United States
Germany
Japan
United Kingdom
China
Dusp27 (NM_001033344) Mouse Tagged ORF Clone
CAT＃: MR217222
- TrueORF®
Dusp27 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 27 (putative) (Dusp27), transcript variant 1
ORF Plasmid: tGFP
"NM_001033344" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,928.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C130085G02Rik; Gm209 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217222 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACCGGTGGAGACGCAGAGGAGGAGCAGGTAGTTCCAAACGAGGAGGATGAAGCAGATGTGAGAG CTGTACAGGCCCGCTATCTCCGGAGTCCCTCCCCAAGCCAGTACTCGGTGGTCTCAGAGGCAGAAACCGA AAGCATCTTCATGGAGCCCATCCACCTCTCCTCGGCTGTAGCTGCCAAACAGATCATCAACGAAGAACTC AAGCCTCGGGGGCTCAGAACAGACACTGAGTGTCCAGGCATGCTGGAGTCTGCTGAACAACTGCTGGTGG AAGACCTGTACAACCGTGTCCGTGAGAAGATGGATGACAGGAGCCTGTTCAACACCCCCTGTGTGCTGGA CCTGCAGCGGGCGCTGACCCAGGACCGGCAAGAGGCCCCTCGGAATGAGGTGGATGAGGTGTGGCCCAAC GTCTTCATAGCTGAGAAAAGCGTGGCTGTGAACAAGGGGCGGCTCAAGAGGCTGGGCATCACCCACATTC TGAATGCCGCACATGGCACAGGAGTTTACACTGGTTCTGAATTCTACACTGGCCTGGAGATCCAGTACCT GGGGGTGGAAGTGGATGACTTCCCAGAGGTGGACATCTCCCAACATTTCCGGAAAGCAGCTGAGTTCCTT GACGAAGCTCTGCTGACCTACAGAGGGAAAGTCCTGGTCAGCAGTGAAATGGGTATCAGCAGGTCAGCAG TGCTGGTGGTCGCCTACCTGATGATCTTCCACAGCATGGCCATCCTGGAGGCCTTGATGACTGTGCGCAG AAAGCGAGCTATCTACCCCAATGACGGCTTCCTAAAACAACTGCGGGAGCTCAATGAGAAATTGATGGAG GAGAGGGAAGAGGAGGATGGTGAAGAGGAGTCTGAGGAAGATGCCGGGAGCATGCTGGGTGCTAGAGTCA ATTCACTGATGGTGGAAGAAGAAGATGATGCCACTAGCCACCTGAGTGGCTCCTCCTTGGGGAAGGCCAG CCAGGTCTCCAAACCAGTCACTCTTATTGATGACGAGGAGGAGGAGAAGAAGCTGTATGAAGAGTGGAGG AAGGGGCAGGGCTTCCCCAAGGGGGAGGCTGCTCAGGGTAGAAAGGGACGTAGCTGTTCTATGTCCTCAG CACAGGATGGAGACGACTGTGAGGATGAGGATGTAGAAAGGATAATCCAGGAGTGGCAGAGCCGAAATGA GAGGTACCAAGCCAAAGGGCGCGAGCAGTGGAACCGGGAGGAAGAAGAGGAGGAAGAGAACTCCTACTCC AGCAGAAGGCGCAGACACACCCTAAGCGAGAGCAGTGCTTCTGAGAGTGTGAGCAGCCATGACATCCGGA TCCTGAAGCAGCAACTGGAGAGGAGCACCCAAAGCCGCCGCGGAAGATACCGCTCTGACTCGGAGTCCTC GGAGAGCACCTGGGACATGTGGAACGAGAGGCTGGTGGAGATCGAGAAGGAGGCTGCCCGGAAGTACCGC TCCAAGAGCAAGAGGGAGGAGCTGGATGGCGATTGTTCAGAGGCAGGGGGCAGGGTCCGAGAGGACGATG AGGAGAGCGTGTTGTCTGAGGCCAGCTCCTTCTACAACTTCTGCAGTAGGAACAAGGACAAGCTCACTCC CCTGGAAAGGTGGAAGATTAAGAGAATCCAATTTGGGTTTCACAAGAAAGACTCGGAGGCGGGAGACGGG GGCAGTGAGCATGGTACAGAAGAGGCAGCAGCAGGAGAGAAGAACCTCTCTGATGTCAACCTGACAGCCT ACCAGGCCTGGAAGCTGAAGCACCAGAAAAAGGTGGGGAGTGAGAACAAGGAGGAAGTGGTTGAGATGAG CAAAGGAGAAGACACGGTCTTGGCTAAGAAGAGACAGCGGAGACTAGAGTTACTGGAGAGGAGCAGGCAG ACACTGGAGGAGAGTCAGTCCATGGGAAGCTGGGAGGCAGACAGCTCAACAGCCAGCAGGAGCATCCCCC TGTCTGCATTCTCGTCCGCGGCCCCTTCTGTCAGTGCGGATGGGGACACTGCGTCAGTACTCAGCACCCA GAGCCACCGTTCTCATGCAAGTAACATGCCCGCCACCCCCCTGCCTAACCTGCCGGTGGGGCCTGGAGAC ACCATTTCCATTGCCAGTATCCAGAACTGGATTGCCAATGTCGTCAATGAAACCCTCGCCCAGAAGCAAA ACGAAATGCTCTTGTTGTCCCGCCCACCCTCTGTTGCAAGCATGAAGGCAGCTCCAGCAGCCTGCGGCCT TGGGGGGGATGACCAGCTCTCCGTGCTCAGCACCTCCCTGAGTGGCTGCTTACCACCTCCAAGCCAAGGG AGACCCAGCTCCGACGTGCAGTCTGTGCTGTCCTCCACCAGCTCGCTGACCTCCAGGGCTGAAGGCAGTG GGAACAAAGTGAGGGGGACCAGCAAGCCCATCTACAGCCTCTTTGCTGACAACGTGGACCTGAAGGAGCT TGGCCGGAAGGAGAAAGAGATGCAAATGGAGCTGCAGGAGAAGATGTCCGAGTATAAAATGGAGAAGCTG GCCTCTGATAACAAGCGTAGCTCTCTCTTCAAGAAGAAGAAGGCCAAGGATGACGAGGACATGAGCGTGG GTGACAGAGACGAGGACACAGACAGTGCCATCGGAAGCTTCCGGTACTCCTCTCGCAGTAATTCCCAGAA GCCGGAGACAGATGCCTCCTCTTCTCTGGCCATCTCTGATCACTATAGAAATGGCCGCAGCATGGGTAAC GAGATGGATAGCAATATTAATACGTGGCTCAGTGGCCTCAGGATGGAAGAAAAATCTCCTCCTCAAAGTG ATTGGTCCGGAAGTTCCAGGGGGAGGTATACCAGGTCTTCTCTGCTCCGGGAAACAGAGTCTAAATCCTG CAGTTACAAGTTCTCCAAATCTAGGTCACAGGAGCAGGACACCTCCTTCCACGAAGCCAATGGTGACACT GTGCGAAACACCTCACGGTTCTCATCTTCCACCACCAAGGAGGCCAGAGAGATGCACAAGTTCTCTCGGT CCACATTCAGCGAGACCTCGAGCTCCCGAGAGGAGAGCCCAGAACCCTATTTCTTCCGCCGGACCCCTGA ACCCTCTGATGGGGAGGAGTCCCCAGAGCCAAGGCGTCCAAACTGGACCAGGCCCAGGGACTGGGAAGAT GTAGAAGAGTCATCTAAATCAGACTTCGCTGAGTTTGGGGCCAAGAGGAAATTCACCCAGAGCTTCATGA GGTCTGAAGAGGAGGGAGAGAAGGAGAGGACAGAAAACCGAGAGGAAGGGAGGTTTGCATCTGGGCGTCA GTCCCAGTATCGGAGAAGCACAAACCAGCAAGAGGAAGAAGAAATGGATGATGAAGCCATCATTGCCGCC TGGAGAAAGCGGCAAGAGGAAACCAGAACCAAACTGCAAAGGAGGAGGGAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217222 protein sequence
Red=Cloning site Green=Tags(s) MATGGDAEEEQVVPNEEDEADVRAVQARYLRSPSPSQYSVVSEAETESIFMEPIHLSSAVAAKQIINEEL KPRGLRTDTECPGMLESAEQLLVEDLYNRVREKMDDRSLFNTPCVLDLQRALTQDRQEAPRNEVDEVWPN VFIAEKSVAVNKGRLKRLGITHILNAAHGTGVYTGSEFYTGLEIQYLGVEVDDFPEVDISQHFRKAAEFL DEALLTYRGKVLVSSEMGISRSAVLVVAYLMIFHSMAILEALMTVRRKRAIYPNDGFLKQLRELNEKLME EREEEDGEEESEEDAGSMLGARVNSLMVEEEDDATSHLSGSSLGKASQVSKPVTLIDDEEEEKKLYEEWR KGQGFPKGEAAQGRKGRSCSMSSAQDGDDCEDEDVERIIQEWQSRNERYQAKGREQWNREEEEEEENSYS SRRRRHTLSESSASESVSSHDIRILKQQLERSTQSRRGRYRSDSESSESTWDMWNERLVEIEKEAARKYR SKSKREELDGDCSEAGGRVREDDEESVLSEASSFYNFCSRNKDKLTPLERWKIKRIQFGFHKKDSEAGDG GSEHGTEEAAAGEKNLSDVNLTAYQAWKLKHQKKVGSENKEEVVEMSKGEDTVLAKKRQRRLELLERSRQ TLEESQSMGSWEADSSTASRSIPLSAFSSAAPSVSADGDTASVLSTQSHRSHASNMPATPLPNLPVGPGD TISIASIQNWIANVVNETLAQKQNEMLLLSRPPSVASMKAAPAACGLGGDDQLSVLSTSLSGCLPPPSQG RPSSDVQSVLSSTSSLTSRAEGSGNKVRGTSKPIYSLFADNVDLKELGRKEKEMQMELQEKMSEYKMEKL ASDNKRSSLFKKKKAKDDEDMSVGDRDEDTDSAIGSFRYSSRSNSQKPETDASSSLAISDHYRNGRSMGN EMDSNINTWLSGLRMEEKSPPQSDWSGSSRGRYTRSSLLRETESKSCSYKFSKSRSQEQDTSFHEANGDT VRNTSRFSSSTTKEAREMHKFSRSTFSETSSSREESPEPYFFRRTPEPSDGEESPEPRRPNWTRPRDWED VEESSKSDFAEFGAKRKFTQSFMRSEEEGEKERTENREEGRFASGRQSQYRRSTNQQEEEEMDDEAIIAA WRKRQEETRTKLQRRRED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033344 |
| ORF Size | 3417 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033344.3, NP_001028516.2 |
| RefSeq Size | 4042 bp |
| RefSeq ORF | 3417 bp |
| Locus ID | 240892 |
| UniProt ID | Q148W8 |
| MW | 128.6 kDa |
| Gene Summary | May be required for myofiber maturation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223720 | Dusp27 (untagged) - Mouse dual specificity phosphatase 27 (putative) (Dusp27), transcript variant 1, (10ug) |
CNY 14,730.00 |
|
| MG217222 | Dusp27 (tGFP-tagged) - Mouse dual specificity phosphatase 27 (putative) (Dusp27) transcript variant 1, (10ug) |
CNY 11,020.00 |
|
| MR217222L3 | Lenti ORF clone of Dusp27 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 27 (putative) (Dusp27), transcript variant 1 |
CNY 11,880.00 |
|
| MR217222L4 | Lenti ORF clone of Dusp27 (mGFP-tagged) - Mouse dual specificity phosphatase 27 (putative) (Dusp27), transcript variant 1 |
CNY 11,880.00 |
