Ckap5 (NM_001165989) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	3110043H24Rik; 4930432B04Rik; D730027C18Rik; mKIAA0097; T25636
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR217121 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGATGACAGTGAGTGGTTGAAATTACCAGTTGATCAGAAATGTGAACACAAGCTATGGAAAGCAA GATTAAGTGGGTATGAAGAAGCCTTGAAGATCTTCCAGAAAATAAAGGATGAAAAGAGTCCAGAATGGTC CAAATACTTAGGATTAATCAAAAAATTTGTCACTGATTCCAATGCAGTGGTTCAATTGAAAGGACTAGAA GCTGCACTTGTCTATGTTGAAAATGCTCATGTTGCAGGAAAAACAACGGGAGAGGTTGTATCAGGTGTTG TAAGTAAAGTCTTCAACCAACCTAAAGCCAAAGCCAAGGAACTGGGCATAGAGATTTGCCTCATGTATGT TGAGATTGAAAAAGGAGAGTCTGTACAAGAAGAGCTTCTGAAGGGCCTGGATAACAAGAACCCCAAGATC ATAGTGGCCTGTATAGAAACACTGAGGAAAGCCTTAAGTGAATTTGGTTCCAAAATCATCTCACTTAAGC CAATTATTAAAGTGTTGCCAAAACTATTTGAATCTCGAGACAAGGCTGTTCGGGATGAAGCCAAATTATT TGCTATAGAGATTTATCGATGGAATCGAGATGCTGTGAAACACACACTACAAAATATAAATTCTGTCCAG TTGAAAGAATTAGAAGAAGAGTGGGTCAAGCTGCCAACAGGTGCTCCTAAGCCCAGTCGGTTTCTGCGTT CCCAACAAGAACTAGAAGCTAAACTGGAACAGCAACAGTCTGCTGGTGGAGATGCAGAAGGGGGTGGTGA TGATGGTGATGAGGTACCACAGGTAGATGCTTATGAGCTTCTGGATGCGGTAGAGATCCTTTCCAAACTT CCCAAAGACTTTTATGACAAAATTGAGGCAAAGAAATGGCAAGAGAGAAAAGAAGCTCTGGAGGCTGTAG AAGTACTAGTGAAAAACCCCAAGCTGGAAGCAGGCGATTACGCAGATTTAGTGAAAGCATTAAAAAAGGT TGTTGGAAAGGACACCAATGTCATGTTGGTAGCTTTGGCAGCAAAATGTCTTACTGGCTTAGCTGTTGGG CTCAGAAAGAAATTTGGACAATATGCAGGACATGTTGTGCCAACAATTTTGGAAAAATTCAAAGAAAAGA AGCCTCAAGTGGTACAAGCCCTGCAGGAGGCAATTGATGCAATCTTCCTCACTACCACACTACAGAACAT CAGTGAGGATGTCTTAGCAGTAATGGATAATAAAAATCCCACCATCAAGCAGCAGACATCACTTTTTATT GCAAGAAGCTTTCGTCACTGCACTTCCTCTACATTGCCGAAGAGTCTACTAAAACCCTTTTGTGCTGCTC TACTTAAGCACATCAATGATTCTGCTCCTGAAGTCAGAGATGCTGCATTTGAAGCACTGGGTACAGCTTT GAAGGTGGTTGGTGAGAAATCAGTAAACCCATTTTTAGCTGATGTTGACAAACTCAAACTTGATAGGATC AAAGAATGTTCAGAAAAAGTAGAACTGGTTCATGGTAAAAAATCTGGACTTGCAACTGAAAAGAAGGAAT CCAAGCCTCTACCTGGAAGGGCTGCAGCATCAGGGGCTGCAGGGGACAAAGACACAAAAGATGTTTCAGG ACCCAAACCAGGACCGTTAAAAAAAACACCTACTGCTAAGGCTGGTGGACCATCTAAGAAAGGTAAAACA ACTGCACCAGGTGGCTCAGCAAGTGCCGGGACAAAGAATAAGAAAGGATTGGAGACCAAAGAAATAGTGG AGCCAGAACTCTCAATTGAAGTATGTGAAGAGAAAGCTTCAGCTGTTCTTCCCCCAACCTGTATTCAGCT TCTAGACAGCAGCAACTGGAAGGAAAGGCTGGCCTGTATGGAAGAATTCCAAAAGGCTGTTGAACTAATG GAGCGAACTGAGATGCCGTGCCAGGCATTGGTGAAGATGCTAGCAAAAAAACCTGGATGGAAAGAAACTA ATTTTCAGGTGATGCAGATGAAGCTTCACATTGTTGCTTTGATTGCCCAGAAGGGAAATTTTTCTAAAAC GTCTGCTCAGATTGTATTAGATGGCCTTGTAGACAAGATTGGAGATGTGAAATGTGGGAACAATGCAAAA GAAGCCATGACAGCAATAGCTGAAGCGTGCATGCTGCCCTGGACAGCTGAGCAGGTGATGTCAATGGCCT TCTCACAAAAGAATCCTAAGAATCAATCAGAAACTCTAAATTGGCTATCAAATGCAATAAAAGAATTTGG ATTTTCTGAGTTGAATGTCAAAGCTTTCATTAGCAACGTGAAGACTGCTCTTGCTGCAACAAATCCTGCT GTGAGGACTTCTGCCATCACCCTGCTTGGCGTGATGTATCTATATGTTGGTCCTTCTTTGAGAATGATCT TTGAGGATGAGAAGCCTGCCCTCCTATCCCAGATAGATGCAGAATTTCAAAAGATGCAGGGACAAAGTCC TCCCGCTCCAACGAGGGGAATTGCTAAGCACAGCACAAGTGCTACAGATGAAGGAGAAGATGGAGAGGAA CCAGGTGAAGGGGGCAATGATGTTGTTGATCTTTTGCCGAGGATTGAGATCAGTGACAAAATTACTTCAG AGTTGGTGTCTAAGATTGGTGACAAGAATTGGAAGATCAGGAAAGAAGGCCTAGATGAAGTGGCAGGTAT TATCAATGAAGCAAAATTTATCCAACCAAATATAGGTGAACTTCCAACTGCCTTGAAGGGTCGACTGAAT GATTCAAACAAAATCTTGGTGCAGCAGACACTGAATATCCTTCAGCAACTGGCAGTAGCCATGGGTGCAA ACATCAGGCAGCATGTAAAGAACCTGGGCATCCCTGTCATTACTGTTCTCGGAGATAGCAAGAACAATGT TCGAGCTGCTGCCCTAGCAACTGTGAATGCTTGGGCAGAACAAACTGGCATGAAAGAATGGCTGGAGGGA GAAGATCTTTCTGAAGAACTTAAAAAGGAAAATCCCTTCCTGAGGCAAGAGCTTCTGGGATGGCTGGCAG AGAAACTACCTACACTCCGTTCCACTCCTACAGATCTTATCCTTTGTGTTCCTCATCTGTATTCCTGTCT AGAAGATCGAAATGGAGATGTACGGAAGAAGGCCCAAGATGCTTTGCCATTCTTTATGATGCATTTAGGA TATGAAAAAATGGCTAAGGCTACTGGGAAACTAAAGCCAACTTCTAAAGATCAAGTACTGGCCATGCTAG AAAAGGCCAAAGCTAACATGCCATCCAAGCCTGCTGCACCTGCTAAGGCCATGTCCAAACCAATGGGAGG GTCGGCTCCAGCCAAAACCCAGCCTATACCAGCACCTGTTGAAGATTCTGTTTCCAGTACCATAGAAGCT AAGCCTGATCTGAAAAAGGCTAAAGCTCCAGGCGTTTCTTCCAAAGCAAAGAGTGTACAAGGGAAGAAAG TGCCAAGCAAAACCACCTTAAAGGAAGACGACGACAAATCTGGTCCTATTTTTATTGTTGTTCCAAATGG AAAGGAGCAAAGGATGAGAGATGAGAAAGGATTAAAGGTGCTAAAGTGGAATTTTACTACCCCACGGGAT GAGTACATTGAACAGCTGAAGACTCAGATGTCTACCTGTGTGGCTAAATGGTTACAGGATGAAATGTTTC ACTCAGATTTCCAGCATCACAATAAAGCCCTAGCTGTCATGGTTGATCACTTGGAAAGTGAAAAAGACGG TGTCATCAGTTGCTTAGATCTTATCTTAAAATGGCTTACCCTCCGGTTTTTCGACACCAATACAAGCGTT CTAATGAAAGCACTGGAATATTTAAAATTGCTTTTCACCCTGCTGAGTGAGGAAGAGTATCATCTTACTG AGAATGAAGCATCGTCCTTTATCCCCTATCTCATCCTCAAGGTTGGAGAACCAAAGGATGTCATTCGTAA AGATGTCCGTGCTATCCTGAACCGGATGTGCCTTGTCTACCCAGCCAGCAAAATGTTCCCCTTCATTATG GAAGGAACCAAGTCCAAAAACTCTAAGCAGAGAGCAGAGTGCCTGGAAGAGCTAGGTTGCTTAATTGAGT CCTATGGTATGAATGTTTGTCAGCCAACCCCAGGAAAAGCCTTAAAGGAGATAGCTATTCACATAGGAGA CCGAGACAATGCTGTACGGAATGCTGCCCTCAACACCATTGTAACAGTGTACAACGTGCATGGAGATCAG GTGTTCAAACTGATTGGAAATCTTTCTGAGAAAGACATGAGCATGCTTGAGGAGAGGATAAAGCGGTCAG CAAAGAGACCCTCTGCTGCACCTGTGAAACAGGCCGAAGAGAAACCTCAGCGCACACAGAATATAAACTC TAATGCCAACATGCTACGCAAGGGACCAGCTGAGGATATGTCCTCCAAACTTAATCAAGCTCGAAGCCTG AGTGGGCATCCTGAAGCAGCCCAGATGGTTCGCCGAGAATTCCAGCTGGATTTAGATGAGATTGAAAATG ATAATGGTACAGTCCGCTGTGAAATGCCAGAGCTTGTCCAGCACAAACTTGATGACATTTTTGAACCTGT CCTTATTCCTGAACCTAAGATCCGGGCTGTTTCTCCACATTTTGATGACATGCACAGTAATACAGCATCC ACAATCAATTTCATTATCTCCCAAGTAGCCAGTGGTGACATCAACACAAGCATCCAAGCTCTGACACAGA TTGATGAGGTCCTGAGGCAGGAAGACAAAGCTGAAGCTATGTCTGGCCACATTGATCAGTTTCTGATAGC AACATTCATGCAGCTAAGACTCATCTACAGCACACACATGGCAGATGAGAAGTTGGACAAGGATGAAATC ATCAAGTTGTATAGCTGTATCATTGGCAACATGATTTCGCTATTTCAGATAGAGAGCTTGGCCCGGGAGG CCTCCACTGGAGTACTGAAAGACCTGATGCATGGACTGATCACCTTAATGCTGGATTCTCGGATTGAAGA TCTGGAGGAAGGACAACAGGTGATTCGTTCTGTGAACCTCTTGGTGGTGAAGGTTTTGGAGAAATCAGAC CAGACCAATATCCTCAGTGCCCTGCTTGTTTTACTTCAGGACAGCCTGCTAGCAACAGCTAGTTCTCCCA AATTCTCAGAGCTTGTTATGAAGTGTCTATGGAGAATGGTTCGACTATTACCTGACACCATCAACAGCAT TAACCTAGACAGAATTCTTCTGGACATCCACATTTTCATGAAGGTCTTTCCCAAAGAGAAATTGAAGCAA TGCAAAAGTGAATTTCCTATAAGGACTCTAAAGACCCTTCTACATACTTTATGCAAATTAAAAGGACCTA AAATCCTAGACCACCTTACAATGATTGATAATAAGAATGAGTCAGAATTGGAGGCCCACCTGTGTCGGAT GATGAAGCATAGTATGGACCAGACTGGGAGCAAGTCTGATAAGGAAACAGAAAAGGGAGCTTCTCGAATA GATGAAAAATCATCAAAGGCCAAAGTAAATGATTTCTTAGCTGAGATTTTTAAGAAGATTGGCTCCAAAG AGAACACTAAGGAGGGCCTAGCAGAGTTATATGAATATAAGAAGAAATATTCAGATACTGACATTGAACC ATTTCTGAAAAATTCCTCGCAGTTCTTTCAGAGTTATGTTGAGAGAGGCCTTCGGGTGATTGAGATGGAG AGAGAAAGCAAAGGCCGGATTCCTACTTCAACAGGCATTTCCCCTCAGATGGAGGTCACATGTGTGCCCA CCCCCACAAGCACAGTGTCCTCCCTAGGTAACACAAATGGAGAAGAAGTGGGGCCATCTGTCTACTTGGA AAGGCTAAAAATTCTGCGGCAGCGGTGTGGTCTTGACAACACAAAGCAAGATGACCGGCCTCCACTGACC TCTTTGCTCTCCAAACCAGCAATTCCTCCAGTTGCCTCCTCCACAGACATGCTCCATAGCAAGCTCTCAC AGCTCCGGGAGTCCCGGGAACAGCACCAGCACTCAGACCTGGATTCCAACCAGACCCACTCTGCAGGAAC CATGACCTCCTCCTCCTCCACAACTAACATTGATGACTTGAAAAAGAGACTGGAGAGAATAAAGAGCAGT CGCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR217121 protein sequence Red=Cloning site Green=Tags(s) MGDDSEWLKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSPEWSKYLGLIKKFVTDSNAVVQLKGLE AALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKELGIEICLMYVEIEKGESVQEELLKGLDNKNPKI IVACIETLRKALSEFGSKIISLKPIIKVLPKLFESRDKAVRDEAKLFAIEIYRWNRDAVKHTLQNINSVQ LKELEEEWVKLPTGAPKPSRFLRSQQELEAKLEQQQSAGGDAEGGGDDGDEVPQVDAYELLDAVEILSKL PKDFYDKIEAKKWQERKEALEAVEVLVKNPKLEAGDYADLVKALKKVVGKDTNVMLVALAAKCLTGLAVG LRKKFGQYAGHVVPTILEKFKEKKPQVVQALQEAIDAIFLTTTLQNISEDVLAVMDNKNPTIKQQTSLFI ARSFRHCTSSTLPKSLLKPFCAALLKHINDSAPEVRDAAFEALGTALKVVGEKSVNPFLADVDKLKLDRI KECSEKVELVHGKKSGLATEKKESKPLPGRAAASGAAGDKDTKDVSGPKPGPLKKTPTAKAGGPSKKGKT TAPGGSASAGTKNKKGLETKEIVEPELSIEVCEEKASAVLPPTCIQLLDSSNWKERLACMEEFQKAVELM ERTEMPCQALVKMLAKKPGWKETNFQVMQMKLHIVALIAQKGNFSKTSAQIVLDGLVDKIGDVKCGNNAK EAMTAIAEACMLPWTAEQVMSMAFSQKNPKNQSETLNWLSNAIKEFGFSELNVKAFISNVKTALAATNPA VRTSAITLLGVMYLYVGPSLRMIFEDEKPALLSQIDAEFQKMQGQSPPAPTRGIAKHSTSATDEGEDGEE PGEGGNDVVDLLPRIEISDKITSELVSKIGDKNWKIRKEGLDEVAGIINEAKFIQPNIGELPTALKGRLN DSNKILVQQTLNILQQLAVAMGANIRQHVKNLGIPVITVLGDSKNNVRAAALATVNAWAEQTGMKEWLEG EDLSEELKKENPFLRQELLGWLAEKLPTLRSTPTDLILCVPHLYSCLEDRNGDVRKKAQDALPFFMMHLG YEKMAKATGKLKPTSKDQVLAMLEKAKANMPSKPAAPAKAMSKPMGGSAPAKTQPIPAPVEDSVSSTIEA KPDLKKAKAPGVSSKAKSVQGKKVPSKTTLKEDDDKSGPIFIVVPNGKEQRMRDEKGLKVLKWNFTTPRD EYIEQLKTQMSTCVAKWLQDEMFHSDFQHHNKALAVMVDHLESEKDGVISCLDLILKWLTLRFFDTNTSV LMKALEYLKLLFTLLSEEEYHLTENEASSFIPYLILKVGEPKDVIRKDVRAILNRMCLVYPASKMFPFIM EGTKSKNSKQRAECLEELGCLIESYGMNVCQPTPGKALKEIAIHIGDRDNAVRNAALNTIVTVYNVHGDQ VFKLIGNLSEKDMSMLEERIKRSAKRPSAAPVKQAEEKPQRTQNINSNANMLRKGPAEDMSSKLNQARSL SGHPEAAQMVRREFQLDLDEIENDNGTVRCEMPELVQHKLDDIFEPVLIPEPKIRAVSPHFDDMHSNTAS TINFIISQVASGDINTSIQALTQIDEVLRQEDKAEAMSGHIDQFLIATFMQLRLIYSTHMADEKLDKDEI IKLYSCIIGNMISLFQIESLAREASTGVLKDLMHGLITLMLDSRIEDLEEGQQVIRSVNLLVVKVLEKSD QTNILSALLVLLQDSLLATASSPKFSELVMKCLWRMVRLLPDTINSINLDRILLDIHIFMKVFPKEKLKQ CKSEFPIRTLKTLLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKHSMDQTGSKSDKETEKGASRI DEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKYSDTDIEPFLKNSSQFFQSYVERGLRVIEME RESKGRIPTSTGISPQMEVTCVPTPTSTVSSLGNTNGEEVGPSVYLERLKILRQRCGLDNTKQDDRPPLT SLLSKPAIPPVASSTDMLHSKLSQLRESREQHQHSDLDSNQTHSAGTMTSSSSTTNIDDLKKRLERIKSS RK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001165989
ORF Size	6096 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001165989.2
RefSeq Size	6666 bp
RefSeq ORF	6099 bp
Locus ID	75786
MW	225.6 kDa
Gene Summary	Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. In spindle formation protects kinetochore microtubules from depolymerization by KIF2C and has an essential role in centrosomal microtubule assembly independently of KIF2C activity. Contributes to centrosome integrity. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments.[UniProtKB/Swiss-Prot Function]

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