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Pstpip1 (NM_011193) Mouse Tagged ORF Clone
CAT＃: MR217594
- TrueORF®
Pstpip1 (Myc-DDK-tagged) - Mouse proline-serine-threonine phosphatase-interacting protein 1 (Pstpip1)
ORF Plasmid: tGFP
"NM_011193" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD2BP1; def-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217594 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCCCAGCTGCAGTTCCGAGATGCCTTCTGGTGCAGGGACTTCACGGCCCACACAGGGTATGAGG TGCTACTGCAGAGGCTGCTGGACGGCAGGAAGATGTGCAAGGATGTGGAGGAGCTGCTCAGACAGAGGGC CCAGGCGGAGGAGAGGTACGGGAAGGAGCTGGTGCAGATTGCACGCAAGGCTGGTGGCCAGACAGAGATG AATTCCCTGAGGACCTCCTTTGACTCCCTGAAGCAGCAAACAGAGAATGTGGGCAGTGCACACATCCAGC TGGCCCTGGCCCTGCGTGAGGAGCTGCGGAGCCTGGAGGAGTTCCGAGAGAGACAGAAAGAGCAGCGGAA GAAGTATGAGGCCATCATGGACCGTGTCCAGAAGAGCAAGTTGTCGCTCTACAAGAAGACCATGGAGTCC AAGAAGGCATATGACCAGAAGTGCAGGGATGCAGATGATGCTGAGCAGGCCTTCGAGCGTGTGAGTGCCA ATGGCCACCAGAAGCAAGTAGAAAAGAGCCAGAACAAAGCCAAGCAGTGCAAGGAGTCAGCCACAGAGGC AGAAAGAGTGTACAGGCAAAATATCGAACAACTGGAGAGAGCGAGGACCGAGTGGGAGCAGGAGCACCGG ACTACCTGTGAGGCCTTCCAGTTGCAGGAGTTTGACCGGCTCACCATCCTCCGCAACGCCCTGTGGGTGC ACTGTAACCAGCTCTCCATGCAGTGTGTCAAGGATGATGAGCTCTATGAGGAAGTGCGGCTGACCCTTGA GGGCTGTGATGTGGAAGGTGACATCAATGGCTTCATCCAGTCCAAGAGCACTGGCAGAGAGCCCCCAGCT CCGGTGCCTTATCAGAACTACTATGACAGGGAGGTGACCCCACTGATTGGCAGCCCTAGCGTCCAGCCCT CCTGTGGTGTGATAAAGAGGTTCTCTGGGCTGCTACATGGAAGTCCCAAGACCACACCTTCTGCTCCTGC TGCTTCCACAGAGACTCTGACTCCCACCCCTGAGCGGAATGAGTTGGTCTACGCATCCATCGAAGTGCAG GCGACCCAGGGAAACCTTAACTCATCAGCCCAGGACTACCGGGCACTCTACGACTACACCGCACAGAATT CTGATGAGCTGGACATTTCCGCGGGAGACATCCTGGCGGTCATCCTGGAAGGGGAGGATGGCTGGTGGAC TGTGGAGCGGAACGGACAACGTGGCTTTGTCCCTGGGTCGTACTTGGAGAAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217594 protein sequence
Red=Cloning site Green=Tags(s) MMAQLQFRDAFWCRDFTAHTGYEVLLQRLLDGRKMCKDVEELLRQRAQAEERYGKELVQIARKAGGQTEM NSLRTSFDSLKQQTENVGSAHIQLALALREELRSLEEFRERQKEQRKKYEAIMDRVQKSKLSLYKKTMES KKAYDQKCRDADDAEQAFERVSANGHQKQVEKSQNKAKQCKESATEAERVYRQNIEQLERARTEWEQEHR TTCEAFQLQEFDRLTILRNALWVHCNQLSMQCVKDDELYEEVRLTLEGCDVEGDINGFIQSKSTGREPPA PVPYQNYYDREVTPLIGSPSVQPSCGVIKRFSGLLHGSPKTTPSAPAASTETLTPTPERNELVYASIEVQ ATQGNLNSSAQDYRALYDYTAQNSDELDISAGDILAVILEGEDGWWTVERNGQRGFVPGSYLEKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011193 |
| ORF Size | 1248 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011193.1, NM_011193.2, NM_011193.3, NP_035323.2 |
| RefSeq Size | 1853 bp |
| RefSeq ORF | 1248 bp |
| Locus ID | 19200 |
| UniProt ID | P97814 |
| MW | 47.6 kDa |
| Gene Summary | Involved in regulation of the actin cytoskeleton. May regulate WAS actin-bundling activity. Bridges the interaction between ABL1 and PTPN18 leading to ABL1 dephosphorylation. May play a role as a scaffold protein between PTPN12 and WAS and allow PTPN12 to dephosphorylate WAS. Has the potential to physically couple CD2 and CD2AP to WAS. Acts downstream of CD2 and CD2AP to recruit WAS to the T-cell:APC contact site so as to promote the actin polymerization required for synapse induction during T-cell activation. Down-regulates CD2-stimulated adhesion through the coupling of PTPN12 to CD2. Also has a role in innate immunity and the inflammatory response. Recruited to inflammasomes by MEFV. Induces formation of pyroptosomes, large supramolecular structures composed of oligomerized PYCARD dimers which form prior to inflammatory apoptosis. Binding to MEFV allows MEFV to bind to PYCARD and facilitates pyroptosome formation. Regulates endocytosis and cell migration in neutrophils.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209249 | Pstpip1 (untagged) - Mouse proline-serine-threonine phosphatase-interacting protein 1 (Pstpip1), (10ug) |
CNY 3,990.00 |
|
| MG217594 | Pstpip1 (tGFP-tagged) - Mouse proline-serine-threonine phosphatase-interacting protein 1 (Pstpip1), (10ug) |
CNY 3,710.00 |
|
| MR217594L3 | Lenti ORF clone of Pstpip1 (Myc-DDK-tagged) - Mouse proline-serine-threonine phosphatase-interacting protein 1 (Pstpip1) |
CNY 5,230.00 |
|
| MR217594L4 | Lenti ORF clone of Pstpip1 (mGFP-tagged) - Mouse proline-serine-threonine phosphatase-interacting protein 1 (Pstpip1) |
CNY 5,230.00 |
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