Rab11fip3 (NM_001162868) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Cart1; D030060O14Rik; mKIAA0665; Rab11-FIP3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR217631 representing NM_001162868 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGTGCCAGCCGACCTCCCTGTCAGACCACGACCAGCCAGCGTCTGGGCCCCAGCGTGGGGTCA TGGGGCTGGTGGGGCCCGACGCTCCTCGCGGCTGGTCAGAAGAGCCCGAGGAACACGCCCAGCTTCAACG GTGGCCAGAGGGACCCAACGCGCCAATCTGCTGGCCCGAGGAGGTAGAGGAGCCTCACGCTCCTAGTCGC TGGGCCAAAGAGCCCAATGCCCCTCGCTGCTCGTCCCAGGAGCCCGATGAATCCTGTCACTTGGCAGAGG AGCTGGAGGAATCCGACTCCCCTCGCTGCTGGCCCCAGGAGCCAGACACACCGTGCCACTTGGCCAAGGA GTTGGAGGAACCCGACGCCCCTCGCTGCCTGCCCCAGGAGCCCGACACACCGTGCTACTTGGCCAAAGAG CTAGAGGAACCCAACATCCCTCGGTGCTGGCCCCAGGAGCCCGACGTGCCGTGCCACTTGGCCAAGGAGC TAGAGGAACCCGACGCCCCTCGCTGCTGGCCCCAGGAACCAGACGCATTCTGTCACTTGCTCAAGGAGGT GGAGGAACCTGATGCCCTTCGCTGCTGGCTTCAAGGGCCAGACGCACCGTGCCACTTGGCCAAGGAACTG GAGGACCTCGACTCCCCTCGCTGCTGGCCCCAGGAGCCCGACGAATCCTGTCACTTGGCTAAGGAGCTGG AGGAACCAGACGCACCGTGCCACTTAGCCAAAGAGCTGGAGGAACCCGACGCCCCTCGCTGCTGGCCCCA GGAGCCCGACGTGCCCTGTCTCTTGGCCAAAAAGTGGGAGGAATCCGACGCACCGTGCCTCTTGACGGAG GAGCTGGAAGAGCCTGACGCCCTTCACTGCTGGCCCCAGGAGTCAGAGGCACCGTGTTTGTTGGCCAAGG AGCTAGAAGAGCCTGATGCCTCTCACAGCTGTCCTCAGGAGGCGGACACAGGGTGCCTCTCGGCCAAGGA GCCAGAGGAGCCTGATGTCTCTCACCTGTGGCAGGGGGTACCCGATGCACCGTGCCTCTTGGTCAAGGAA CCAGAGGAGGCCGATGCACTTCACTGTTGTTGGCCCGAGGAATCCGAGGAGCCGGATGCTCTCAACCCAC CGTGTTTCTGGGCCAATGAACCAGACGAGCCCGATCCATCACGATGTTGGTCAGAGGAGCCCCAGGTGCT GTGCCTCTGGCCGGAGGAGCAGAACACAAAGCGGTGCTGGCAGGAAGAGCCCGATGCACCCTGCTTCTGG CCCGAGGATCGAGAGGAACCCATAGTTTCGTGTCTTCAATTTAAAGAGCCAGAGAAGCCCAAAGTCCGGA GCAGTTGGCCTGAGGAGCTGGAAGACTGCTGCCCTACGCGGGGCCTACCCCTTGAGCCCTTGCTCGCCGA CGGCGAGCTGCTGCAGGCATGTCCCGGGCCACCCTCGGACCCAGGACCAGCGTTGTCGCTGCCCAGCGAG CCCGGGACAGCTCAGGAGGAGGGTGCACGCCTCAGGGCGGTATTTGACGCCCTGGACAGAGATGGGGACG GCTTCGTCCGCATCGAGGATTTCATCCAATTCGCCACGGTCTATGGGGCAGAGCAGGTGAAGGACTTAAC TCAGTACCTGGACCCGAGTGGGCTTGGCGTAATCAGCTTTGAAGATTTCTACCAAGGGATTGTAGCCATC AGAAATGGAGATCCTGATGGCCAGTTGTACAGCGTGGAGCCTGTCCAAGATGAAGAGACCCCTGCCTGTG CCGATGAGTTTGACGACTTTGTCACCTATGAGGCCAACGAGGTGACAGACAGTGCATATATGGGGTCTGA GAGCACCTACAGTGAGTGTGAGACCTTCACAGATGAGGACACCAGCACCCTGGTGCACCCCGAGCTGCAG CCTGAAGGGGACGTGGACAGTGCTGGTGGCTCAGGGGTGCCCTCTGAGTGCCTGGACACCATGGAGGAGC CTGACCATGGTGCATTGCTGCTGCTCCCAGGCAGATCCCGCCCCCACAGCCAAGCTGTCGTCATGGTGAT TGGCAGTGAGGAACATTTTGAAGATTATGGTGAGGGCAATGAGGCAGAACTATCCCCGGAGACCCTCTGC GATGGGGACGGCGAGGACCCTGCTTTTCTCACCCCCAGCCCAGCCAAGCGGCTCTCCAGCAGGAAGGTGG CAAGGTATCTGCACCAGTCGGGGACCCTGACTATGGAGGCCCTGGAGGACCCTCCCCCAGAGCCTGTGGA GTGCCCAGAGGAGGACATTGCAGACAAGGTCATCTTCCTAGAGAGACGGGTGTCAGAGCTGGAGAAGGAC AGTGCAGCTGCTGGCGAGCAGCATGGCAGGCTGAGGCAAGAAAACCTCCAGCTGGTGCACAGAGCCAATG CCTTGGAAGAGCAGCTGAAGGAACAGGAGTTCAGAGCCCAAGAGAAGGTCCTAGAAGAAACCAGGAAGCA GAAGGAACTTCTGTGCAAGATGGAGCGTGAGAAGAGCATTGAGATCGAGAACCTGCAGGCCAGGTTGCAG CAGCTGGATGAGGAGAACAGTGAGCTGCGGTCCTGCACACCCTGTCTGAAGGCCAACATCGAGCGCCTTG AGGAGGAGAAGCAGAAGATGCTGGATGAGATTGAGGAGTTGACACAGCGGCTCAGTGAGGAACAGGAGAA TAAGAGGAAAATGGGGGACAGGCTGAGCCATGAGCGGCACCAATTCCAGAGAGACAAGGAAGCAACCCAG GAGCTGATCGAGGACCTCCGCAAGCAGCTAGAACATCTACAGCTCCTCAGACTGGAGGTGGAGCAGCGAC GGGGCCGCAGCAGCAGCCTGGGCCTGCAGGAGTACAACAGCCGTGCACGGGAGAGCGAGCTGGAGCAGGA GGTCCGCAGACTCAAACAGGACAACCGTAACCTGAAGGAGCAAAATGATGAGCTAAATGGGCAGATCATC ACCCTCAGCATCCAGGGTGCCAAGAGCCTCTTCTCCACGTCTTTCTCAGAATCACTGGCTGCAGAAATCA GCTCTGTCTCCCGAGATGAGCTCATGGAAGCAATCCAGAAGCAGGAGGAGATCAATTTCCGCCTGCAGGA CTACATTGACAGGATCATTGTGGCCATCCTGGAGACCAACCCATCCATCCTAGAGGTCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR217631 representing NM_001162868 Red=Cloning site Green=Tags(s) MELCQPTSLSDHDQPASGPQRGVMGLVGPDAPRGWSEEPEEHAQLQRWPEGPNAPICWPEEVEEPHAPSR WAKEPNAPRCSSQEPDESCHLAEELEESDSPRCWPQEPDTPCHLAKELEEPDAPRCLPQEPDTPCYLAKE LEEPNIPRCWPQEPDVPCHLAKELEEPDAPRCWPQEPDAFCHLLKEVEEPDALRCWLQGPDAPCHLAKEL EDLDSPRCWPQEPDESCHLAKELEEPDAPCHLAKELEEPDAPRCWPQEPDVPCLLAKKWEESDAPCLLTE ELEEPDALHCWPQESEAPCLLAKELEEPDASHSCPQEADTGCLSAKEPEEPDVSHLWQGVPDAPCLLVKE PEEADALHCCWPEESEEPDALNPPCFWANEPDEPDPSRCWSEEPQVLCLWPEEQNTKRCWQEEPDAPCFW PEDREEPIVSCLQFKEPEKPKVRSSWPEELEDCCPTRGLPLEPLLADGELLQACPGPPSDPGPALSLPSE PGTAQEEGARLRAVFDALDRDGDGFVRIEDFIQFATVYGAEQVKDLTQYLDPSGLGVISFEDFYQGIVAI RNGDPDGQLYSVEPVQDEETPACADEFDDFVTYEANEVTDSAYMGSESTYSECETFTDEDTSTLVHPELQ PEGDVDSAGGSGVPSECLDTMEEPDHGALLLLPGRSRPHSQAVVMVIGSEEHFEDYGEGNEAELSPETLC DGDGEDPAFLTPSPAKRLSSRKVARYLHQSGTLTMEALEDPPPEPVECPEEDIADKVIFLERRVSELEKD SAAAGEQHGRLRQENLQLVHRANALEEQLKEQEFRAQEKVLEETRKQKELLCKMEREKSIEIENLQARLQ QLDEENSELRSCTPCLKANIERLEEEKQKMLDEIEELTQRLSEEQENKRKMGDRLSHERHQFQRDKEATQ ELIEDLRKQLEHLQLLRLEVEQRRGRSSSLGLQEYNSRARESELEQEVRRLKQDNRNLKEQNDELNGQII TLSIQGAKSLFSTSFSESLAAEISSVSRDELMEAIQKQEEINFRLQDYIDRIIVAILETNPSILEVK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001162868
ORF Size	3141 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001162868.2
RefSeq Size	4880 bp
RefSeq ORF	3144 bp
Locus ID	215445
UniProt ID	Q8CHD8
MW	119 kDa
Gene Summary	Acts as a regulator of endocytic traffic by participating in membrane delivery. Required for the abcission step in cytokinesis, possibly by acting as an 'address tag' delivering recycling endosome membranes to the cleavage furrow during late cytokinesis (By similarity). Also required for the structural integrity of the endosomal recycling compartment during interphase. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (By similarity).[UniProtKB/Swiss-Prot Function]

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