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Ppp1r15b (NM_133819) Mouse Tagged ORF Clone
CAT＃: MR217037
- TrueORF®
Ppp1r15b (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 15b (Ppp1r15b)
ORF Plasmid: tGFP
"NM_133819" in other vectors (4)
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CNY 5,104.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1810033K10Rik; AI606441; C530022L24Rik; CReP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217037 representing NM_133819
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGAGACAGGAACGCACAGGGCCCGGAAGCGGCCTGGCCCTCGGCTGGGCTCCTGGTTCCGGCTGCCCT TCCTTCGGCGATCGCACGCCTGCTCTTCGGAGTTCCCGCCGCCTTCCTCTCGACAAAATCCCGGGAACTC CGCTCTGCCCGAGCGTCGGACCAGGTACTGGACCAAATTGCTTTCTCAGCTCCTTGCCCTGCTCCCTAGC CTATTCCAGAAGCTGCTGCTTTGGAGCCAGCTTTTCGGGGGCCTGATTCCTACCAGATGGCTAGATTTTG CCGCAAGTTACAGCGCCCTGAGAGCTTTGAGAGGACGGGAGGAATCTGCCGCTCCCACGGTGCAGAAGTC TCTGAGTTCACTGCGGCTGGACTCTTCGGAAGACTTGGTTGTCAGTTCTCTTGATTGGCTAGAGGAGGGA CTCCAGTGGCAGTGCTCGTCCTCAGACCTGGAGTTAAAACTCAAGGCCCAGGAAAGAGCTTTAGACTCTG CAGCGCCCACTTTCCTCCTGGAGCAGCAGCTGTGGGGAGTGGAGTTGCTGCCCAGTAGCCTTCAAGCTGG TCTAGTCTCCCACCGAGAACTTGACTCTTCATCCTCTGGGCCTCTGAGCGTTCAGAGCTTAGGTAATTTC AAGGTAGTTTCCTATCTCCTGAACCCTTCCTACCTGGACTACCTTCCCCAGTTAGGGCTGCGCTGTCAGA GCAGCGCTGGAGGTGGCCAGTTTGTGGGTTTCCGAACACTAACCCCAGAGAGCTGCTATCTTTCTGAAGA TGGTTGTCACCCTCAGCCGTTGCGGGCAGAGATGTCGGCAACCGCCTGGAGAAGGTGTCCGCCTCTCTCT ACAGAAGGCCTGCCGGAAATCCACCACCTTCGTATGAAACGGCTAGAATTCCTCCAGGCTAACAAAGGGC AAGAGTTACCCACCCCTGACCAAGATAATGGCTATCATAGCCTGGAGGAGGAACATAACCTTCTCCGGAT GGACCCACAACATTGCACAGATAACCCAGCACAGGCGGTGTCCCCTGCTGCAGACAGGCCGGAGCCCACT GAGAAAAAACCAGAATTGGTGATTCAAGAAGTTTCACAGAGCCCCCAGGGAAGCAGTCTGTTTTGTGAAT TACCCGTGGAAAAAGAATGTGAAGAGGACCACACTAATGCAACTGACCTCTCAGATAGAGGAGAGAGCCT TCCTGTTTCTACCAGACCAGTTTGTAGCAACAAACTGATAGATTATATTTTGGGAGGCGCCCCCAGTGAC TTGGAAGCCAGCTCTGATTCTGAAAGTGAGGATTGGGGCGAGGAACCTGAGGACGATGGCTTTGATAGCG ATGGCTCCCTGTCTGAATCAGACGTGGAACAGGACTCGGAAGGCCTTCACCTTTGGAACTCTTTCCACAG TGTAGATCCTTACAACCCCCAAAACTTTACAGCCACGATTCAGACGGCTGCCAGAATTGCCCCCAGAGAC CCATCAGATTCAGGGACATCCTGGTCTGGCAGCTGTGGTGTAGGGAGCTGTCAGGAGGGACCCCTTCCGG AGACCCCCGACCATAGTTCCGGGGAGGAAGATGACTGGGAACCGAGTGCAGATGAAGCAGAGAATCTTAA ATTGTGGAACTCTTTCTGTCATTCTGAGGACCCCTACAACCTTTTAAATTTTAAGGCTCCTTTTCAACCG TCAGGGAAGAATTGGAAAGGCCGTCAGGACTCAAAGGCCTCTTCTGAGGCTACAGTGGCCTTCTCTGGCC ATCATACCTTACTTTCTTGTAAGGCCCAGCTGTTAGAGAGCCAAGAAGATAATTGTCCAGGCTGTGGGCT GGGTGAGGCTCTTGCTGGAGAAAGATACACCCATATCAAGAGAAAAAAGGTAACCTTCCTGGAAGAAGTT ACTGAGTATTATATAAGTGGTGATGAGGATCGCAAAGGACCATGGGAAGAATTTGCAAGGGATGGATGCA GGTTCCAGAAACGAATTCAAGAAACAGAAGTTGCCATTGGCTACTGCTTGGCCTTTGAGCACAGAGAAAA AATGTTTAATAGACTGAGGATCGAGTCAAAGGACTTACTGTTGTACAGCAATGTTAAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217037 representing NM_133819
Red=Cloning site Green=Tags(s) METGTHRARKRPGPRLGSWFRLPFLRRSHACSSEFPPPSSRQNPGNSALPERRTRYWTKLLSQLLALLPS LFQKLLLWSQLFGGLIPTRWLDFAASYSALRALRGREESAAPTVQKSLSSLRLDSSEDLVVSSLDWLEEG LQWQCSSSDLELKLKAQERALDSAAPTFLLEQQLWGVELLPSSLQAGLVSHRELDSSSSGPLSVQSLGNF KVVSYLLNPSYLDYLPQLGLRCQSSAGGGQFVGFRTLTPESCYLSEDGCHPQPLRAEMSATAWRRCPPLS TEGLPEIHHLRMKRLEFLQANKGQELPTPDQDNGYHSLEEEHNLLRMDPQHCTDNPAQAVSPAADRPEPT EKKPELVIQEVSQSPQGSSLFCELPVEKECEEDHTNATDLSDRGESLPVSTRPVCSNKLIDYILGGAPSD LEASSDSESEDWGEEPEDDGFDSDGSLSESDVEQDSEGLHLWNSFHSVDPYNPQNFTATIQTAARIAPRD PSDSGTSWSGSCGVGSCQEGPLPETPDHSSGEEDDWEPSADEAENLKLWNSFCHSEDPYNLLNFKAPFQP SGKNWKGRQDSKASSEATVAFSGHHTLLSCKAQLLESQEDNCPGCGLGEALAGERYTHIKRKKVTFLEEV TEYYISGDEDRKGPWEEFARDGCRFQKRIQETEVAIGYCLAFEHREKMFNRLRIESKDLLLYSNVKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133819 |
| ORF Size | 2091 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133819.3, NP_598580.1 |
| RefSeq Size | 5588 bp |
| RefSeq ORF | 2094 bp |
| Locus ID | 108954 |
| UniProt ID | Q8BFW3 |
| MW | 77.7 kDa |
| Gene Summary | Maintains low levels of EIF2S1 phosphorylation in unstressed cells by promoting its dephosphorylation by PP1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220606 | Ppp1r15b (untagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 15b (Ppp1r15b), (10ug) |
CNY 9,030.00 |
|
| MG217037 | Ppp1r15b (tGFP-tagged) - Mouse protein phosphatase 1 regulatory (inhibitor) subunit 15b (Ppp1r15b), (10ug) |
CNY 6,370.00 |
|
| MR217037L3 | Lenti ORF clone of Ppp1r15b (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 15b (Ppp1r15b) |
CNY 7,700.00 |
|
| MR217037L4 | Lenti ORF clone of Ppp1r15b (mGFP-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 15b (Ppp1r15b) |
CNY 7,700.00 |
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