Tcerg1 (NM_001039474) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2410022J09Rik; 2900090C16Rik; AI428505; ca150; CA150b; FBP23; FBP28; p144; Taf2s
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR217424 representing NM_001039474, codon optimized. Due to the complexity of NM_001039474, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGCGGGGAGGAGACGGGGGCGAGGGCGAGAGATTCAACCCAGGGGAGCTGCGGATGGCTCAGC AGCAGGCGCTGAGATTCCGCGGACCTGCACCACCTCCAAACGCTGTGATGAGGGGCCCACCACCACTCAT GAGGCCACCCCCCCCCTTTGGCATGATGAGGGGACCCCCGCCGCCACCACGCCCACCTTTTGGCCGCCCT CCGTTCGATCCAAACATGCCCCCAATGCCACCTCCCGGCGGGATTCCTCCTCCAATGGGGCCCCCGCATC TCCAGCGACCCCCTTTTATGCCTCCACCCATGGGGGCCATGCCCCCTCCGCCCGGCATGATGTTCCCACC AGGCATGCCTCCCGGGACAGCACCAGGCGCACCTGCTCTGCCGCCGACAGAAGAGATCTGGGTGGAGAAT AAGACACCAGACGGAAAAGTGTACTACTATAACGCTCGAACTCGGGAGAGTGCCTGGACTAAGCCCGATG GAGTGAAGGTTATCCAACAATCCGAGCTCACGCCCATGCTGGCAGCTCAGGCCCAGGTTCAGGCCCAGGC GCAGGCCCAGGCTCAGGCCCAGGCACAAGCCCAGGCACAGGCCCAGGCCCAGGCACAGGCTCAAGCTCAG GCTCAAGCGCAGGCACAGGCTCAAGCCCAGGCCCAGGCCCAGGCGCAGGCCCAGGCCCAGGCCCAGGCTC AGGCCCAGGCACAGGCACAAGCACAGGCTCAAGCACAAGCCCAGGCGCAGGCTCAAGTGCAGGCGCAGGC AGTCGGAGCGCCCACCCCAACTACCTCCAGTCCTGCCCCAGCAGTATCAACTTCCACGCCCACTAGCACA CCCTCCTCAACAACTGCCACCACCACCACAGCCACGAGCGTGGCGCAGACTGTGAGCACCCCCACTACGC AAGATCAGACCCCCTCATCAGCTGTCAGCGTTGCAACTCCTACCGTCTCAGTCAGTGCTCCAGCTCCTAC AGCCACGCCAGTACAGACAGTCCCTCAACCTCACCCCCAGACTCTGCCTCCCGCGGTGCCTCACAGTGTG CCCCAACCCGCTGCAGCTATACCCGCCTTCCCTCCGGTCATGGTTCCCCCATTTCGGGTCCCTCTGCCCG GAATGCCGATCCCCTTGCCAGGAGTAGCAATGATGCAAATCGTTTCTTGTCCATATGTAAAGACTGTCGC TACCACTAAGACCGGAGTGCTGCCTGGCATGGCACCACCAATCGTGCCCATGATCCATCCTCAGGTCGCC ATTGCCGCTTCCCCTGCCACACTCGCTGGTGCCACCGCCGTGAGTGAATGGACCGAATACAAGACGGCTG ATGGTAAGACATATTACTACAATAACCGGACACTGGAATCAACCTGGGAAAAACCTCAGGAGCTCAAGGA GAAAGAAAAACTTGACGAGAAGATCAAAGAACCAATTAAGGAAGCCTCCGAGGAGCCCCTTCCTATGGAG ACTGAAGAGGAAGATCCAAAGGAAGAACCGGTGAAGGAGATCAAGGAGGAGCCTAAGGAGGAGGAGATGA CTGAGGAGGAAAAGGCTGCCCAAAAAGCCAAGCCTGTCGCTACAACACCAATCCCCGGCACGCCTTGGTG CGTGGTCTGGACAGGTGATGAGAGAGTCTTCTTCTACAATCCAACCACTAGGCTCAGTATGTGGGACAGG CCAGACGACCTGATCGGTCGCGCCGACGTTGATAAGATTATCCAGGAGCCACCCCACAAGAAGGGCCTGG AGGATATGAAAAAGTTGAGGCACCCTGCACCGACCATGCTCTCCATACAGAAGTGGCAGTTCTCCATGAG CGCCATAAAGGAGGAGCAGGAACTGATGGAGGAGATGAACGAGGACGAACCTATCAAGGCCAAAAAGCGG AAACGGGACGATAACAAGGATATCGACTCTGAGAAGGAGGCAGCCATGGAGGCCGAAATCAAAGCAGCTC GCGAAAGGGCCATTGTGCCTCTGGAGGCACGGATGAAGCAATTTAAAGACATGCTTCTTGAGAGAGGAGT GTCTGCTTTCAGCACCTGGGAAAAAGAGCTGCACAAAATTGTCTTCGACCCCCGCTATCTGCTGCTCAAC CCAAAAGAAAGGAAGCAAGTGTTTGATCAATACGTGAAAACCAGGGCCGAGGAAGAGAGGAGAGAGAAGA AGAATAAGATTATGCAGGCCAAGGAAGACTTTAAAAAGATGATGGAAGAGGCAAAATTTAATCCCCGAGC CACTTTTTCTGAGTTCGCCGCCAAACACGCGAAAGATAGCAGGTTCAAGGCAATTGAAAAGATGAAGGAC CGCGAGGCCCTTTTTAACGAGTTCGTCGCAGCAGCCAGAAAGAAAGAGAAGGAGGACAGCAAGACTAGAG GCGAAAAAATCAAATCCGATTTCTTCGAGCTTCTGAGCAACCACCACCTCGACTCCCAGAGTCGCTGGAG CAAAGTGAAAGACAAGGTCGAGTCTGACCCTAGGTACAAAGCCGTTGACTCTTCCAGCATGAGAGAGGAC CTGTTTAAGCAGTACATTGAGAAGATTGCTAAAAACCTCGACTCCGAGAAGGAAAAAGAACTGGAGCGCC AGGCCAGGATAGAGGCCAGTTTGAGAGAACGGGAACGGGAGGTGCAGAAGGCCAGGAGTGAGCAGACGAA AGAAATTGACAGAGAACGAGAGCAACACAAACGCGAGGAGGCTATTCAAAATTTTAAGGCACTGCTGAGC GATATGGTGCGAAGCTCAGACGTCTCATGGTCCGACACTCGAAGGACTTTGCGCAAGGACCATCGCTGGG AAAGCGGATCTCTTTTGGAGAGGGAAGAGAAAGAGAAACTGTTTAATGAGCACATCGAAGCCCTTACAAA GAAGAAGAGGGAGCATTTCAGACAGCTGCTTGACGAAACAAGCGCTATTACGTTGACCTCTACCTGGAAA GAGGTGAAAAAAATCATTAAAGAGGACCCTCGCTGTATTAAATTCTCTAGTTCTGACCGCAAGAAGCAAC GGGAGTTCGAAGAGTACATCCGCGATAAGTACATCACTGCTAAAGCAGACTTTAGAACTCTGCTGAAGGA GACAAAATTTATAACTTACCGGTCTAAAAAATTGATTCAAGAGTCAGATCAGCATCTGAAAGACGTTGAG AAGATCTTGCAGAACGACAAACGGTACCTCGTGCTTGATTGCGTGCCAGAAGAGAGGAGGAAACTCATCG TGGCATACGTGGATGACCTGGACCGGCGCGGACCTCCACCACCCCCGACGGCATCCGAACCCACCCGGCG ATCTACCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR217424 representing NM_001039474 Red=Cloning site Green=Tags(s) MAERGGDGGEGERFNPGELRMAQQQALRFRGPAPPPNAVMRGPPPLMRPPPPFGMMRGPPPPPRPPFGRP PFDPNMPPMPPPGGIPPPMGPPHLQRPPFMPPPMGAMPPPPGMMFPPGMPPGTAPGAPALPPTEEIWVEN KTPDGKVYYYNARTRESAWTKPDGVKVIQQSELTPMLAAQAQVQAQAQAQAQAQAQAQAQAQAQAQAQAQ AQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQVQAQAVGAPTPTTSSPAPAVSTSTPTST PSSTTATTTTATSVAQTVSTPTTQDQTPSSAVSVATPTVSVSAPAPTATPVQTVPQPHPQTLPPAVPHSV PQPAAAIPAFPPVMVPPFRVPLPGMPIPLPGVAMMQIVSCPYVKTVATTKTGVLPGMAPPIVPMIHPQVA IAASPATLAGATAVSEWTEYKTADGKTYYYNNRTLESTWEKPQELKEKEKLDEKIKEPIKEASEEPLPME TEEEDPKEEPVKEIKEEPKEEEMTEEEKAAQKAKPVATTPIPGTPWCVVWTGDERVFFYNPTTRLSMWDR PDDLIGRADVDKIIQEPPHKKGLEDMKKLRHPAPTMLSIQKWQFSMSAIKEEQELMEEMNEDEPIKAKKR KRDDNKDIDSEKEAAMEAEIKAARERAIVPLEARMKQFKDMLLERGVSAFSTWEKELHKIVFDPRYLLLN PKERKQVFDQYVKTRAEEERREKKNKIMQAKEDFKKMMEEAKFNPRATFSEFAAKHAKDSRFKAIEKMKD REALFNEFVAAARKKEKEDSKTRGEKIKSDFFELLSNHHLDSQSRWSKVKDKVESDPRYKAVDSSSMRED LFKQYIEKIAKNLDSEKEKELERQARIEASLREREREVQKARSEQTKEIDREREQHKREEAIQNFKALLS DMVRSSDVSWSDTRRTLRKDHRWESGSLLEREEKEKLFNEHIEALTKKKREHFRQLLDETSAITLTSTWK EVKKIIKEDPRCIKFSSSDRKKQREFEEYIRDKYITAKADFRTLLKETKFITYRSKKLIQESDQHLKDVE KILQNDKRYLVLDCVPEERRKLIVAYVDDLDRRGPPPPPTASEPTRRSTK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001039474
ORF Size	3300 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001039474.1, NP_001034563.1
RefSeq Size	4409 bp
RefSeq ORF	3303 bp
Locus ID	56070
UniProt ID	Q8CGF7
MW	123.8 kDa
Gene Summary	Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function]

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