGTTGGTTTCTCTAGGAAGTCCCAGCCACAGTCAGAGTCCATCG AAGTTTCTCAGACATCCCCCTCTAAGTGTGGGTCCAGGGAGCTTGACTCTCACTCCCCACAGCCTGACAT CAGTACCTTTCCTAAGAACAATGAGAACTTTAGGAAACCTTCATATCCTCAAGCCACTGGTTGCCCCAAG TCCCCAAAGCAGCCCATGTTCTATGAGTTCCCCCAGACACCCCCTCGGAAGCCTGAGTCATGTAACCCCC AGTCTCACTCCCCGCTGCCAGACTTCAATGCATTCCCTAAGAAGCATCCACAGCTCCAGCCAAGTGACCT TACCAGGGCATCCTCGGAGCCTGAAGTCTGCAAGGTTCCCAAAAAGACTCAGAAGCCAGACCCCAATGTG CTTTCTCAGAAGCCCTCACAGCCAGAATTGGGTCACCTCCCTAGAACATCCTCGGACCCCGAGTTCAACT CGCTCCCGAGGAAGTTTCTGCAGCCTCAACACGGCAAGTTCTTCCAGCCTGAGTTCCCCAAGGGTCTGCC TAGAAAGCCCAAGCTTCCTGGTTCAGTGTCTGAATGCTCCCTGCCCTCTGCTAGTGCGGGCTCCAGCCCC CAGTGCCCTCTCAGCCCTGGGCTTATAGTCCCTGGGATACCCCGCTGGAGATCAGAAGACTTCCAAGTCC AGCGCCCACCCCGACGGCGGCCCCTGCCCTCAGCCAGCAGCCTGGGACACCCCCCAGCCAAGCCCGCACT ACCACCTGGCCCCATCAATATCCAAAGCTTCAGGAGAGCCGCAGCAACAGCCGCAGCGGTACTGAAGACA GGCTCTTCTACTGGGACCCACTTCCAAGCTCAACAACCTCAACATATTGCACAGAACCCGGATGAGATCT ACGAACTGTATGATGCTGTGGAGGCCACAGATGACTCTAGTATCAGCCCCAGAGGCAGAGATGAAATGCT GTCTACCCAGCAAGCCACCAGATGGCCTCAACAGGAGCCAGAGCTCAGGAAGAAGGCCACTCAGCCACAG CAGCTGCCAGCTACAGACCCCAAGCTGCTGAAACAGATCAGGAAGGCAGAAAAGGCTGAGAGAGAGTTTA GAAAGAAGTTTAAGTTTGAAGGTGAGATTGTGATTCACACGAAGATGATGATTGACCCCAATGCCAAGAC CCGGCGTGGGGGTGGCAAGCACCTGGGCATCCGGCGTGGAGAGATCCTAGAGGTGATCGAGTTCACTAGC AAGGATGAGATGCTCTGCCGGGACCCCAAGGGCAAGTATGGCTATGTGCCCAGAACTGCACTGTTGCCCT TGGAAACAGAGGTATATGATGACGTCAGCTTTGGGGACCCTCTGGACATGCAACCATTTCCTCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218463 representing NM_001002842
Red=Cloning site Green=Tags(s) MGSNQDFRNLQAKFQTSQPELGELFRKTPKPELNKVLKKFPQTELSEQPKKSSQSELSAVSLKPLQLQFA DLPRKPPQPGVLKKSPQPEFPHLANKPVQAEFPRKPLHPEFTGLKKPSQAEFTDLKKPPQPQFASLPKKP PKPEFGELSKRPPQLETPQEPSAPPAQKLLKPEPNNPARPLGELKPKMFWHLEANEAPKRPLPSESSTFP KKPLQPEAVVGFSRKSQPQSESIEVSQTSPSKCGSRELDSHSPQPDISTFPKNNENFRKPSYPQATGCPK SPKQPMFYEFPQTPPRKPESCNPQSHSPLPDFNAFPKKHPQLQPSDLTRASSEPEVCKVPKKTQKPDPNV LSQKPSQPELGHLPRTSSDPEFNSLPRKFLQPQHGKFFQPEFPKGLPRKPKLPGSVSECSLPSASAGSSP QCPLSPGLIVPGIPRWRSEDFQVQRPPRRRPLPSASSLGHPPAKPALPPGPINIQSFRRAAATAAAVLKT GSSTGTHFQAQQPQHIAQNPDEIYELYDAVEATDDSSISPRGRDEMLSTQQATRWPQQEPELRKKATQPQ QLPATDPKLLKQIRKAEKAEREFRKKFKFEGEIVIHTKMMIDPNAKTRRGGGKHLGIRRGEILEVIEFTS KDEMLCRDPKGKYGYVPRTALLPLETEVYDDVSFGDPLDMQPFPR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001002842 |
| ORF Size | 2025 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001002842.2, NP_001002842.2 |
| RefSeq Size | 2290 bp |
| RefSeq ORF | 2028 bp |
| Locus ID | 378460 |
| UniProt ID | Q6BCL1 |
| MW | 75.5 kDa |
| Gene Summary | May be involved in integrin signaling in neutrophils. Binds to PtdIns(4)P.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220391 | Pram1 (untagged) - Mouse PML-RAR alpha-regulated adaptor molecule 1 (Pram1), transcript variant 1, (10ug) |
CNY 8,650.00 |
|
| MG218463 | Pram1 (tGFP-tagged) - Mouse PML-RAR alpha-regulated adaptor molecule 1 (Pram1) transcript variant 1, (10ug) |
CNY 6,560.00 |
|
| MR218463L3 | Lenti ORF clone of Pram1 (Myc-DDK-tagged) - Mouse PML-RAR alpha-regulated adaptor molecule 1 (Pram1), transcript variant 1 |
CNY 7,890.00 |
|
| MR218463L4 | Lenti ORF clone of Pram1 (mGFP-tagged) - Mouse PML-RAR alpha-regulated adaptor molecule 1 (Pram1), transcript variant 1 |
CNY 7,890.00 |
