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Negr1 (NM_177274) Mouse Tagged ORF Clone
CAT＃: MR217362
- TrueORF®
Negr1 (Myc-DDK-tagged) - Mouse neuronal growth regulator 1 (Negr1), transcript variant 2
ORF Plasmid: tGFP
"NM_177274" in other vectors (4)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5330422G01Rik; KILON; Ntra |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217362 representing NM_177274
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTCCTGGCGCAGGGCGCTTGTTGCTCGAACCAGTGGCTGGCGGCGGTGCTTCTGAGCCTGTGCT CTTGCCTCCCGGCCGGGCAGAGCGTGGACTTCCCTTGGGCGGCCGTGGACAACATGCTGGTGAGGAAAGG TGACACAGCGGTGCTCAGGTGTTACTTGGAAGATGGAGCATCAAAGGGTGCCTGGCTCAACAGGTCAAGT ATCATTTTTGCTGGAGGTGACAAGTGGTCAGTGGACCCTCGAGTTTCCATTTCCACATTGAATAAAAGAG ACTACAGCCTCCAGATACAGAATGTTGATGTAACAGATGATGGCCCGTACACCTGTTCTGTGCAGACCCA ACACACACCACGGACAATGCAGGTTCATCTCACTGTGCAAGTCCCACCGAAAATATACGACATCTCAAAT GACATGACCATCAATGAAGGAACCAATGTCACCCTTACTTGTTTGGCCACTGGGAAGCCAGAGCCTGTCA TTTCCTGGAGGCATATCTCCCCATCAGCAAAACCATTTGAAAATGGACAATATTTGGACATTTATGGAAT TACAAGGGACCAGGCTGGGGAGTATGAATGCAGTGCAGAAAACGATGTGTCGTTCCCAGACGTGAAGAAA GTGAGAGTGATCGTGAACTTTGCGCCTACAATTCAGGAAATTAAATCTGGCACTGTGACCCCTGGACGCA GTGGACTGATACGATGCGAGGGTGCAGGTGTGCCGCCGCCGGCCTTCGAGTGGTACAAAGGAGAGAAGAG GCTCTTCAATGGCCAACAAGGAATTATCATTCAGAATTTTAGCACAAGATCCATCCTCACAGTGACCAAT GTGACGCAGGAGCACTTCGGCAACTATACTTGTGTGGCTGCCAACAAGTTGGGTACAACCAACGCGAGCC TGCCCCTCAACCAGAGTTCCATTCCCTGGCAAGTTTTCTTCATGTTGAAAGTTTCCTTTCTTCTGGTCTG CATCTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217362 representing NM_177274
Red=Cloning site Green=Tags(s) MVLLAQGACCSNQWLAAVLLSLCSCLPAGQSVDFPWAAVDNMLVRKGDTAVLRCYLEDGASKGAWLNRSS IIFAGGDKWSVDPRVSISTLNKRDYSLQIQNVDVTDDGPYTCSVQTQHTPRTMQVHLTVQVPPKIYDISN DMTINEGTNVTLTCLATGKPEPVISWRHISPSAKPFENGQYLDIYGITRDQAGEYECSAENDVSFPDVKK VRVIVNFAPTIQEIKSGTVTPGRSGLIRCEGAGVPPPAFEWYKGEKRLFNGQQGIIIQNFSTRSILTVTN VTQEHFGNYTCVAANKLGTTNASLPLNQSSIPWQVFFMLKVSFLLVCIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177274 |
| ORF Size | 987 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_177274.4, NP_796248.1 |
| RefSeq Size | 2260 bp |
| RefSeq ORF | 990 bp |
| Locus ID | 320840 |
| UniProt ID | Q80Z24 |
| MW | 36.6 kDa |
| Gene Summary | May be involved in cell-adhesion. May function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC214487 | Negr1 (untagged) - Mouse neuronal growth regulator 1 (Negr1), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
| MG217362 | Negr1 (tGFP-tagged) - Mouse neuronal growth regulator 1 (Negr1) transcript variant 2, (10ug) |
CNY 2,850.00 |
|
| MR217362L3 | Lenti ORF clone of Negr1 (Myc-DDK-tagged) - Mouse neuronal growth regulator 1 (Negr1), transcript variant 2 |
CNY 4,750.00 |
|
| MR217362L4 | Lenti ORF clone of Negr1 (mGFP-tagged) - Mouse neuronal growth regulator 1 (Negr1), transcript variant 2 |
CNY 4,750.00 |
