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Sema3d (NM_028882) Mouse Tagged ORF Clone
CAT＃: MR217350
- TrueORF®
Sema3d (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d)
ORF Plasmid: tGFP
"NM_028882" in other vectors (4)
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CNY 8,544.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4631426B19Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217350 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGTTACTAAAGATGAGAACCCAAGATCCAGAAGTCAAGATCTTCACCTTTTTCATGCTTGGATGA TGTTAATCATGACGGTGCTCTTTCTTCCTGTCACTGAAACGTCTAAACAAAATATTCCAAGACTCAAGCT AACCTACAAAGACTTGCTGCTTTCAAACACCTGTATCCCCTTTTTGGGTTCATCAGAAGGATTGGATTTC CAGACTCTTCTTTTGGATGAGGAGAGGGGCATACTGCTCCTAGGAGCCAAAGACCATGTCTTCCTGCTCA GTCTGGTTGACTTGAACAAGAATTTTAAGAAGATTTATTGGCCTGCTGCAAAAGAACGAGTGGAGCTATG TAAATTAGCTGGGAAAGATGCCAATGCAGAATGTGCAAATTTCATCCGTGTGCTTCAACCCTATAATAAG ACTCACGTTTACGTGTGTGGAACTGGAGCGTTTCATCCGCTGTGTGGGTACATTGATCTCGGCGCCAACA AGGAGGAACTCATATTTAAACTAGACACGCACAACCTGGAGTCTGGCAGACTGAAATGTCCCTTTGATCC TCAGCAGCCTTTTGCTTCAGTAATGACAGATGAGCACCTCTACTCTGGAACAGCTTCTGATTTCCTTGGC AAAGACACTGCATTCACAAGGTCTCTGGGGCTAATGCAGGACCACCATTCCATCAGAACTGACATTTCAG AGCACCACTGGCTCAATGGAGCAAAATTTATCGGAACATTCCCCATTCCAGACACCTATAATCCAGATGA TGATAAAATATATTTCTTCTTTCGAGAATCATCCCAGGAAGGCAGTACTTCTGACAGAAGCATTCTTTCA AGAGTTGGAAGAGTTTGTAAGAATGATGTAGGTGGGCAACGAAGTCTGATAAACAAATGGACAACTTTTC TAAAGGCAAGACTGATTTGCTCGATTCCTGGAAGCGATGGGGCAGATACCCATTTTGATGAACTCCAAGA TATTTACTTACTCCCTACGAGAGATGAAAGAAATCCTGTAGTATATGGAGTCTTTACCACAACCAGCTCC ATCTTCAAAGGCTCTGCTGTCTGTGTGTACAGCATGGCTGATATCCGAGCAGTCTTTAATGGTCCCTATG CTCATAAGGAAAGTGCTGACCATCGCTGGGTGCAATATGATGGAAGGATACCTTACCCCCGACCTGGAAC GTGTCCAAGCAAAACCTATGACCCACTGATTAAGTCCACCCGAGACTTTCCAGACGATGTTATCAGTTTC ATAAGGCGGCACCCTGTGATGTATAAGTCCGTGTACCCAGTGGCAGGAGCACCGACCTTCAAGAGAATCA ACGTGGATTACAGACTGACGCAGATAGTGGTGGATCACGTGGTCGCTGAAGACGGGCAGTATGATGTCAT GTTTCTCGGAACAGACATTGGAACAGTCCTGAAAGTTGTGAGCATCTCCAAGGAGAAGTGGAATATGGAA GAGGTCGTACTGGAGGAGCTTCAGGTATTCAAGCACCCAACAGCTATCTTGAACATGGAGTTGTCGCTGA AGCAGCAACAGTTGTACGTTGGTTCCTGGGATGGATTGGTCCAGCTCTCCTTGCACAGATGCGACACTTA CGGGAAAGCATGTGCAGACTGCTGTCTCGCCAGAGACCCTTACTGTGCCTGGGATGGAAATGCTTGCTCC AGATATGCACCCACGTCTAAAAGGCGAGCTAGACGCCAGGATGTAAAATATGGGGACCCAATCACTCAGT GCTGGGACATAGAAGACAGCATTAGTCATGAAACAGCTGATGAAAAGGTGATTTTTGGAATTGAATTTAA TTCAACCTTTTTGGAGTGTATACCTAAATCCCAACAAGCCTCTGTTGAGTGGTACATCCAGCGGTCAGGA GATGAGCATCGAGAGGAGTTGAAACCTGATGAAAGGATCATCAAAACTGACTATGGGCTACTGATTCGAA GTCTGCAGAAGAAGGATTCTGGGATGTATTACTGCAAAGCACAGGAGCACACTTTCATCCACACCATAGT GAAGCTGACTTTGAATGTCATTGAGAATGAACAGATGGAAAATACCCAGAGGGCAGAATACCAGGAGGGG CAGGTCAAGGATCTGTTGGCTGAGTCACGGTTGAGATACAAAGACTACATCCAAATCCTTAGCAGCCCGA ACTTCAGCCTGGACCAGTACTGTGAGCAGATGTGGTACAAGGAGAAGCGGAGACAGCGCAACAAGGGCAG CCCAAAGTGGAAGCACATGCAGGAAATGAAGAAGAAACGAAATCGACGACATCACAGAGACCTCGATGAG CTCCAGAGATCAGTAGCTACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217350 protein sequence
Red=Cloning site Green=Tags(s) MNVTKDENPRSRSQDLHLFHAWMMLIMTVLFLPVTETSKQNIPRLKLTYKDLLLSNTCIPFLGSSEGLDF QTLLLDEERGILLLGAKDHVFLLSLVDLNKNFKKIYWPAAKERVELCKLAGKDANAECANFIRVLQPYNK THVYVCGTGAFHPLCGYIDLGANKEELIFKLDTHNLESGRLKCPFDPQQPFASVMTDEHLYSGTASDFLG KDTAFTRSLGLMQDHHSIRTDISEHHWLNGAKFIGTFPIPDTYNPDDDKIYFFFRESSQEGSTSDRSILS RVGRVCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTHFDELQDIYLLPTRDERNPVVYGVFTTTSS IFKGSAVCVYSMADIRAVFNGPYAHKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISF IRRHPVMYKSVYPVAGAPTFKRINVDYRLTQIVVDHVVAEDGQYDVMFLGTDIGTVLKVVSISKEKWNME EVVLEELQVFKHPTAILNMELSLKQQQLYVGSWDGLVQLSLHRCDTYGKACADCCLARDPYCAWDGNACS RYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVIFGIEFNSTFLECIPKSQQASVEWYIQRSG DEHREELKPDERIIKTDYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEYQEG QVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQMWYKEKRRQRNKGSPKWKHMQEMKKKRNRRHHRDLDE LQRSVAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028882 |
| ORF Size | 2334 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028882.4, NP_083158.3 |
| RefSeq Size | 6521 bp |
| RefSeq ORF | 2334 bp |
| Locus ID | 108151 |
| UniProt ID | Q8BH34 |
| MW | 89.5 kDa |
| Gene Summary | Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221516 | Sema3d (untagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d), (10ug) |
CNY 8,544.00 |
|
| MG217350 | Sema3d (tGFP-tagged) - Mouse sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3D (Sema3d), (10ug) |
CNY 10,144.00 |
|
| MR217350L3 | Lenti ORF clone of Sema3d (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d) |
CNY 9,690.00 |
|
| MR217350L4 | Lenti ORF clone of Sema3d (mGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d) |
CNY 9,690.00 |
