Sema4b (NM_013659) Mouse Tagged ORF Clone

CAT＃: MR217141

  • TrueORF®

Sema4b (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b)

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_013659" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,800.00

货期*
现货
规格
    • 10 ug

Product images

经常一起买 (4)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms mKIAA1745; Semac; SemC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR217141 representing NM_013659
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCGGGCGAGCCGCTCCGCTGTCCTGCGGCGCGCGCTGCTGCTGCTGCTGCTACTGCTGCTGCTGC
GGACGACGACTACGCGGGCGCTCGGCCCCCGGATCAGTGTGCCGCTGGGCTCTGAAGAAAGGCTGATTAG
AAAATTTGAAGCTGAAAACATCTCCAACTACACGGCCCTTCTGCTGAGCCAGGATGGCAAGACGCTGTAT
GTGGGGGCCCGAGAGGCCCTCTTTGCACTTAACAGCAACCTCAGCTTCTTGCCAGGCGGGGAGTACCAAG
AGCTGCTGTGGAGTGCAGATGCTGACAGGAAGCAGCAGTGCAGCTTCAAGGGCAAGGACCCAAAGCGTGA
CTGTCAAAACTACATCAAGATCCTCCTGCCACTCAACAGCAGCCACCTGCTCACCTGTGGCACGGCCGCC
TTCAGCCCCCTGTGTGCTTACATTCACATAGCGAGCTTTACTTTAGCCCAAGATGAGGCCGGCAATGTCA
TTCTGGAGGATGGCAAGGGTCGTTGTCCCTTTGACCCCAACTTCAAGTCCACGGCTCTGGTGGTTGATGG
TGAGCTGTACACTGGAACAGTCAGTAGCTTCCAGGGAAACGACCCAGCCATTTCCCGGAGCCAGAGTTCC
CGCCCCACCAAGACTGAGAGCTCCCTCAACTGGCTACAAGACCCTGCCTTTGTGGCCTCGGCCTACGTCC
CCGAGAGCCTGGGCAGCCCCATAGGTGATGATGATAAGATCTACTTCTTCTTCAGCGAGACGGGCCAGGA
GTTTGAGTTCTTTGAGAACACCATCGTGTCCCGAGTTGCCCGAGTCTGTAAGGGCGATGAGGGTGGAGAG
CGGGTGTTGCAGCAACGCTGGACCTCCTTTCTCAAGGCTCAGCTCCTGTGCTCCCGGCCTGATGATGGCT
TTCCCTTTAACGTGCTACAAGATGTCTTCACCCTGAACCCCAACCCTCAGGATTGGCGCAAGACCCTTTT
CTATGGGGTCTTTACCTCCCAGTGGCACAGAGGGACCACAGAAGGCTCTGCCATCTGCGTCTTCACCATG
AATGATGTGCAGAAGGCCTTTGACGGCCTGTACAAGAAAGTAAACAGAGAGACACAGCAGTGGTATACCG
AGACCCACCAGGTGCCCACACCGCGGCCGGGAGCGTGCATTACCAACAGTGCCCGGGAACGGAAGATCAA
CTCGTCCCTGCAGCTCCCAGACCGAGTGCTGAACTTCCTCAAGGATCACTTCTTGATGGATGGGCAGGTC
CGCAGTCGCCTGCTGCTGCTGCAGCCCAGAGCCCGCTACCAGCGTGTGGCTGTGCACCGTGTGCCTGGCC
TGCACAGCACTTATGATGTCCTATTTCTGGGCACTGGTGATGGCCGCCTGCACAAAGCAGTGACCCTGAG
CTCCAGAGTCCACATCATTGAGGAGCTGCAGATCTTCCCTCAAGGACAGCCTGTGCAGAACCTGCTCTTG
GACAGCCATGGGGGACTGTTGTATGCCTCCTCCCATTCCGGGGTGGTGCAAGTGCCCGTAGCCAACTGCA
GCCTGTACCCAACCTGTGGAGACTGCCTCCTGGCTCGAGACCCCTACTGCGCCTGGACTGGCTCTGCCTG
CAGGCTCGCTAGCCTCTACCAGCCTGATCTGGCCTCCAGGCCATGGACCCAGGACATTGAGGGTGCCAGT
GTCAAGGAACTCTGCAAGAATTCCTCATACAAGGCCCGGTTTCTTGTGCCAGGTAAGCCATGTAAACAAG
TCCAGATCCAACCAAACACAGTGAACACCCTGGCCTGCCCACTCCTCTCAAACCTGGCCACTCGGCTCTG
GGTGCACAATGGAGCCCCAGTCAATGCCTCTGCCTCCTGCCGCGTGTTACCCACCGGGGACCTGCTGCTG
GTGGGCAGCCAGCAGGGTTTGGGGGTGTTCCAGTGTTGGTCGATAGAAGAAGGATTCCAGCAGCTTGTGG
CCAGCTACTGCCCAGAGGTGATGGAGGAGGGGGTAATGGACCAAAAGAACCAGCGTGATGGTACCCCAGT
CATTATCAACACATCACGAGTGAGTGCACCGGCTGGTGGCAGGGCCAGCTGGGGTGCGGACAAGTCCTAC
TGGAATGAATTCCTGGTGATGTGTACTCTGTTTGTGTTTGCTATGGTGCTTTTGTTTCTGTTCTTTCTCT
ACCGACATCGGGATGGCATGAAACTCTTCCTAAAGCAGGGCGAATGTGCCAGTGTGCACCCCAAGACTCG
CCCTATAGTGCTACCACCTGAGACCCGACCGCTGAATGGTGTCGGCCCTCCTAGCACCCCACTTGACCAC
CGAGGCTACCAGGCTCTGTCGGATAGCTCCCCAGGGCCCAGAGTCTTCACTGAATCAGAGAAGAGGCCAC
TGAGCATCCAGGACAGCTTTGTAGAGGTGTCTCCCGTGTGTCCCCGGCCCCGAGTTCGACTGGGCTCTGA
GATCCGAGACTCTGTGGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>MR217141 representing NM_013659
Red=Cloning site Green=Tags(s)

MGRASRSAVLRRALLLLLLLLLLRTTTTRALGPRISVPLGSEERLIRKFEAENISNYTALLLSQDGKTLY
VGAREALFALNSNLSFLPGGEYQELLWSADADRKQQCSFKGKDPKRDCQNYIKILLPLNSSHLLTCGTAA
FSPLCAYIHIASFTLAQDEAGNVILEDGKGRCPFDPNFKSTALVVDGELYTGTVSSFQGNDPAISRSQSS
RPTKTESSLNWLQDPAFVASAYVPESLGSPIGDDDKIYFFFSETGQEFEFFENTIVSRVARVCKGDEGGE
RVLQQRWTSFLKAQLLCSRPDDGFPFNVLQDVFTLNPNPQDWRKTLFYGVFTSQWHRGTTEGSAICVFTM
NDVQKAFDGLYKKVNRETQQWYTETHQVPTPRPGACITNSARERKINSSLQLPDRVLNFLKDHFLMDGQV
RSRLLLLQPRARYQRVAVHRVPGLHSTYDVLFLGTGDGRLHKAVTLSSRVHIIEELQIFPQGQPVQNLLL
DSHGGLLYASSHSGVVQVPVANCSLYPTCGDCLLARDPYCAWTGSACRLASLYQPDLASRPWTQDIEGAS
VKELCKNSSYKARFLVPGKPCKQVQIQPNTVNTLACPLLSNLATRLWVHNGAPVNASASCRVLPTGDLLL
VGSQQGLGVFQCWSIEEGFQQLVASYCPEVMEEGVMDQKNQRDGTPVIINTSRVSAPAGGRASWGADKSY
WNEFLVMCTLFVFAMVLLFLFFLYRHRDGMKLFLKQGECASVHPKTRPIVLPPETRPLNGVGPPSTPLDH
RGYQALSDSSPGPRVFTESEKRPLSIQDSFVEVSPVCPRPRVRLGSEIRDSVV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_013659
ORF Size 2469 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_013659.5
RefSeq Size 3946 bp
RefSeq ORF 2472 bp
Locus ID 20352
UniProt ID Q62179
MW 91.8 kDa
Gene Summary Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
MC221863 Sema4b (untagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), (10ug)
    • 10 ug

CNY 6,032.00

MG217141 Sema4b (tGFP-tagged) - Mouse sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4B (Sema4b), (10ug)
    • 10 ug

CNY 6,370.00

MR217141L3 Lenti ORF clone of Sema4b (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b)
    • 10 ug

CNY 7,700.00

MR217141L4 Lenti ORF clone of Sema4b (mGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b)
    • 10 ug

CNY 7,700.00

You may also need
plasmid icon Empty Vector
protein iconDDK/Flag Antibody

其它Sema4b产品

Expression Plasmids
Lentiviral Particles
CRISPR
RNAi
Proteins
Antibodies
相关产品 (9)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

    • 10 ug

CNY 5,420.00

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

    • 1 ml in 1 vial

CNY 4,090.00

TA50011-100

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

【特别福利】购一抗正装产品，免费领10ul抗体试用装

    • 100 ul

CNY 1,050.00

MG217141

Sema4b (tGFP-tagged) - Mouse sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4B (Sema4b), (10ug)

    • 10 ug

CNY 6,370.00

MC221863

Sema4b (untagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), (10ug)

    • 10 ug

CNY 6,032.00

MR217141L3V

Lenti ORF particles, Sema4b (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), 200ul, >10^7 TU/mL

    • 200 ul

CNY 13,205.00 暂停销售

MR217141L3

Lenti ORF clone of Sema4b (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b)

    • 10 ug

CNY 7,700.00

MR217141L4

Lenti ORF clone of Sema4b (mGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b)

    • 10 ug

CNY 7,700.00

MR217141L4V

Lenti ORF particles, Sema4b (GFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), 200ul, >10^7 TU/mL

    • 200 ul

CNY 14,725.00 暂停销售

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