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Gemin5 (NM_001166670) Mouse Tagged ORF Clone
CAT＃: MR217219
- TrueORF®
Gemin5 (Myc-DDK-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 3
ORF Plasmid: tGFP
"NM_001166670" in other vectors (4)
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CNY 15,010.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA407055; AA407208; AI451603; BB194447; C330013N08 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217219 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCGGAGCCGCGGACGCTGCCGCCGTCCCCCAACTGGTACTGCTCACGCTGCAGCGACGCGGCGC CCGGAGGCATCTTTGGCTTCGCTGCGCGGACCTCCGTCTTCCTCGTCCGCGTGGGTCCGGGTGCGGGCGC GAGCCCAGGGGCGCCCCCGTTTCGAGTGGTAGGAGAGTTGGTGGGACACACTGAAAGAGTATCTGGTTTC ACTTTTTCTCATCACCCTGGACAATACAACCTCTGTGCCACCAGCTCAGACGATGGAACTGTGAAGGTTT GGGATGTAGAGACCAAGACTGTAGTGACAGAACACACACTCCATCAGCATACAATATCAGCACTGCATTG GTCTCCGACAGTTAAGGACTTAATAGTCTCTGGGGATGAAAAAGGAGTCGTTTTCTGTTACTGGCTTAAC AGAAATGACAGCCAGCACCTCTTCACAGAGCCCAGGACCATATTCTGTCTGACGTGTTCACCTCATCACG AAAATCTAGTAGCCATTGGCTACAAAGATGGCATAGTGGTTATCATTGACATCAGTAAAAAGGGAGAAGT TATTCACAGACTTCGAGGCCACGATGACGAGATCCACTCCATAGCCTGGTGTCCTCTGTCTGGTGAAGAC TGCTTATCCATCAGCCAAGAGGAAAACTCGGAACCTGACATTCCCAATGGGAAGCTTATAGCAGAAACCC CCATCACAAAAGGCTGCTACTTAGCCACAGGAAGCAAAGATCAGACCATTCGAATCTGGAGCTGTTCCCG CGGCCGGGGTGTAATGGTTTTGAAATTGCCCTTCCTGAAGAGAAGAAGTGGGGGTGTGGACCCAACAGTT AAAGAACGGCTTTGGTTGACACTTCATTGGCCTAAGAACCAACCAACACAGCTGGTATCCAGTTGTTTTG GAGGTGAGCTGCTGCTATGGGACCTCACCCAGTCATGGAGGCGGAAGTACACGCTCTTCAGCACCTCAGC AGAAGGGCACAATCATTCCAGAATTGTCTTCAATCTGTGTTCTCTGAAGACTGAAGATGGCAAGCAGCTG CTACTGTCCACATCCATGGATAGAGATGTAAAATGTTGGGACATGGCCACCCTGGAGTGTTGCTGGACAT TGCCTTCTCTTGGAGGTTTTGCCTACAGCCTGGCCTTTTCTCCTGTGGACGTGGGCTCCCTGGCCATAGG TGTTGGGGACGGCATGATCCGGGTGTGGAATACACTCTCCATAAAGAACAACTACGACGTGAAAAACTTC TGGCAAGGAGTCAAGTCCAAGGTCACAGCGCTATGCTGGCACCCAAACAAGGAAGGCTGCTTAGCCTTCG GGACCGATGACGGAAAAGTGGGATTGTATGACACCTGCTCCAACAAACCTCCACAGATTTCAAGCACGTA TCATAAGAAGACCGTGTATAGGTTGGCTTGGGGGCCACCAGTCCCCCCCATGTCACTTGGAGGGGAAGGA GACAGGCCTTCCCTCACCTTGTACAGCTGTGGAGGGGAAGGCGTTGTGCTACAGCATAACCCCTGGAAGC TCAGTGGAGAGGCCTTCGACATCAACAAACTCGTGAGGGACACCAACTCCATTAGATACAAACTGCCCGT GCACACAGAGATCAGCTGGAAAGGAGATGGCAAAGTCCTGGCTCTTGGGAATGAAGATGGATCAATAGAA ATATTCCAAGTCCCCAACTTGAGGCTGCTGTGCACCATCCAGCAGCACCACAAGCTTGTGAACGCCATAG TCTGGCACCATGAGCATGGCAGCCGTCCAGAGCTGAGCTGTCTCCTTGCTTCTGGCTCCAACAATGCAGT CATCTACGTGCACAACCTGAAAGCAGTCCTAGAAAGCAATCCTGAGTCTCCAATAACCATCACAGAGCCC TACCGCACCCTCTCGGGGCACACAGCCAAGATTACCAGTCTGGCATGGAGCCCACATCATGACGGAAGGC TGGTATCTGCTTGCTACGATGGCACAGCTCAGGTGTGGGATGCCCTCCGGGAAGAACCTTTGTTCAATTT CCGAGGACATCGAGGCCGGCTGCTATGTGTCGCGTGGTCCCCAGTGGATCCAGAATGCATCTACTCAGGT GCAGATGACTTCTGTGTCTACAGGTGGCTGACTTCCATGCAGGACCATTCCCGGCCTCCTCAAGGGAAAA AATGTATTGAACTAGAGAAAAAACGACTCTCTCAATTTAAGCCAAAGCTTAAAAAGAAGAAAAAACCTAC CTTGCGACTGCCTGTAAAGCAGGATTCATCCGTTGGCAATGAAGATGAGAGTGTAAAGGAGAACTCTGGA CCTGCTGAGAACGGCTTGTCGGACCAGGATGGTGAGGAGGAAGCCCAGGAGCCAGAGCTGCCTCCCTCTC CTGTAGTTTGTGTAGAACCTGTTTCCTGTACTGACATTTGCTCAGGCTTTGAAAAGTCAAAAGTCACTGT TAGTAGCAAAGCCACCTCACTGAAAAAGGAGCCAGCTAAAGAGAAACCAGAAGCCTTGCTCAAGAAGAGG AAGGCTCGCTCCATGCTCCCTCTGAGCACCAGCTTAGACCACAGGTCCAAAGAGGAGCTCCATCGAGACT GCTTGGTACTAGCAACTGCAACACACGCCAAAGCAGAGCTAAATGAAGATGTGTCTGCCGACCTTGAGGA ACGATTCCACTTGGGCCTTTTCACAGACAGGGCTACTCTATACAGGATGATGGAAACAGAAGGGAAAGGT CACTTGGAAAGTGGCCACCCCGAGCTTTTTCACCAGCTCATGCTCTGGAAAGGAGATCTGAAGGGTGTTC TCCAGGCTGCAGCAGAGAGAGGCGAGCTGACAGACAGTCTCGTGGCTGTGGCACCAGTGGCTGGCTACAG TGTATGGCTGTGGGCTGTGGAAGCCTTTGCCAAGCAGCTGTGTTTCCAGGACCAGTACGTCAAGGCTGCC TCTTATCTGCTATCCATCCACAAAGTGTACGAAGCTGTGGAACTTCTCAAGTCAAACCATCTCTACAGAG AAGCTATTGCAGTTGCCAAGGCTCGGCTGCGCCCTGAGGACCCTGTTCTGAAGGAACTATACCTCAGCTG GGGGTCCATCCTGGAGAGAGATGGCCACTATGCTATTGCAGCCAAGTGCTACTTAGGAGCCACTTCCGCC TATGATGCAGCCAAAGTTCTGGCCAGAAAGGGAGATGCAGCCTCACTTAGAACAGCTGCAGAGCTGGCTG CCATAGCAGGAGAACATGAGCTGGCTGCTTCCCTGGCTCTTAGATGTGCCCAAGAGCTGCTTCTGATGAA GAACTGGGTGGGTGCACAAGAAGCATTGGGACTGCATGAAAGCCTGCAGGGCCAGAGACTGGTCTTCTGC CTCCTTGAACTTCTATGCCGGCACCTGGAGGAAAAACAGCCTCTAGAGGTCAGAGGCCCCTCTTCCATTT ACCACCAGTGGGCCACAGGTTCTGAGGGAACCTTGGTGCAGAGAGTAACCGGTGTGTGGCGCAGCGCCTT CAGTGTGGACACCCCGGAGCAGTGTCAGGCCGCCTTACAGAAGCTGCAGGATGTCAAGTACCCATCAGCA ACAAGTAACACTCCCTTCAGACAGCTGCTGCTTCATGTCTGCCATGACCTGACCTTGGCAATGCTGAGCC AGCAGGCTGCTGCCTGGGAGGAGGCAGTGCCTGCTCTGCTGCAGGCTGTGGTTCGGAGCTATACCTCAGG GAACTTCACCCTCATGCAGGAAATCTACTCAGCCTTTCTCCCAGGTGGCTGTGACCACCTACGAGACAAA CTGGGTGACCTCTCTCCTGCCATGGCAGCTTACAAAAGCTTAGAGGCCTTTTGTATTTATGGGCAACTGT ATGAAGTCTGGTGGTCGCTCTGCGGGCCTGGCCCTGAGTCAAGCGTCTGGGTGTTGTCAGCTGAAAGCAC GGTCTCTGATAAACAGAGCAAGCCAGAGGACAGTGCCAGTGCTGAGGACATGGAACAGCCTCCAGGCCCG GGCCCGAGGCTCAGTGCAGAGAGTGAGCGTTTGCTGAGTGCCTGCAAGGAGCTTTTCTCAGAACGGCATG CCAGCCTGCAGACCTCCCAGAGGACTGTAGCCGAAGTCCAAGAGACACTGGCAGAAATGATCCGCCAGCA CCAAAAGAGTCAGCTCTGCAAGGCCACAACAAATGGTCCCAGCAGAGATGAACCCAGCAGAGATGAACCC AGCCAGGAAGCAGAGCGGGCTCCTTCTCAGCCTCCGAGCCCAACAGAAGAAAGAAATGCACCAGTGTCTC TCCCTGAACTAACCAGAAGGCTCACAGAGGCAAATGAGAGGATAGCAGAGTTTCCAGAGAGTGTAAAGGC CTGGCCCTTTCCAGATGTGCTGGAGTGCTGTCTCGTCTTGCTTCACATTGGGTCCCAGTGTCCTGACGCT GTGGACCCTGAGATGCAGCAACAGGCCCAGGAGCTCCTTCACAAGTATGGGCACACTCGAGCTTACAGAA GACACTGCCAGAGTCGGCACACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217219 protein sequence
Red=Cloning site Green=Tags(s) MKPEPRTLPPSPNWYCSRCSDAAPGGIFGFAARTSVFLVRVGPGAGASPGAPPFRVVGELVGHTERVSGF TFSHHPGQYNLCATSSDDGTVKVWDVETKTVVTEHTLHQHTISALHWSPTVKDLIVSGDEKGVVFCYWLN RNDSQHLFTEPRTIFCLTCSPHHENLVAIGYKDGIVVIIDISKKGEVIHRLRGHDDEIHSIAWCPLSGED CLSISQEENSEPDIPNGKLIAETPITKGCYLATGSKDQTIRIWSCSRGRGVMVLKLPFLKRRSGGVDPTV KERLWLTLHWPKNQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSTSAEGHNHSRIVFNLCSLKTEDGKQL LLSTSMDRDVKCWDMATLECCWTLPSLGGFAYSLAFSPVDVGSLAIGVGDGMIRVWNTLSIKNNYDVKNF WQGVKSKVTALCWHPNKEGCLAFGTDDGKVGLYDTCSNKPPQISSTYHKKTVYRLAWGPPVPPMSLGGEG DRPSLTLYSCGGEGVVLQHNPWKLSGEAFDINKLVRDTNSIRYKLPVHTEISWKGDGKVLALGNEDGSIE IFQVPNLRLLCTIQQHHKLVNAIVWHHEHGSRPELSCLLASGSNNAVIYVHNLKAVLESNPESPITITEP YRTLSGHTAKITSLAWSPHHDGRLVSACYDGTAQVWDALREEPLFNFRGHRGRLLCVAWSPVDPECIYSG ADDFCVYRWLTSMQDHSRPPQGKKCIELEKKRLSQFKPKLKKKKKPTLRLPVKQDSSVGNEDESVKENSG PAENGLSDQDGEEEAQEPELPPSPVVCVEPVSCTDICSGFEKSKVTVSSKATSLKKEPAKEKPEALLKKR KARSMLPLSTSLDHRSKEELHRDCLVLATATHAKAELNEDVSADLEERFHLGLFTDRATLYRMMETEGKG HLESGHPELFHQLMLWKGDLKGVLQAAAERGELTDSLVAVAPVAGYSVWLWAVEAFAKQLCFQDQYVKAA SYLLSIHKVYEAVELLKSNHLYREAIAVAKARLRPEDPVLKELYLSWGSILERDGHYAIAAKCYLGATSA YDAAKVLARKGDAASLRTAAELAAIAGEHELAASLALRCAQELLLMKNWVGAQEALGLHESLQGQRLVFC LLELLCRHLEEKQPLEVRGPSSIYHQWATGSEGTLVQRVTGVWRSAFSVDTPEQCQAALQKLQDVKYPSA TSNTPFRQLLLHVCHDLTLAMLSQQAAAWEEAVPALLQAVVRSYTSGNFTLMQEIYSAFLPGGCDHLRDK LGDLSPAMAAYKSLEAFCIYGQLYEVWWSLCGPGPESSVWVLSAESTVSDKQSKPEDSASAEDMEQPPGP GPRLSAESERLLSACKELFSERHASLQTSQRTVAEVQETLAEMIRQHQKSQLCKATTNGPSRDEPSRDEP SQEAERAPSQPPSPTEERNAPVSLPELTRRLTEANERIAEFPESVKAWPFPDVLECCLVLLHIGSQCPDA VDPEMQQQAQELLHKYGHTRAYRRHCQSRHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001166670 |
| ORF Size | 4503 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 6223 bp |
| RefSeq ORF | 4506 bp |
| Locus ID | 216766 |
| UniProt ID | Q8BX17 |
| MW | 166.5 kDa |
| Gene Summary | Required for the assembly of the SMN complex that plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex. Binds to the 7-methylguanosine cap of RNA molecules (By similarity). Binds to the 3' UTR of SMN1 mRNA and regulates its translation; does not affect mRNA stability (PubMed:25911097). May play a role in the regulation of protein synthesis via its interaction with ribosomes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224602 | Gemin5 (untagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 3, (10ug) |
CNY 19,190.00 |
|
| MG217219 | Gemin5 (tGFP-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5) transcript variant 3, (10ug) |
CNY 16,530.00 |
|
| MR217219L3 | Lenti ORF clone of Gemin5 (Myc-DDK-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 3 |
CNY 16,910.00 |
|
| MR217219L4 | Lenti ORF clone of Gemin5 (mGFP-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 3 |
CNY 16,910.00 |
