Fem1c (NM_173423) Mouse Tagged ORF Clone

CAT#: MR218038

  • TrueORF®

Fem1c (Myc-DDK-tagged) - Mouse fem-1 homolog c (C.elegans) (Fem1c)

ORF Plasmid: DDK tGFP



  "NM_173423" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,592.00

CNY 5,420.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Goat Polyclonal Antibody against FEM1C
    • 100 ug

CNY 5,371.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2610312A07Rik; 3632443A22Rik; AI646567
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR218038 representing NM_173423
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCTAAAGACAGCGGTGTTCAACGCAGCGCGCGATGGCAAACTCCGGCTCCTCACCAAGTTGCTGG
CAAGCAAATCGAAAGCGGAGGTTTCCTCCTTAATCTCGGAGAAAACAAATGGGGCCACCCCACTCTTGAT
GGCTGCTAGGTATGGACACCTCGACATGGTAGAATTCCTTCTAGAGCAGTGCAGTGCTTCCATAGAAGTC
GGGGGCTCTGTTAACTTTGACGGGGAAACCATCGAGGGGGCCCCCCCTTTATGGGCCGCTTCTGCAGCAG
GACATCTGAAGGTTGTCCAGTCTTTGTTAAATCACGGAGCATCTGTCAACAACACGACTTTAACCAACTC
AACTCCTCTTCGAGCCGCGTGCTTCGATGGCCATTTGGAAATAGTGAAATACCTTGTAGAACACAAAGCC
GATTTGGAAGTGTCCAACCGCCATGGGCATACTTGCTTGATGATTTCATGCTATAAAGGACACAAAGAAA
TCGCTCAGTATTTACTTGAAAAGGGGGCAGATGTTAACAGAAAAAGTGTTAAAGGCAACACTGCATTGCA
TGATTGTGCAGAGTCTGGAAGTTTGGACATCATGAAGATGCTGCTTATGTATTGTGCCAAGATGGAGAAA
GATGGCTACGGAATGACTCCTCTTCTCTCAGCAAGTGTGACTGGTCACACTAATATTGTGGATTTTCTTA
CGCACCATGCCCAGACAAGCAAGACAGAACGTATTAATGCTCTAGAGCTTCTGGGAGCTACGTTTGTAGA
TAAAAAAAGAGATTTACTTGGGGCTTTGAAATACTGGAAAAAGGCGATGAACATGAGGTACAGTGATAGG
ACTAATATAATTAGTAAACCAGTCCCACAGACACTGATAATGGCCTATGACTATGCCAAGGAGGTAAACA
GTGCAGAAGAGTTAGAAGGTCTTATTGCTGATCCTGATGAGATGAGAATGCAAGCGCTGTTAATTAGAGA
ACGGATTCTTGGTCCTTCTCATCCCGATACCTCTTACTACATTAGATATAGGGGTGCTGTCTACGCTGAC
TCTGGAAATTTTAAGCGATGCATCAACCTATGGAAATATGCTTTGGATATGCAGCAGAGCAATTTGGACC
CTTTAAGCCCAATGACTGCCAGCAGCTTACTGTCTTTTGCAGAACTGTTCTCCTTCATGCTACAGGACAG
GGCTAAAGGCTTGCTGGGCACTACTGTTACATTTGATGATCTCATGGGCATACTGTGCAAAAGTGTCCTT
GAAATTGAGCGAGCTATCAAACAAACTCAGTGTCCAGCTGACCCATTGCAGTTAAATAAGGCTCTTTCCA
TCATTTTGCACTTAATTTGCTTATTAGAAAAAGTTCCTTGTACTGTAGAACAGGATCATTTCAAAAAGCA
GACCATCTACAGGTTTCTTAAGTTGCATCCAAGAGGAAAGAATAACTTCAGCCCTCTGCATCTGGCTGTG
GACAAGAATACTACATGTGTGGGGCGGTACCCTGTTTGTAAGTTTCCATCTCTGCAAGTTACTGCGATAC
TGATAGAATGTGGTGCTGATGTGAATGTCAGAGACTCTGATGACAACAGTCCCCTACATATCGCGGCTCT
GAATAACCATCCAGACATCATGAATCTCCTTATTAAATCAGGTGCACATTTTGATGCCACAAACTTACAC
AAACAGACTGCCAGTGACTTGCTGGACGAGAAGGAGATAGCTAAAAATTTGATACAGCCCATAAATCATA
CCACATTGCAGTGTCTTGCTGCTCGTGTCATCGTGAATCATAGAATCTATTACAAAGGGAACATCCCAGA
AAAGCTAGAGACCTTTGTTTCACTTCATAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR218038 representing NM_173423
Red=Cloning site Green=Tags(s)

MDLKTAVFNAARDGKLRLLTKLLASKSKAEVSSLISEKTNGATPLLMAARYGHLDMVEFLLEQCSASIEV
GGSVNFDGETIEGAPPLWAASAAGHLKVVQSLLNHGASVNNTTLTNSTPLRAACFDGHLEIVKYLVEHKA
DLEVSNRHGHTCLMISCYKGHKEIAQYLLEKGADVNRKSVKGNTALHDCAESGSLDIMKMLLMYCAKMEK
DGYGMTPLLSASVTGHTNIVDFLTHHAQTSKTERINALELLGATFVDKKRDLLGALKYWKKAMNMRYSDR
TNIISKPVPQTLIMAYDYAKEVNSAEELEGLIADPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYAD
SGNFKRCINLWKYALDMQQSNLDPLSPMTASSLLSFAELFSFMLQDRAKGLLGTTVTFDDLMGILCKSVL
EIERAIKQTQCPADPLQLNKALSIILHLICLLEKVPCTVEQDHFKKQTIYRFLKLHPRGKNNFSPLHLAV
DKNTTCVGRYPVCKFPSLQVTAILIECGADVNVRDSDDNSPLHIAALNNHPDIMNLLIKSGAHFDATNLH
KQTASDLLDEKEIAKNLIQPINHTTLQCLAARVIVNHRIYYKGNIPEKLETFVSLHR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_173423
ORF Size 1851 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_173423.4, NP_775599.1
RefSeq Size 2866 bp
RefSeq ORF 1854 bp
Locus ID 240263
UniProt ID Q8CEF1
MW 68.6 kDa
Gene Summary Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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