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Klhl12 (NM_153128) Mouse Tagged ORF Clone
CAT＃: MR217247
- TrueORF®
Klhl12 (Myc-DDK-tagged) - Mouse kelch-like 12 (Drosophila) (Klhl12)
ORF Plasmid: tGFP
"NM_153128" in other vectors (4)
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CNY 6,056.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C3ip1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217247 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGGCATTATGGCCCCTAAAGACATAATGACAAACACTCACGCTAAGTCCATCCTCAACTCCATGA ACTCCCTCAGGAAGAGCAACACCCTCTGTGATGTGACCTTGAGAGTGGAGCAGAAAGACTTCCCTGCGCA TCGGATTGTGCTGGCCGCCTGCAGTGATTATTTCTGTGCCATGTTCACTAGTGAGCTTTCAGAGAAGGGG AAGCCGTATGTTGACATTCAAGGTTTAACTGCTGCTACCATGGAGATCCTGCTGGACTTCGTGTACACAG AAACAGTACATGTGACAGTGGAGAATGTTCAAGAACTGCTCCCTGCAGCCTGTCTTCTTCAGCTGAAAGG TGTGAAACAAGCCTGCTGTGAGTTCTTAGAAAGTCAGCTGGATCCATCTAATTGCCTGGGTATCAGGGAT TTTGCTGAAACTCACAATTGCGTTGACCTGATGCAAGCAGCTGAGGTGTTTAGCCAGAAGCATTTTCCTG AAGTGGTGCAGCACGAGGAGTTCATTCTTCTGAGTCAAGGGGAGGTGGAGAAGCTAATCAAGTGCGACGA GATTCAGGTGGATTCTGAAGAGCCAGTCTTTGAGGCTGTTATCAACTGGGTGAAGCATGCCAAGAAGGAG CGAGAAGAGTCTTTACCTGACCTCTTACAGTATGTTCGGATGCCCCTGCTGACCCCCAGGTACATTACAG ATGTAATTGATGCTGAGCCTTTCATCCGCTGTAGTTTACAATGCAGAGATCTAGTTGATGAAGCAAAGAA GTTTCACCTGAGGCCTGAACTTCGGAGTCAGATGCAAGGACCCAGAACAAGGGCCCGGCTAGGAGCCAAT GAAGTGCTTCTGGTGGTTGGGGGCTTCGGAAGCCAGCAGTCTCCTATTGATGTGGTAGAGAAGTATGACC CCAAGACACAGGAGTGGAGCTTTTTACCAAGTATCACTCGCAAGAGACGGTATGTGGCCTCAGTTTCCTT ACATGATCGGATCTATGTAATTGGTGGCTACGATGGCCGTTCCCGCCTCAGTTCGGTGGAATGTCTAGAC TATACAGCAGACGAAGATGGAGTGTGGTACTCTGTGGCCCCTATGAATGTGCGGCGAGGCCTTGCTGGAG CCACCACTCTGGGAGATATGATTTACGTCTCTGGAGGCTTTGATGGAAGTAGGCGTCATACAAGTATGGA GCGGTATGACCCAAACATCGATCAGTGGAGTATGCTGGGAGATATGCAGACAGCTCGAGAGGGTGCAGGA CTAGTAGTGGCCAGCGGAATAATCTATTGTCTAGGAGGATATGATGGCTTGAACATATTAAATTCAGTTG AGAAATATGATCCCCATACAGGACACTGGACTAACGTTACGCCTATGGCCACCAAGCGTTCTGCTTATAA CATTCGCACTGATTCCTGGACAACTGTCACAAGTATGACCACGCCTCGATGCTATGTAGGGGCCACAGTG CTTCGAGGGAGACTCTATGCAATTGCAGGATACGATGGGAATTCTCTGCTGAGCAGCATTGAGTGTTATG ACCCTATCATCGACAGCTGGGAAGTAGTGGCCTCCATGGGAACCCAGCGTTGTGATGCTGGTGTGTGTGT TCTCCGAGAAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217247 protein sequence
Red=Cloning site Green=Tags(s) MGGIMAPKDIMTNTHAKSILNSMNSLRKSNTLCDVTLRVEQKDFPAHRIVLAACSDYFCAMFTSELSEKG KPYVDIQGLTAATMEILLDFVYTETVHVTVENVQELLPAACLLQLKGVKQACCEFLESQLDPSNCLGIRD FAETHNCVDLMQAAEVFSQKHFPEVVQHEEFILLSQGEVEKLIKCDEIQVDSEEPVFEAVINWVKHAKKE REESLPDLLQYVRMPLLTPRYITDVIDAEPFIRCSLQCRDLVDEAKKFHLRPELRSQMQGPRTRARLGAN EVLLVVGGFGSQQSPIDVVEKYDPKTQEWSFLPSITRKRRYVASVSLHDRIYVIGGYDGRSRLSSVECLD YTADEDGVWYSVAPMNVRRGLAGATTLGDMIYVSGGFDGSRRHTSMERYDPNIDQWSMLGDMQTAREGAG LVVASGIIYCLGGYDGLNILNSVEKYDPHTGHWTNVTPMATKRSAYNIRTDSWTTVTSMTTPRCYVGATV LRGRLYAIAGYDGNSLLSSIECYDPIIDSWEVVASMGTQRCDAGVCVLREK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153128 |
| ORF Size | 1626 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153128.1 |
| RefSeq Size | 3196 bp |
| RefSeq ORF | 1626 bp |
| Locus ID | 240756 |
| UniProt ID | Q8BZM0 |
| MW | 60.6 kDa |
| Gene Summary | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). The BCR(KLHL12) complex is also involved in neural crest specification: in response to cytosolic calcium increase, interacts with the heterodimer formed with PEF1 and PDCD6/ALG-2, leading to bridge together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export. As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4 and PEF1, without leading to degradation of these proteins.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218640 | Klhl12 (untagged) - Mouse kelch-like 12 (Drosophila) (Klhl12), (10ug) |
CNY 6,056.00 |
|
| MG217247 | Klhl12 (tGFP-tagged) - Mouse kelch-like 12 (Drosophila) (Klhl12), (10ug) |
CNY 5,990.00 |
|
| MR217247L3 | Lenti ORF clone of Klhl12 (Myc-DDK-tagged) - Mouse kelch-like 12 (Drosophila) (Klhl12) |
CNY 7,320.00 |
|
| MR217247L4 | Lenti ORF clone of Klhl12 (mGFP-tagged) - Mouse kelch-like 12 (Drosophila) (Klhl12) |
CNY 7,320.00 |
