Alg10b (NM_001033441) Mouse Tagged ORF Clone
CAT#: MR216801
- TrueORF®
Alg10b (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 10 homolog B (yeast, alpha-1,2-glucosyltransferase) (Alg10b)
ORF Plasmid: tGFP
"NM_001033441" in other vectors (4)
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CNY 3,656.00
CNY 4,180.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AA469671; AU045404; AW123895; C85569; Deaf1; Gm917; nse5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216801 representing NM_001033441
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGCTGGAGGGCTATTACTTCTCGGCCGCCTTGAGCTGCACCTTCCTGGTGTCCTGCCTGCTCT TCTCCGCCTTCAGCCGCGCTCTGCGGGAGCCGTACATGGACGAGATCTTCCACCTGCCGCAGGCGCAGCG GTACTGCGAGGGCCGCTTCTCCCTGTCGCAGTGGGATCCTATGATCACTACATTACCTGGCCTGTATCTG GTGTCGGTCGGTGTGGTCAAACCTGCCAGCTGGCTCCTTGGATGGTCTGAGCATGTGATCTGCTCCATTG GGGTGCTCAGATTTGTCAATCTTCTCTTCAGCGTTGGCAACTTCTACTTGCTGTATTTGCTCTTCAGGAA GGTGCAACCCAGAAACAAGGCTTCTTCAAGTATCCAGAGAATCTTGTCAACATTAACCCTAGCAGTGTTT CCAACCCTCTATTTTTTTAACTTCCTTTACTATACAGAAGCTGGGTCTGTATTCTTCACTCTTTTTGCTT ATTTGATGTGTCTTTATGGCAACCATAGGACCTCGGCCTTGCTTGGATTTTGTGGTTTCATGTTTCGTCA GACCAACATCATCTGGGCGGCCTTCTGTGCAGGACACCTCATTGCACAGAAGTGCAGTGAAGCCTGGAAG ATTGAGCTGCAGAAGAAGAAGGAGGAGAGGCTTGCGCCCACGAAGGGACCGCTCTCGGAGCTCAGAAGAG TGCTGCAGTTTCTGCTGGTGTACGCCATGTCCCTAAAGAACCTGAGAATGCTCTTCCTGTTGACCTGGCC CTACGTGCTTCTGCTGTTGGCGTTCTTTGCCTTTGTGGTAGTTAATGGTGGCATTGTTGTTGGTGACCGG AGTAGTCACGAGGCCTGTCTCCATTTTCCTCAGTTGTTCTATTTCTTCTCCTTTACTGCCTTTTTCTCCT TCCCTCACCTACTTTCTCTGACCAAAGTCAAGACTTTCCTTAGCTTAGTTTGGAAGCGTAGAGTCCAGTT CTCTGTGGTTACGTTAGTCTCGATACTTTTGGTTTGGAAATTCACTTATGTCCATAAGTATTTACTGGCA GACAATAGGCATTACACATTTTATGTGTGGAAAAGAGTATTTCAGAGACATGAAGTTGTCAAATATTTAT TAGTTCCAGCCTACATTTTTGCTGGTTGGGCTATAGCTGACTCTTTAAAGGCTAAGTCAATTTTCTGGAA TTTAATGTTTTTTGTATGCTTGGTTGCTTCTACAGTTCCTCAGAAACTACTAGAATTCCGTTACTTCATT CTACCATACATTATTTATAGGCTTAACATACCTCTGCCACCCATATCTAGACTCGTTTGTGAACTGGGTT GCTATACAGTTGTTAATTTTGTAACTTTTTATATCTTTCTGAACAAGACTTTTCAGTGGCCAAATAGTCA GGACATCCAAAGGTTTATGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216801 representing NM_001033441
Red=Cloning site Green=Tags(s) MAQLEGYYFSAALSCTFLVSCLLFSAFSRALREPYMDEIFHLPQAQRYCEGRFSLSQWDPMITTLPGLYL VSVGVVKPASWLLGWSEHVICSIGVLRFVNLLFSVGNFYLLYLLFRKVQPRNKASSSIQRILSTLTLAVF PTLYFFNFLYYTEAGSVFFTLFAYLMCLYGNHRTSALLGFCGFMFRQTNIIWAAFCAGHLIAQKCSEAWK IELQKKKEERLAPTKGPLSELRRVLQFLLVYAMSLKNLRMLFLLTWPYVLLLLAFFAFVVVNGGIVVGDR SSHEACLHFPQLFYFFSFTAFFSFPHLLSLTKVKTFLSLVWKRRVQFSVVTLVSILLVWKFTYVHKYLLA DNRHYTFYVWKRVFQRHEVVKYLLVPAYIFAGWAIADSLKAKSIFWNLMFFVCLVASTVPQKLLEFRYFI LPYIIYRLNIPLPPISRLVCELGCYTVVNFVTFYIFLNKTFQWPNSQDIQRFMW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001033441 |
ORF Size | 1422 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001033441.3, NP_001028613.1 |
RefSeq Size | 3727 bp |
RefSeq ORF | 1425 bp |
Locus ID | 380959 |
UniProt ID | Q3UGP8 |
MW | 55.9 kDa |
Gene Summary | Putative alpha-1,2-glucosyltransferase, which adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(2)Man(9)GlcNAc(2)-PP-Dol (By similarity). When coupled to KCNH2 may reduce KCNH2 sensitivity to classic proarrhythmic drug blockade, possibly by mediating glycosylation of KCNH2 (By similarity). Has a role in maintenance of cochlear outer hair cell function (PubMed:24303013).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216576 | Alg10b (untagged) - Mouse asparagine-linked glycosylation 10 homolog B (yeast, alpha-1,2-glucosyltransferase) (Alg10b), (10ug) |
CNY 6,180.00 |
|
MG216801 | Alg10b (tGFP-tagged) - Mouse asparagine-linked glycosylation 10 homolog B (yeast alpha-12-glucosyltransferase) (Alg10b), (10ug) |
CNY 4,560.00 |
|
MR216801L3 | Lenti ORF clone of Alg10b (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 10 homolog B (yeast, alpha-1,2-glucosyltransferase) (Alg10b) |
CNY 6,080.00 |
|
MR216801L4 | Lenti ORF clone of Alg10b (mGFP-tagged) - Mouse asparagine-linked glycosylation 10 homolog B (yeast, alpha-1,2-glucosyltransferase) (Alg10b) |
CNY 6,080.00 |