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Lpcat2 (NM_173014) Mouse Tagged ORF Clone
CAT＃: MR218203
- TrueORF®
Lpcat2 (Myc-DDK-tagged) - Mouse lysophosphatidylcholine acyltransferase 2 (Lpcat2)
ORF Plasmid: tGFP
"NM_173014" in other vectors (4)
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CNY 6,072.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A330042H22; Aytl1; Aytl1a; lpafat1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218203 representing NM_173014
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCGATGCGCCGAGGCGGCCGCTGTGGCGGCTACGGTCCCGGGTTCGGGCGTCGGGGACGCCGGGC TGCGGCCACCCATGGTACCTCGCCAGGCGTCCTTCTTCCCGCCGCCGGTGCCCAACCCCTTCGTCCAGCA GACTACGATCAGTGCCTCCCGAAGGCTGCAGATGTTTCTTCTTGGAATTATTCTGCTCCCAGTCCGTGCT TTACTGGTTGGAATAATTTTGCTGCTCGCATGGCCATTTGCTGTCATTTCAACAGCATGCTGTCCTGAAA AGCTGACCCATCCAATAAGCAATTGGAGGAGGAAGATCACTCGGCCCGCTTTGACATTTCTGGCGCGTGC CATGTTCTTCTCCATGGGGTTTACGGTTACCGTGAAAGGAAAGGTTGCAAGCCCTCTGGAAGCGCCCATT TTTGTCGTCGCTCCTCATTCCACGTTCTTCGACGGAATTGCCTGTGTAGTAGCTGGGCTGCCTTCGCTGG TATCACGGAATGAGAATGCACAGACCCCTCTGGTTGGCAGACTGTTACGGGCTTTGCAGCCAGTTTTGGT ATCCCGTGTTGACCCTGACTCCCGAAAAAACACAATCAATGAAATCAAAAAGCGAGCGACATCAGGTGGA GAATGGCCCCAGATACTAGTTTTCCCAGAAGGTACTTGTACTAATCGCTCCTGTTTGATTACTTTTAAAC CAGGAGCCTTCATCCCAGGAGTTCCAGTGCAGCCCGTCCTCCTCAGATACCCAAACAAGTTGGATACTGT GACCTGGACCTGGCAAGGCTATACATTCCTCCAGCTGTGTGTGCTCACTTTCTGTCAACTCTTCACGAAG GTGGAGATTGAGTTTATGCCTGTGCAAGCACCCAGTGAGGAAGAGAAGAACGATCCTGTCCTTTTCGCCA GTAGAATCCGGAACTTAATGGCTGAAGCATTGGAAATACCCGTGACGGATCACACCTACGAAGACTGCAG GCTGATGATCTCAGCGGGACAGCTCACGTTGCCTATGGAGGCCGGGCTGGTAGAATTTTCAAAGATTAGC CGGAAACTGAAATTAGACTGGGATGGTATCCGGAAGCACTTGGATGAATATGCGTCTATCGCAAGCTCTT CCAAAGGAGGCAGAATTGGAATCGAGGAGTTTGCGGAATACTTGAAGCTACCTGTTTCCGATGTCCTGAG ACAACTTTTTGCACTCTTTGACAGGAATAATGATGGCAGTATTGACTTCCGAGAGTATGTGATCGGCCTG GCTGTCTTGTGCAACCCTGCCAATACAGAAGAGATCATCCAGGTGGCATTTAAGCTCTTTGATGTTGATG AGGACGGCTACATAACAGAGGAGGAGTTTTGCACCATTCTGCAGGCTTCTCTTGGAGTGCCTGACCTTAA TGTTTCTGGTCTCTTCAGAGAAATTGCCCAACGGGACTCTGTTTCCTACGAGGAATTCAAAAGTTTCGCC CTAAAGCACCCAGAATATGCCAAGATATTTACCACATACCTAGACCTCCAGACATGCCATGTATTCTCCT TGCCAGAAGAGGTCCAGACAGCTCCCTCTGTTGCTAGTAATAAAGTCAGTCCTGAGAGCCAGGAGGAGGG CACCTCAGACAAAAAGGTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218203 representing NM_173014
Red=Cloning site Green=Tags(s) MNRCAEAAAVAATVPGSGVGDAGLRPPMVPRQASFFPPPVPNPFVQQTTISASRRLQMFLLGIILLPVRA LLVGIILLLAWPFAVISTACCPEKLTHPISNWRRKITRPALTFLARAMFFSMGFTVTVKGKVASPLEAPI FVVAPHSTFFDGIACVVAGLPSLVSRNENAQTPLVGRLLRALQPVLVSRVDPDSRKNTINEIKKRATSGG EWPQILVFPEGTCTNRSCLITFKPGAFIPGVPVQPVLLRYPNKLDTVTWTWQGYTFLQLCVLTFCQLFTK VEIEFMPVQAPSEEEKNDPVLFASRIRNLMAEALEIPVTDHTYEDCRLMISAGQLTLPMEAGLVEFSKIS RKLKLDWDGIRKHLDEYASIASSSKGGRIGIEEFAEYLKLPVSDVLRQLFALFDRNNDGSIDFREYVIGL AVLCNPANTEEIIQVAFKLFDVDEDGYITEEEFCTILQASLGVPDLNVSGLFREIAQRDSVSYEEFKSFA LKHPEYAKIFTTYLDLQTCHVFSLPEEVQTAPSVASNKVSPESQEEGTSDKKVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_173014 |
| ORF Size | 1632 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_173014.2 |
| RefSeq Size | 2800 bp |
| RefSeq ORF | 1635 bp |
| Locus ID | 270084 |
| UniProt ID | Q8BYI6 |
| MW | 60.7 kDa |
| Gene Summary | Possesses both acyltransferase and acetyltransferase activities (PubMed:17182612, PubMed:18156367). Activity is calcium-dependent (PubMed:17182612). Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF) (PubMed:17182612). Also converts lyso-PAF to 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (PC), a major component of cell membranes and a PAF precursor (PubMed:17182612, PubMed:18156367). Under resting conditions, acyltransferase activity is preferred (PubMed:17182612). Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases (PubMed:17182612). Also catalyzes the conversion of 1-acyl-sn-glycero-3-phosphocholine to 1,2-diacyl-sn-glycero-3-phosphocholine. Involved in the regulation of lipid droplet number and size (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Lysophosphatidylcholine acyltransferase 2 (LPCAT2) co-localises with TLR4 and regulates macrophage inflammatory gene expression in response to LPS
,Abate, W;Alrammah, H;Kiernan, M;Tonks, AJ;Jackson, SK;,
Sci Rep
,PubMed ID 32587324
[LPCAT2]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218670 | Lpcat2 (untagged) - Mouse lysophosphatidylcholine acyltransferase 2 (Lpcat2), (10ug) |
CNY 6,940.00 |
|
| MG218203 | Lpcat2 (tGFP-tagged) - Mouse lysophosphatidylcholine acyltransferase 2 (Lpcat2), (10ug) |
CNY 5,990.00 |
|
| MR218203L3 | Lenti ORF clone of Lpcat2 (Myc-DDK-tagged) - Mouse lysophosphatidylcholine acyltransferase 2 (Lpcat2) |
CNY 7,320.00 |
|
| MR218203L4 | Lenti ORF clone of Lpcat2 (mGFP-tagged) - Mouse lysophosphatidylcholine acyltransferase 2 (Lpcat2) |
CNY 7,320.00 |
