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Kcnc2 (NM_001025581) Mouse Tagged ORF Clone
CAT＃: MR216588
- TrueORF®
Kcnc2 (Myc-DDK-tagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 2 (Kcnc2)
ORF Plasmid: tGFP
"NM_001025581" in other vectors (4)
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CNY 4,760.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW047325; B230117I07; KShIIIA; Kv3.2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216588 representing NM_001025581
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAAGATCGAGAGCAACGAGAGGGTGATCCTCAATGTCGGGGGTACCAGGCACGAAACCTACCGCA GCACCCTCAAGACCCTGCCTGGAACTCGCCTGGCCCTTCTTGCCTCCTCTGAACCTCAGGGCGACTGCCT GACTGCGGCCGGGGACAAGCTGCAACCGCTGCCCCCTCCGCTGTCTCCGCCGCCACGACCGCCTCCCTTG TCCCCTGTCCCCAGCGGCTGCTTCGAGGGCGGCGCAGGCAACTGCAGTTCGCACGGTGGCAACGGCGGCA ACGGCGGCAGCGACCACCCTGGGGGAGGCCGCGAATTCTTCTTCGATCGCCACCCAGGAGTATTCGCCTA TGTGCTCAATTACTACCGCACGGGCAAGCTGCACTGCCCCGCCGACGTGTGCGGGCCGCTCTTCGAGGAA GAGCTGGCTTTCTGGGGCATCGATGAGACCGACGTGGAGCCCTGCTGCTGGATGACCTACAGGCAGCACC GGGACGCGGAGGAGGCCCTGGACATCTTTGAGACACCCGACCTCATCGGGGGCGACCCTGGTGATGATGA GGACCTAGCGGCCAAGAGATTGGGCATTGAGGATGCTGCGGGGCTGGGAGGACCCGATGGCAAGTCAGGC CGCTGGAGGAAGCTGCAGCCTCGCATGTGGGCTCTTTTTGAGGACCCCTACTCATCTAGAGCCGCTAGGT TTATTGCTTTTGCTTCTTTGTTCTTCATTTTGGTTTCCATCACAACCTTTTGCCTGGAGACACATGAAGC TTTCAATATTGTTAAAAACAAGACGGAGCCCGTCATCAATGGCACCAGCCCGGTCCTCCAGTACGAAATC GAAACGGATCCCGCCCTGACGTACGTGGAAGGAGTATGTGTGGTGTGGTTTACGTTTGAATTTTTAGTCC GAATTGTTTTCTCACCCAATAAACTTGAATTCATCAAAAATCTCTTGAACATCATTGACTTTGTGGCCAT CCTCCCCTTCTACCTAGAGGTGGGACTCAGCGGGCTGTCCTCCAAAGCGGCCAAAGATGTGCTCGGCTTT CTCAGGGTGGTTAGGTTTGTGAGGATCCTGAGAATCTTCAAGCTCACCCGCCATTTCGTAGGTCTGAGGG TGCTCGGACATACTCTTCGGGCGAGCACCAACGAATTTTTGTTGCTGATCATCTTCCTGGCGCTGGGAGT TTTGATATTCGCTACGATGATCTACTACGCTGAGAGAGTAGGGGCTCAGCCCAATGACCCTTCAGCTAGT GAGCACACGCAGTTCAAAAACATCCCCATTGGTTTCTGGTGGGCCGTAGTGACCATGACTACCTTAGGTT ACGGGGATATGTACCCCCAAACATGGTCAGGGATGTTGGTGGGGGCCTTGTGTGCCCTGGCCGGAGTGCT GACAATAGCCATGCCTGTGCCTGTCATTGTCAACAATTTTGGAATGTACTACTCCTTGGCAATGGCGAAG CAGAAACTTCCAAGAAAGAGAAAGAAGCATATTCCTCCTGCCCCTCTGGCAAGCTCGCCTACATTTTGCA AGACAGAATTGAACATGGCTTGCAACAGTACCCAGAGTGACACATGTCTGGGCAAAGAAAACCGGCTTCT GGAACATAACAGATCAGTGTTATCAGGTGACGACAGTACAGGAAGTGAGCCGCCATTATCACCTCCAGAA AGGCTCCCTATCAGACGCTCTAGTACCAGAGACAAAAACAGAAGAGGGGAAACGTGTTTCCTGTTGACGA CAGGTGATTACACGTGCGCTTCTGATGGAGGAATCAGGAAAGCAAGCACACTAGAGCCCATGGAGAGTAC TGCACAGACTAAAGGAGACACAAGACCAGAAGCTCATTGGAATTGTGCGCACTTACTCAATTTTGGGTGT CCTACAGGAAGTTCATTTCCCACCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216588 representing NM_001025581
Red=Cloning site Green=Tags(s) MGKIESNERVILNVGGTRHETYRSTLKTLPGTRLALLASSEPQGDCLTAAGDKLQPLPPPLSPPPRPPPL SPVPSGCFEGGAGNCSSHGGNGGNGGSDHPGGGREFFFDRHPGVFAYVLNYYRTGKLHCPADVCGPLFEE ELAFWGIDETDVEPCCWMTYRQHRDAEEALDIFETPDLIGGDPGDDEDLAAKRLGIEDAAGLGGPDGKSG RWRKLQPRMWALFEDPYSSRAARFIAFASLFFILVSITTFCLETHEAFNIVKNKTEPVINGTSPVLQYEI ETDPALTYVEGVCVVWFTFEFLVRIVFSPNKLEFIKNLLNIIDFVAILPFYLEVGLSGLSSKAAKDVLGF LRVVRFVRILRIFKLTRHFVGLRVLGHTLRASTNEFLLLIIFLALGVLIFATMIYYAERVGAQPNDPSAS EHTQFKNIPIGFWWAVVTMTTLGYGDMYPQTWSGMLVGALCALAGVLTIAMPVPVIVNNFGMYYSLAMAK QKLPRKRKKHIPPAPLASSPTFCKTELNMACNSTQSDTCLGKENRLLEHNRSVLSGDDSTGSEPPLSPPE RLPIRRSSTRDKNRRGETCFLLTTGDYTCASDGGIRKASTLEPMESTAQTKGDTRPEAHWNCAHLLNFGC PTGSSFPTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025581 |
| ORF Size | 1917 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001025581.1, NP_001020752.1 |
| RefSeq Size | 6196 bp |
| RefSeq ORF | 1920 bp |
| Locus ID | 268345 |
| MW | 70.6 kDa |
| Gene Summary | Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain. Contributes to the regulation of the fast action potential repolarization and in sustained high-frequency firing in neurons of the central nervous system (PubMed:10561420, PubMed:10414303, PubMed:11124984, PubMed:10903572, PubMed:11506885, PubMed:15317859, PubMed:15917463, PubMed:17761775, PubMed:21414897). Homotetramer channels mediate delayed-rectifier voltage-dependent potassium currents that activate rapidly at high-threshold voltages and inactivate slowly (PubMed:10414303). Forms tetrameric channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (By similarity). Can form functional homotetrameric and heterotetrameric channels that contain variable proportions of KCNC1, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (PubMed:10531438, PubMed:12000114). Channel properties may be modulated by either the association with ancillary subunits, such as KCNE1, KCNE2 and KCNE3 or indirectly by nitric oxide (NO) through a cGMP- and PKG-mediated signaling cascade, slowing channel activation and deactivation of delayed rectifier potassium channels (By similarity). Contributes to fire sustained trains of very brief action potentials at high frequency in thalamocortical and suprachiasmatic nucleus (SCN) neurons, in hippocampal and neocortical interneurons and in retinal ganglion cells (PubMed:10561420, PubMed:10903572, PubMed:11506885, PubMed:17761775). Sustained maximal action potential firing frequency in inhibitory hippocampal interneurons is negatively modulated by histamine H2 receptor activation in a cAMP- and protein kinase (PKA) phosphorylation-dependent manner (PubMed:10903572). Plays a role in maintaining the fidelity of synaptic transmission in neocortical GABAergic interneurons by generating action potential (AP) repolarization at nerve terminals, thus reducing spike-evoked calcium influx and GABA neurotransmitter release (PubMed:15917463). Required for long-range synchronization of gamma oscillations over distance in the neocortex (PubMed:22539821). Contributes to the modulation of the circadian rhythm of spontaneous action potential firing in suprachiasmatic nucleus (SCN) neurons in a light-dependent manner (PubMed:21414897).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
An integrative study identifies KCNC2 as a novel predisposing factor for childhood obesity and the risk of diabetes in the Korean population
,Hwang, JY;Lee, HJ;Go, MJ;Jang, HB;Park, SI;Kim, BJ;Lee, HJ;,
Sci Rep
,PubMed ID 27623749
[Kcnc2]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220043 | Kcnc2 (untagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 2 (Kcnc2), (10ug) |
CNY 8,270.00 |
|
| MG216588 | Kcnc2 (tGFP-tagged) - Mouse potassium voltage gated channel Shaw-related subfamily member 2 (Kcnc2), (10ug) |
CNY 5,040.00 |
|
| MR216588L3 | Lenti ORF clone of Kcnc2 (Myc-DDK-tagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 2 (Kcnc2) |
CNY 6,460.00 |
|
| MR216588L4 | Lenti ORF clone of Kcnc2 (mGFP-tagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 2 (Kcnc2) |
CNY 6,460.00 |
