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Gpr25 (NM_001101516) Mouse Tagged ORF Clone
CAT＃: MR217813
- TrueORF®
Gpr25 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 25 (Gpr25)
ORF Plasmid: tGFP
"NM_001101516" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gm1300 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217813 representing NM_001101516
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTCCACCGAACCCTGGAGTCCTAGCTGGGGCACGTTGTCCTGGGACTATTCGGGCTCGGGCTCCC TGGACCAAGTGGAGTTGTGCCCTGCTTGGAACCTGCCTTACGGCCACGCCATCATCCCAGCGCTCTACCT GGCGGCCTTCGCCGTGGGCCTGCCGGGCAACGCCTTCGTGGTGTGGCTACTGAGCCGGCAGCGCGGGCCG CGGCGGCTGGTGGACACCTTCGTGCTGCACTTGGCGGCCGCCGACCTGGGCTTCGTGCTCACGCTGCCGC TGTGGGCCGCGGCAGAGGCGCGCGGCGGCCTCTGGCCCTTCGGCGACGGCCTGTGCAAAGTCAGCAGCTT CGCGCTGGCGGTCACGCGCTGCGCGGGCGCGCTGCTGCTGGCGGGCATGAGCGTGGACCGCTACCTGGCG GTGGGCCGGCCGCTGAGCGCGCGCCCGCTGCGGAGCGCGCGCTGTGTGCGAGCCGTGTGCGGCGCCGCCT GGGCTGCGGCGTTCCTGGCGGGTTTGCCGGCCTTGCTCTACCGGGGACTGCAGCCGAGCCTGGACGGCGT GGGCAGCCAGTGCGCCGAAGAGCCCTGGGAAGCGCTCCAGGGTGTCGGGCTGCTGCTGCTGCTACTGACC TTCGCGCTGCCGCTGGCTGTCACCCTGATCTGCTACTGGCGCGTGTCGCGCCGCCTGCCGCGCGTCGGTA GGGCCCGGAGCAACTCCCTGCGCATCATCTTCACCGTGGAGAGCGTCTTCGTGGGCTGCTGGCTGCCCTT CGGCGTCCTGCGCTCCCTCTTCCACCTGGCGCGGCTCCAGGCGCTGCCGCTGCCCTGCTCCCTGCTGCTG GCTCTGCGCTGGGGTCTGACTGTCACCACCTGCCTGGCTTTCGTCAATAGCAGTGCGAACCCGGTCATCT ACCTCCTGTTGGACCGCTCATTCCGCGCCCGGGCCCGGTTCGGGCTGTGCGCGCGCGCCGGGCGCCAGGT GCGCAGGATCAGCTCGGCCTCCTCGCTCTCCAGGGACGACAGTTCGGTGTTCCGGGGCCGGTCCCCGAAG GTGAACTCTGCCTCGGCCACTTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217813 representing NM_001101516
Red=Cloning site Green=Tags(s) MQSTEPWSPSWGTLSWDYSGSGSLDQVELCPAWNLPYGHAIIPALYLAAFAVGLPGNAFVVWLLSRQRGP RRLVDTFVLHLAAADLGFVLTLPLWAAAEARGGLWPFGDGLCKVSSFALAVTRCAGALLLAGMSVDRYLA VGRPLSARPLRSARCVRAVCGAAWAAAFLAGLPALLYRGLQPSLDGVGSQCAEEPWEALQGVGLLLLLLT FALPLAVTLICYWRVSRRLPRVGRARSNSLRIIFTVESVFVGCWLPFGVLRSLFHLARLQALPLPCSLLL ALRWGLTVTTCLAFVNSSANPVIYLLLDRSFRARARFGLCARAGRQVRRISSASSLSRDDSSVFRGRSPK VNSASATW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001101516 |
| ORF Size | 1074 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001101516.2 |
| RefSeq Size | 1960 bp |
| RefSeq ORF | 1077 bp |
| Locus ID | 383563 |
| UniProt ID | P0C5I1 |
| MW | 39.2 kDa |
| Gene Summary | Orphan receptor.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC214780 | Gpr25 (untagged) - Mouse G protein-coupled receptor 25 (Gpr25), (10ug) |
CNY 3,990.00 |
|
| MG217813 | Gpr25 (tGFP-tagged) - Mouse G protein-coupled receptor 25 (Gpr25), (10ug) |
CNY 3,710.00 |
|
| MR217813L3 | Lenti ORF clone of Gpr25 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 25 (Gpr25) |
CNY 5,230.00 |
|
| MR217813L4 | Lenti ORF clone of Gpr25 (mGFP-tagged) - Mouse G protein-coupled receptor 25 (Gpr25) |
CNY 5,230.00 |
