Man2a1 (NM_008549) Mouse Tagged ORF Clone
CAT#: MR217804
- TrueORF®
Man2a1 (Myc-DDK-tagged) - Mouse mannosidase 2, alpha 1 (Man2a1)
ORF Plasmid: tGFP
"NM_008549" in other vectors (4)
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CNY 8,008.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Mana-2; Mana2; Map-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR217804 representing NM_008549
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTAAGTCGCCAGTTCACCGTGTTTGGCAGCGCGATCTTCTGCGTCGTAATCTTCTCACTCTACC TGATGCTGGACAGGGGTCACTTGGACTACCCTCGGGGCCCGCGCCAGGAGGGCTCCTTTCCGCAGGGCCA GCTTTCAATATTGCAAGAAAAGATTGACCATTTGGAGCGTTTGCTCGCTGAGAACAACGAGATCATCTCA AATATCAGAGACTCAGTCATCAACCTGAGCGAGTCTGTGGAGGACGGCCCGCGGGGGTCACCAGGCAACG CCAGCCAAGGCTCCATCCACCTCCACTCGCCACAGTTGGCCCTGCAGGCTGACCCCAGAGACTGTTTGTT TGCTTCACAGAGTGGGAGTCAGCCCCGGGATGTGCAGATGTTGGATGTTTACGATCTGATTCCTTTTGAT AATCCAGATGGTGGAGTTTGGAAGCAAGGATTTGACATTAAGTATGAAGCGGATGAGTGGGACCATGAGC CCCTGCAAGTGTTTGTGGTGCCTCACTCCCATAATGACCCAGGTTGGTTGAAGACTTTCAATGACTACTT TAGAGACAAGACTCAGTATATTTTTAATAACATGGTCCTAAAGCTGAAAGAAGACTCAAGCAGGAAGTTT ATGTGGTCTGAGATCTCTTACCTTGCAAAATGGTGGGATATTATAGATATTCCGAAGAAGGAAGCTGTTA AAAGTTTACTACAGAATGGTCAGCTGGAAATTGTGACCGGTGGCTGGGTTATGCCTGATGAAGCCACTCC ACATTATTTTGCCTTAATTGACCAACTAATTGAAGGGCACCAATGGCTGGAAAAAAATCTAGGAGTGAAA CCTCGATCGGGCTGGGCCATAGATCCCTTTGGTCATTCACCCACAATGGCTTATCTTCTAAAGCGTGCTG GATTTTCACACATGCTCATCCAGAGAGTCCATTATGCAATCAAAAAACACTTCTCTTTGCATAAAACGCT GGAGTTTTTCTGGAGACAGAATTGGGATCTTGGATCTGCTACAGACATTTTGTGCCATATGATGCCCTTC TACAGCTACGACATCCCTCACACCTGTGGGCCTGATCCTAAAATATGCTGCCAGTTTGATTTTAAACGGC TTCCTGGAGGCAGATATGGTTGTCCCTGGGGAGTTCCCCCAGAAGCAATATCTCCTGGAAATGTCCAAAG CAGGGCTCAGATGCTATTGGATCAGTACCGGAAAAAGTCAAAACTTTTCCGCACTAAAGTTCTGCTGGCT CCACTGGGAGACGACTTTCGGTTCAGTGAATACACAGAGTGGGATCTGCAGTGCAGGAACTACGAGCAAC TGTTCAGTTACATGAACTCGCAGCCTCATCTGAAAGTGAAGATCCAGTTTGGAACCTTGTCAGATTATTT CGACGCATTGGAGAAAGCGGTGGCAGCCGAGAAGAAGAGTAGCCAGTCTGTGTTCCCTGCCCTGAGTGGA GACTTCTTCACGTACGCTGACAGAGACGACCATTACTGGAGTGGCTACTTCACGTCCAGACCTTTCTACA AACGAATGGACAGAATAATGGAATCTCGTATAAGGGCTGCTGAAATTCTTTACCAGTTGGCCTTGAAACA AGCTCAGAAATACAAGATAAATAAATTTCTTTCATCACCTCATTACACAACACTGACAGAAGCCAGAAGG AACTTAGGACTATTTCAGCATCATGATGCCATCACAGGAACCGCGAAAGACTGGGTGGTTGTGGACTATG GTACCAGACTCTTTCAGTCATTAAATTCTTTGGAGAAGATAATTGGAGATTCTGCATTTCTTCTCATTTT AAAGGACAAAAAGCTGTACCAGTCAGATCCTTCCAAAGCCTTCTTAGAGATGGATACGAAGCAAAGTTCA CAAGATTCTCTGCCCCAAAAAATTATAATACAACTGAGCGCACAGGAGCCAAGGTACCTTGTGGTCTACA ATCCCTTTGAACAAGAACGGCATTCAGTGGTGTCCATCCGGGTAAACTCCGCCACAGTGAAAGTGCTGTC TGATTCGGGAAAACCGGTGGAGGTTCAAGTCAGTGCAGTTTGGAACGACATGAGGACAATTTCACAAGCA GCCTATGAGGTTTCTTTTCTAGCTCATATACCACCACTGGGACTGAAAGTGTTTAAGATCTTAGAGTCAC AAAGTTCAAGCTCACACTTGGCTGATTATGTCCTATATAATAATGATGGACTAGCAGAAAATGGAATATT CCACGTGAAGAACATGGTGGATGCTGGAGATGCCATAACAATAGAGAATCCCTTCCTGGCGATTTGGTTT GACCGATCTGGGCTGATGGAGAAAGTGAGAAGGAAAGAAGACAGTAGACAGCATGAACTGAAGGTCCAGT TCCTGTGGTACGGAACCACCAACAAAAGGGACAAGAGCGGTGCCTACCTCTTCCTGCCTGACGGGCAGGG CCAGCCATATGTTTCCCTAAGACCGCCCTTTGTCAGAGTGACACGTGGAAGGATCTACTCAGATGTGACC TGTTTCCTCGAACACGTTACTCACAAAGTCCGCCTGTACAACATTCAGGGAATAGAAGGTCAGTCCATGG AAGTTTCTAATATTGTAAACATCAGGAATGTGCATAACCGTGAGATTGTAATGAGAATTTCATCTAAAAT AAACAACCAAAATAGATATTATACTGACCTAAATGGATATCAGATTCAGCCTAGAAGGACCATGAGCAAA TTGCCTCTTCAAGCCAACGTTTACCCGATGTGCACAATGGCGTATATCCAGGATGCTGAGCACCGGCTCA CGCTGCTCTCTGCTCAGTCTCTAGGTGCTTCCAGCATGGCTTCTGGTCAGATTGAAGTCTTCATGGATCG AAGGCTCATGCAGGATGATAACCGTGGCCTTGGGCAAGGCGTCCATGACAATAAGATTACAGCTAATTTG TTTCGAATCCTCCTCGAGAAGAGAAGCGCTGTGAACATGGAAGAAGAAAAGAAGAGCCCTGTCAGCTACC CTTCCCTCCTCAGCCACATGACTTCGTCCTTCCTCAACCATCCCTTTCTCCCCATGGTACTAAGTGGCCA GCTCCCCTCCCCTGCCTTTGAGCTGCTGAGTGAATTTCCTCTGCTGCAGTCCTCTCTACCTTGTGATATC CATCTGGTCAACCTGCGGACAATACAATCAAAGATGGGCAAAGGCTATTCGGATGAGGCAGCCTTGATCC TCCACAGGAAAGGGTTTGATTGCCAGTTCTCCAGCAGAGGCATCGGGCTACCCTGTTCCACTACTCAGGG AAAGATGTCAGTTCTGAAACTTTTCAACAAGTTTGCTGTGGAGAGTCTCGTCCCTTCCTCTCTGTCCTTG ATGCACTCCCCTCCAGATGCCCAGAACATGAGTGAAGTCAGCCTGAGCCCCATGGAGATCAGCACGTTCC GTATCCGCTTGCGTTGGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217804 representing NM_008549
Red=Cloning site Green=Tags(s) MKLSRQFTVFGSAIFCVVIFSLYLMLDRGHLDYPRGPRQEGSFPQGQLSILQEKIDHLERLLAENNEIIS NIRDSVINLSESVEDGPRGSPGNASQGSIHLHSPQLALQADPRDCLFASQSGSQPRDVQMLDVYDLIPFD NPDGGVWKQGFDIKYEADEWDHEPLQVFVVPHSHNDPGWLKTFNDYFRDKTQYIFNNMVLKLKEDSSRKF MWSEISYLAKWWDIIDIPKKEAVKSLLQNGQLEIVTGGWVMPDEATPHYFALIDQLIEGHQWLEKNLGVK PRSGWAIDPFGHSPTMAYLLKRAGFSHMLIQRVHYAIKKHFSLHKTLEFFWRQNWDLGSATDILCHMMPF YSYDIPHTCGPDPKICCQFDFKRLPGGRYGCPWGVPPEAISPGNVQSRAQMLLDQYRKKSKLFRTKVLLA PLGDDFRFSEYTEWDLQCRNYEQLFSYMNSQPHLKVKIQFGTLSDYFDALEKAVAAEKKSSQSVFPALSG DFFTYADRDDHYWSGYFTSRPFYKRMDRIMESRIRAAEILYQLALKQAQKYKINKFLSSPHYTTLTEARR NLGLFQHHDAITGTAKDWVVVDYGTRLFQSLNSLEKIIGDSAFLLILKDKKLYQSDPSKAFLEMDTKQSS QDSLPQKIIIQLSAQEPRYLVVYNPFEQERHSVVSIRVNSATVKVLSDSGKPVEVQVSAVWNDMRTISQA AYEVSFLAHIPPLGLKVFKILESQSSSSHLADYVLYNNDGLAENGIFHVKNMVDAGDAITIENPFLAIWF DRSGLMEKVRRKEDSRQHELKVQFLWYGTTNKRDKSGAYLFLPDGQGQPYVSLRPPFVRVTRGRIYSDVT CFLEHVTHKVRLYNIQGIEGQSMEVSNIVNIRNVHNREIVMRISSKINNQNRYYTDLNGYQIQPRRTMSK LPLQANVYPMCTMAYIQDAEHRLTLLSAQSLGASSMASGQIEVFMDRRLMQDDNRGLGQGVHDNKITANL FRILLEKRSAVNMEEEKKSPVSYPSLLSHMTSSFLNHPFLPMVLSGQLPSPAFELLSEFPLLQSSLPCDI HLVNLRTIQSKMGKGYSDEAALILHRKGFDCQFSSRGIGLPCSTTQGKMSVLKLFNKFAVESLVPSSLSL MHSPPDAQNMSEVSLSPMEISTFRIRLRWT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008549 |
ORF Size | 3450 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008549.2, NP_032575.2 |
RefSeq Size | 6091 bp |
RefSeq ORF | 3453 bp |
Locus ID | 17158 |
UniProt ID | P27046 |
MW | 132.1 kDa |
Gene Summary | Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223734 | Man2a1 (untagged) - Mouse mannosidase 2, alpha 1 (Man2a1), (10ug) |
CNY 14,820.00 |
|
MG217804 | Man2a1 (tGFP-tagged) - Mouse mannosidase 2 alpha 1 (Man2a1), (10ug) |
CNY 11,210.00 |
|
MR217804L3 | Lenti ORF clone of Man2a1 (Myc-DDK-tagged) - Mouse mannosidase 2, alpha 1 (Man2a1) |
CNY 12,070.00 |
|
MR217804L4 | Lenti ORF clone of Man2a1 (mGFP-tagged) - Mouse mannosidase 2, alpha 1 (Man2a1) |
CNY 12,070.00 |