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Racgap1 (NM_012025) Mouse Tagged Lenti ORF Clone
CAT＃: MR216578L3
- LentiORF®
Lenti ORF clone of Racgap1 (Myc-DDK-tagged) - Mouse Rac GTPase-activating protein 1 (Racgap1)
"NM_012025" in other vectors (3)
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CNY 8,170.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI227039; AI327394; Band25; gtl11; GTPase; MgcRacGAP; mKIAA1478 |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR216578).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012025 |
| ORF Size | 1884 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012025.7, NP_036155.1 |
| RefSeq Size | 3071 bp |
| RefSeq ORF | 1887 bp |
| Locus ID | 26934 |
| UniProt ID | Q9WVM1 |
| Gene Summary | Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG216578 | Racgap1 (tGFP-tagged) - Mouse Rac GTPase-activating protein 1 (Racgap1), (10ug) |
CNY 6,940.00 |
|
| MR216578 | Racgap1 (Myc-DDK-tagged) - Mouse Rac GTPase-activating protein 1 (Racgap1) |
CNY 6,270.00 |
|
| MR216578L4 | Lenti ORF clone of Racgap1 (mGFP-tagged) - Mouse Rac GTPase-activating protein 1 (Racgap1) |
CNY 8,170.00 |
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