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Acer3 (NM_025408) Mouse Tagged ORF Clone
CAT＃: MR218055
- TrueORF®
Acer3 (Myc-DDK-tagged) - Mouse alkaline ceramidase 3 (Acer3)
ORF Plasmid: tGFP
"NM_025408" in other vectors (4)
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CNY 2,400.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110057L18Rik; 5430429L08Rik; AV015045; Phca |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218055 representing NM_025408
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCGGCTGTGGACCGCAAAGGCTATTGGGGCCCCACGACCTCCACATTGGACTGGTGTGAGGAGA ACTATGTGGTGACCTTGTTCGTCGCTGAGTTCTGGAATACAGTGAGTAACCTGATTATGATCATACCTCC AATTTTTGGTGCAATTCAAGGCATTAGAGACAGACTGGAGAAGCGGTACATTGCTGCTTACTTAGCACTC ACAGTGGTAGGAATGGGATCCTGGTGTTTCCACATGACTCTGAAATATGAAATGCAGCTGTTGGATGAGC TCCCCATGATTTACAGCTGCTGCATATTTGTATACTGCATGTTTGAGTGTTTCAAGACAAAGAGCTCAAT AAACTACCATCTTCTTTTTACCCTATTTCTATACAGTTTAACAGTAACTACGATTTACCTAAAAGTCAAA GAACCTATATTCCATCAGGTCATGTATGGAATGTTGGTCTTTACATTAGTACTTCGTTCTATTTATATTG TTACATGGGTTTATCCATGGCTTAGAGGACTAGGTTATACATCCTTAACTGTCTTTTTATTGGGGTTTTT ATTGTGGAATATAGATAACATCTTTTGTGATTCACTGAGGAACTTTCGAAAGAGAGTGCCCCCCGTCCTA GGTGTTACAACACAGTTTCATGCATGGTGGCATATTCTAACTGGCCTGGGTTCTTATCTTCACATCCTTT TCAGTTTATATACAAGAACACTTTACCTGAGGTACAGGCCAAAAGTGAAGTTTCTCTTTGGAATCTGGCC AGCAGTCATGTTTGAACCTCAGAGGAAGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218055 representing NM_025408
Red=Cloning site Green=Tags(s) MAPAVDRKGYWGPTTSTLDWCEENYVVTLFVAEFWNTVSNLIMIIPPIFGAIQGIRDRLEKRYIAAYLAL TVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKTKSSINYHLLFTLFLYSLTVTTIYLKVK EPIFHQVMYGMLVFTLVLRSIYIVTWVYPWLRGLGYTSLTVFLLGFLLWNIDNIFCDSLRNFRKRVPPVL GVTTQFHAWWHILTGLGSYLHILFSLYTRTLYLRYRPKVKFLFGIWPAVMFEPQRKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025408 |
| ORF Size | 801 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025408.3 |
| RefSeq Size | 4058 bp |
| RefSeq ORF | 804 bp |
| Locus ID | 66190 |
| UniProt ID | Q9D099 |
| MW | 32 kDa |
| Gene Summary | Endoplasmic reticulum and Golgi ceramidase that catalyzes the hydrolysis of unsaturated long-chain C18:1-, C20:1- and C20:4-ceramides, dihydroceramides and phytoceramides into sphingoid bases like sphingosine and free fatty acids at alkaline pH (PubMed:26474409). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:26474409). Controls the generation of sphingosine in erythrocytes, and thereby sphingosine-1-phosphate in plasma (By similarity). Through the regulation of ceramides and sphingosine-1-phosphate homeostasis in the brain may play a role in neurons survival and function (PubMed:26474409). By regulating the levels of proinflammatory ceramides in immune cells and tissues, may modulate the inflammatory response (PubMed:26938296).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
New fluorogenic probes for neutral and alkaline ceramidases [S]
,null,
Journal of Lipid Research
,PubMed ID 30926626
[Acer3]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210295 | Acer3 (untagged) - Mouse alkaline ceramidase 3 (Acer3), (10ug) |
CNY 3,990.00 |
|
| MG218055 | Acer3 (tGFP-tagged) - Mouse alkaline ceramidase 3 (Acer3), (10ug) |
CNY 2,850.00 |
|
| MR218055L3 | Lenti ORF clone of Acer3 (Myc-DDK-tagged) - Mouse alkaline ceramidase 3 (Acer3) |
CNY 4,750.00 |
|
| MR218055L4 | Lenti ORF clone of Acer3 (mGFP-tagged) - Mouse alkaline ceramidase 3 (Acer3) |
CNY 4,800.00 |
