Bcorl1 (NM_178782) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	6720425J07Rik; B930011H20Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR217927 representing NM_178782 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCTCTACAGCACCGCTCTACAGCGGCGTGCACAACTGGACCAGTTCTGACCGGATTCGCATGTGTG GCATCAACGAGGAGAGAAGAGCACCTCTTTCTGATGAGGAGTCCACGACAGGTGGCTGCCAGCACTTTGG ATCTCAGGAGTTTTGTGTCAGCAGCAGTTTTTCCAAGGTGGAGCTCACGGCAGTTGGAAGTGGCAGCAAT GCCCGGGGGACAAACCCAGATGGCAATACCACAGAAAAACTTGGGCACAGGTCAGAAGATCAGTCTGATG ACCCTCAGCCAAAAATGGACTATGTTGGGAATCCAGCAGAGGCTGAGGGCCTCCTGGTGCCACTGAGCAG CCCGGGAGACGGGCTCAAGCTGCCCACTCCTGACAGCACTGAGGCCAGCCACAGCAGAGCCAACTGCTCC TGGACTCCCTTAAGCACCCAAATGAGCAAACAGGTTGACTGCTCGCCAGCCGGGGTAAAGGCTTTGGACT CTCGGCACGGTGTTGGGGAGAAGAACACTTTCATTTTGGCAACTCTGGGAACTGGAGTCCCTGTGGAGGG TACCCTGCCTCTAGTTACCACTAACTTCAGTCAGCTGCCGGCACCTATCTGCCCTCCTGCCCCTGGATCG GCCTCTGGGACCCCCTCGGTTCCCGATCCATTCCAAGTTCCCCTTTCTGTCCCAGCTCCAGTGCCCCACT CAGGACTAGTTCCAGTCCAAGTTGCCACATCGGCTTCAGCTCCTTCCCCTCCCCTAGCCCCAGCAGCGCC CTCGGTGCCTACTCTCATCTCTGATTCAAACCCTCTCTCTGTGTCAGCCTCTGTCTTGGTGCCAGTACCA GTGTCTGCTCCCCACTCGGTCCCTGTGCCCTTGTCGGCTCCTGCTCCGACCCCTCTCACAGTCTCTGTGT CCGCACCTCCCTTGGCTCTGATCCAGGCTCCCGTGCCTCCCTCGGCTCCCACCTTGGTCCTTGCTTCTGT CCCAACTCCAGTTCTAGCTCCCATGCCAGCATCCACACCTCCAGCAGCTCCTGCCCCCCCATCTGTACCA ATGCCCACTCCGACCCCTTCCTCTGGCCCACCCTCTACCCCCACCCTTATTCCTGCCTTTGCTCCTACAC CGGTGCCTGCGCCCACCCCAGCCCCAATCTTTACTCCAGCCCCCACGCCCATGCCGGCTGCCACACCAGC TGCTATCCCCACCTCGGCGCCCATTCCAGCCTCCTTCAGTTTGAGTCGAGTGTGTTTTCCTGCAGCTCAG GCACCAGCTATGCAAAAAGTCCCCCTGTCTTTTCAACCAGGGACAGTGCTGACCCCTAACCAGCCTCTGG TATACATCCCACCTCCGAGTTGTGGCCAGCCCCTCAGTGTGGCTACACTGCCAACCACTCTGGGTGTGTC TTCCACACTCACACTCCCGGTCCTGCCATCTTACCTGCAGGACAGGTGTCTCCCAGGTGTGTTGGCCTCT CCAGACCTCCGCTCTTATCCATGTGCATTTTCTGTGGCCCGACCTTTGGCTTCAGATTCAAAGTTGGTCT CTTTAGAGGTGAACAGGCTCTCCTGTACTTCCCCATCCAGCAGCACTAACAGCCAGCCTGCTCCCGATGG GGTCCCTGGACCTTTGGCAGATACCTCCCTCACCACTGCTTCTGCCAAGGTGCTTCCAACTTCACAGCTT TTGCTGCCAGCCCCCAGTGGGAGCTCAGTCCCACCACACCCTTCCAAGATGCCAGGTGGCACCGATCAAC AAACAGAAGGGACTTCTGTCACCTTCTCTCCTCTCAAGTCACCTCCACAGCTGGAGAGAGAGATGGCCTC TCCACCCGAGTGCAGTGAGATGCCTCTTGACCTCTCTGCCAAGTCCAACCGCCAGAAGCTTCCATTGCCG AACCAGCGTAAAACACCTCCCATGCCTGTGTTGACCCCTGTGCACACCAGCAGCAAGGCCCTCCTTTCCA CAGTCCTGTCTAGGTCTCAACGCACAACCCAGGCTGCCGGCAGCAACGTTACTTCCTGCCTAGGCTCCAC CTCTTCACCTTTTGTTATCTTTCCTGAGATGGTGAGGAATGGAGACCCAAGCACTTGGGTAAAAAACTCA ACTGCTCTGATCAGCACCATTCCTGGCACATATGTGGGAGTGGCCAACCCAGTGCCTGCATCCCTGCTGT TGAACAAGGACCCTAACCTGGGCCTTAACCGAGATCCTCGTCATCTCCCCAAGCAGGAGCCCATCTCCAT CATTGATCAAGGAGAGCCTAAGAGCACTAGTGCCACTTGTGGCAAGAAAGGCAGCCAGGCGGGTGCTGAA GGACAGCCAAGCACAGTGAAATCCCGTTATACCCCAGCCCGCATTGCCCCTGGGCTGCCAGGGTGCCAAA CCAAGGAACTGTCTTTATGGAAGCCTACAGGCCTGACAAATATGTACCCACGGTGTTCCATCAATGGAAA GCCCACTAGCACCCAGGTTCTACCTGTTGGCTGGTCACCATACCACCAAGCATCTCTGCTTTCTATTGGC ATTTCTAGTGCCGGGCAACTAACCCCCAGTCAGGGGGTGCCTATCAGGCCCACCAGCATTGTTTCTGAGT TTTCTGGTGTGTCACCTCTTGGTTCCAGTGAAACTGTTCATGGACTTCCTGAGGGGCAGCCACGGCCTGG GGGCCCTTTTGCTCCAGAGCAGGATGCTGTCACAAAGAACAAAAATTGCCGAATTGCAGCCAAGCCTTAT GAAGAACAAGTTAATCCTGTCCTCTTGACTCTCAGCCCTCAGTCAGGGACCCTGGCACTGTCTGTTCAGC CTAGCAGTGGGGACATGGGAGTAAATCAGGGATCCGAGGAATCTGAGAGCCACCTCTGCTCTGACAGTAC CCCTAAGATGGAAGGCCCCCAGGCAGCCTGTGGCCTGAAGCTGGCAGGAGACACAAAGCCTAAAAACCAA GTGCTGGCTACCTATATGTCCCACGAGCTGGTCCTGGCCAACCCACAGAACTTGTGCAAGATGCCTGAGC TGCCTTTGCTACCTCATGACAGCCACTCTAAGGAACTCATTTTAGACGTGGTCCCCAGCAGCGAGAGGGG CCCCAGCACAGACCTTTCTCAGCTTGGAAGTCAGGTGGATCTGGGGCGGGTGAAAATGGAGAAAGCCGAT GGCGATGTGGTCTTCAATTTAGCCAACTGCTTCCGAGCTGATGGTCTCCCAGCAGTTCCTCAGAGAGGCC AGGCTGAAGCTCGGGCTAACGCTGGGCAAGCTCGAGTGAAACGGGAAAGCATTGGGGTCTTTACTTGCAA GAACAGTTGGCAGCCAGACGAGGAGACAGAATCTCTGCCACCCAAGAAGGTCAAGTGTAACAAAGAAAAG GAAATTGAGGAAGAACCACGGCAGCAGCCACCACCACAGCCACACGATAAGCCCATGGTCCGGAGTTCCT TGGGGTCCAAGTGCCGGAAGCTGCCTGGAGACCCCCAGGAACCCACCAAGAAAAGCCCCAGAGGGGCTTT AGATTCAGGAAAAGAGCACAATGGAGTCAGGGGAAAGCACAAGCACCGGAAGCCAACAAAGCCCGAGTCC CAGCCTCCAGGAAAACGAACAGATGGTCATGAGGAAGGTTCCTTGGAAAAGAAAGCAAAGAACAGTTTCC GTGACTTCATCCCTGTGGTTCTGAGCACTCGGACACGCAGTCAGTCTGGTAGCATCTGTAGCTCTTTTGC TGGTATGGCAGACAGTGACATGGGAAGCCAGGAGGTTTTCCCTACAGAGGAAGAGGAGGAGGTCGCTCCC ACCCCAGCAAAGAGGAGAAAAGTCAGAAAGACCCAAAGGGATACCCAGTACCGCAGCCATCATGCCCAGG ACAAAACCCTGCTGAGCCAAGGCCGCAGGCATCTGTGGCGAGCCCGAGAGATGCCCTGGAGGACAGAGGC TGCCCGGCAAATGTGGGACACCAATGAAGAGGAGGAAGATGATGAGGAAGAGGGCCTCGTGAAGAGAAAG AAACGGAGACGGCAGAAGAGCCGAAAATACCAGACCGGGGAGTACCTGATTGAACAAGAAGAGCAGAGGC GGAAAGGGAGAGCAGATTCAAAGGCCCGTAAGCAGAAGACTTCCTCCCAAAGTTCAGAGCACTGCCTCAG GAACAGGAACCTTCTCTTGTCCAGCAAAGCCCAGGGGATCTCGGACTCACCAAACGGTTTCCTTCCAGAT AACCTGGAGGAGCCAGCCTGCCTTGAAAACCCAGAAAAGCCGTCAGGAAAGCGAAAGTGCAAGACCAAGC ACATGGCAAACGCCTCAGAAGAGGCAAGGAGCAAAGGTCGTTGGAGCCAGCAGAAGACACGATCTTCTAA ATCTCCCACTCCTGTGAAGCCCACAGAACCATGTACACCCTCGAAGTACCGAAGTGCTGGCCCTGAAGAG GCCTCAGAGTCACCCACTGCCCGACAGATTCCCCCAGAGGCACGGAGACTTATAGTGAATAAAAATGCCG GGGAGACCCTGCTGCAGAGAGCAGCTCGCCTGGGTTATAAGGACGTAGTTCTCTACTGCCTCCAAAAGCA CAGCGAGGATGTGAATCACCGTGACAACGCGGGCTACACAGCCTTGCACGAAGCCTGTTCCCGGGGCTGG ACTGATATCCTGAACATCCTCTTGCAACATGGGGCTAATGTGAACTGCAGCTCGCAGGATGGCACAAGGC CAGTTCATGATGCTGTGGTCAATGACAACCTAGAAACCATCTGGCTCTTGCTATCCTACGGAGCTGATCC CACACTGGCTACCTACTCGGGTCAGACAGCCATGAAGCTGGCCAGCAGTGATAACATGAAGCGCTTTCTC AGTGATCACCTCTCGGATCTTCAGGGCAGGGCAGAGGGCGATCCCCGTGCGTCCTGGGATTTCTACAGCA GTTCTGTGTTGGAGAAAAAAGACGGGTTTGCCTGTGACCTCCTCCATAATCCTCCTGGGAGTGCCGAGCA AGGAGATGATTCAGAACAGGATGACTTCATGTTCGAACTCTCAGACAAGCCTCTCCTCCCTTGTTACAAC CTCCAAGTGTCAGTGTCCCGTGGACCCTGCAACTGGTTCCTCTTCAGCGATGTCCTGAAGAGGCTGAAGC TCTCCTCAAGAATCTTTCAGGCCCGCTTCCCACACCTTGAAATCACCACCCTGCCCAAGGCTGAGTTCTA CCGGCAGGTGGCCTCCAGTCAGCTGCTGAGCCCTGCTGAGAGGCCTGGGAGCTTGGAGGACAGATCTCCC CCAGGGTCTTCTGAGACTGTGGAGCTGGTGCAGTATGAGCCAGAGCTGCTGCGGCTCCTAGGGTCTGAGG TGGAATACCAGTCTTGGAGCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR217927 representing NM_178782 Red=Cloning site Green=Tags(s) MISTAPLYSGVHNWTSSDRIRMCGINEERRAPLSDEESTTGGCQHFGSQEFCVSSSFSKVELTAVGSGSN ARGTNPDGNTTEKLGHRSEDQSDDPQPKMDYVGNPAEAEGLLVPLSSPGDGLKLPTPDSTEASHSRANCS WTPLSTQMSKQVDCSPAGVKALDSRHGVGEKNTFILATLGTGVPVEGTLPLVTTNFSQLPAPICPPAPGS ASGTPSVPDPFQVPLSVPAPVPHSGLVPVQVATSASAPSPPLAPAAPSVPTLISDSNPLSVSASVLVPVP VSAPHSVPVPLSAPAPTPLTVSVSAPPLALIQAPVPPSAPTLVLASVPTPVLAPMPASTPPAAPAPPSVP MPTPTPSSGPPSTPTLIPAFAPTPVPAPTPAPIFTPAPTPMPAATPAAIPTSAPIPASFSLSRVCFPAAQ APAMQKVPLSFQPGTVLTPNQPLVYIPPPSCGQPLSVATLPTTLGVSSTLTLPVLPSYLQDRCLPGVLAS PDLRSYPCAFSVARPLASDSKLVSLEVNRLSCTSPSSSTNSQPAPDGVPGPLADTSLTTASAKVLPTSQL LLPAPSGSSVPPHPSKMPGGTDQQTEGTSVTFSPLKSPPQLEREMASPPECSEMPLDLSAKSNRQKLPLP NQRKTPPMPVLTPVHTSSKALLSTVLSRSQRTTQAAGSNVTSCLGSTSSPFVIFPEMVRNGDPSTWVKNS TALISTIPGTYVGVANPVPASLLLNKDPNLGLNRDPRHLPKQEPISIIDQGEPKSTSATCGKKGSQAGAE GQPSTVKSRYTPARIAPGLPGCQTKELSLWKPTGLTNMYPRCSINGKPTSTQVLPVGWSPYHQASLLSIG ISSAGQLTPSQGVPIRPTSIVSEFSGVSPLGSSETVHGLPEGQPRPGGPFAPEQDAVTKNKNCRIAAKPY EEQVNPVLLTLSPQSGTLALSVQPSSGDMGVNQGSEESESHLCSDSTPKMEGPQAACGLKLAGDTKPKNQ VLATYMSHELVLANPQNLCKMPELPLLPHDSHSKELILDVVPSSERGPSTDLSQLGSQVDLGRVKMEKAD GDVVFNLANCFRADGLPAVPQRGQAEARANAGQARVKRESIGVFTCKNSWQPDEETESLPPKKVKCNKEK EIEEEPRQQPPPQPHDKPMVRSSLGSKCRKLPGDPQEPTKKSPRGALDSGKEHNGVRGKHKHRKPTKPES QPPGKRTDGHEEGSLEKKAKNSFRDFIPVVLSTRTRSQSGSICSSFAGMADSDMGSQEVFPTEEEEEVAP TPAKRRKVRKTQRDTQYRSHHAQDKTLLSQGRRHLWRAREMPWRTEAARQMWDTNEEEEDDEEEGLVKRK KRRRQKSRKYQTGEYLIEQEEQRRKGRADSKARKQKTSSQSSEHCLRNRNLLLSSKAQGISDSPNGFLPD NLEEPACLENPEKPSGKRKCKTKHMANASEEARSKGRWSQQKTRSSKSPTPVKPTEPCTPSKYRSAGPEE ASESPTARQIPPEARRLIVNKNAGETLLQRAARLGYKDVVLYCLQKHSEDVNHRDNAGYTALHEACSRGW TDILNILLQHGANVNCSSQDGTRPVHDAVVNDNLETIWLLLSYGADPTLATYSGQTAMKLASSDNMKRFL SDHLSDLQGRAEGDPRASWDFYSSSVLEKKDGFACDLLHNPPGSAEQGDDSEQDDFMFELSDKPLLPCYN LQVSVSRGPCNWFLFSDVLKRLKLSSRIFQARFPHLEITTLPKAEFYRQVASSQLLSPAERPGSLEDRSP PGSSETVELVQYEPELLRLLGSEVEYQSWSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_178782
ORF Size	5343 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_178782.4, NP_848897.3
RefSeq Size	6142 bp
RefSeq ORF	5346 bp
Locus ID	320376
UniProt ID	A2AQH4
MW	190.5 kDa
Gene Summary	Transcriptional corepressor. May specifically inhibit gene expression when recruited to promoter regions by sequence specific DNA-binding proteins such as BCL6. This repression may be mediated at least in part by histone deacetylase activities which can associate with this corepressor (By similarity).[UniProtKB/Swiss-Prot Function]

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