Syt3 (NM_001114116) Mouse Tagged ORF Clone
CAT#: MR216575
- TrueORF®
Syt3 (Myc-DDK-tagged) - Mouse synaptotagmin III (Syt3), transcript variant 2
ORF Plasmid: tGFP
"NM_001114116" in other vectors (3)
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CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI385753; sytIII |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216575 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGGGACTATGAAGATGACCTCTGTCGGCGGGCGCTCATCCTGGTCTCAGACCTCTGTGCACGGG TCCGAGATGCTGATACCAATGATAGGTGTCAAGAGTTCAACGAACTGCGGATCAGAGGATATCCCCGGGG TCCGGATGCAGATATTTCCGTGAGCCTGCTGTCAGTCATCGTGACATTCTGTGGCATCGTCCTCTTGGGT GTCTCCCTCTTCGTGTCCTGGAAGTTGTGCTGGGTGCCCTGGAGGGACAAAGGAGGCTCAGCAGTGGGTG GTGGCCCCCTGCGCAAAGACTTAGCCCCTGGGGTTGGGCTGGCAGGCCTGGTAGGAGGTGGTGGGCACCA CCTAGGAGCCAGCCTCGGTGGTCATCCCCTGCTGGGTGGCCCACACCATCATGGGCACACAGCCCACCAT CCACCATTTGCGGAGCTGCTGGAACCAGGTGGTCTTGGGGGCTCTGAGCCGCCAGAACCTTCCTACCTGG ACATGGACTCATATCCAGAGGCTGCTGTGGCCTCTGTGGTAGCTGCTGGAGTCAAACCAAGCCAGACATC CCCTGAGCTGCCTTCTGAGGGTGGAACAGGTTCTGGGCTGCTCCTGCTGCCTCCCAGTGGTGGGGGCTTA CCCAGTGCCCAGTCTCATCAGCAGGTCACAAGTCTAGCACCCACCACAAGGTACCCAGCCCTGCCTCGGC CCCTCACCCAGCAGACCCTGACCACCCAAGCAGACCCAAGCACTGAGGAGCGACCGCCTGCACTGCCCTT ACCTCTGCCGGGTGGAGAGGAAAAAGCCAAGCTCATCGGTCAGATCAAACCAGAGCTGTACCAGGGCACT GGACCCGGTGGTCGGCGAGGCGGGGGCTCAGGGGAAGCAGGGGCACCCTGTGGCCGCATCAGTTTTGCCC TCCGGTACCTCTATGGTTCTGACCAGCTCGTGGTGCGGATCCTACAGGCCCTGGATCTCCCAGCAAAGGA CTCCAATGGGTTCTCAGACCCCTACGTCAAGATCTACCTGTTGCCTGATCGCAAAAAGAAGTTCCAGACC AAGGTGCACAGGAAGACGTTGAACCCCATCTTCAACGAGACGTTTCAGTTCTCGGTGCCCCTGGCCGAGC TGGCACAGCGCAAGCTGCACTTCAGCGTCTATGACTTCGACCGCTTCTCGCGGCATGATCTCATTGGCCA GGTGGTTTTGGACAACCTGCTGGAGCTGGCTGAACAGCCCCCTGACCGCCCGCTCTGGAGGGACATCCTG GAGGGTGGCTCGGAAAAGGCAGATCTCGGAGAGCTCAACTTCTCTCTCTGCTACCTCCCCACGGCTGGGC GCCTCACCGTGACCATCATCAAAGCCTCTAACCTCAAAGCAATGGACCTCACTGGTTTCTCAGACCCCTA TGTGAAGGCCTCTCTGATCAGCGAGGGGCGGCGTCTGAAGAAACGGAAGACCTCCATTAAGAAGAACACA CTGAACCCCACGTACAACGAGGCCCTGGTGTTCGACGTGGCTCCCGAGAGCGTGGAGAACGTGGGTCTCA GCATCGCAGTGGTAGACTATGACTGCATCGGCCACAATGAAGTGATCGGGGTGTGCCGCGTGGGTCCAGA GGCTGCCGACCCACACGGCAGAGAACACTGGGCAGAGATGCTGGCTAACCCGCGCAAGCCTGTGGAACAC TGGCACCAGCTAGTGGAGGAGAAGACTCTGAGCAGCTTCACAAAGGGTGGCAAAGGATTGTCAGAGAAAG AGAATTCAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216575 protein sequence
Red=Cloning site Green=Tags(s) MSGDYEDDLCRRALILVSDLCARVRDADTNDRCQEFNELRIRGYPRGPDADISVSLLSVIVTFCGIVLLG VSLFVSWKLCWVPWRDKGGSAVGGGPLRKDLAPGVGLAGLVGGGGHHLGASLGGHPLLGGPHHHGHTAHH PPFAELLEPGGLGGSEPPEPSYLDMDSYPEAAVASVVAAGVKPSQTSPELPSEGGTGSGLLLLPPSGGGL PSAQSHQQVTSLAPTTRYPALPRPLTQQTLTTQADPSTEERPPALPLPLPGGEEKAKLIGQIKPELYQGT GPGGRRGGGSGEAGAPCGRISFALRYLYGSDQLVVRILQALDLPAKDSNGFSDPYVKIYLLPDRKKKFQT KVHRKTLNPIFNETFQFSVPLAELAQRKLHFSVYDFDRFSRHDLIGQVVLDNLLELAEQPPDRPLWRDIL EGGSEKADLGELNFSLCYLPTAGRLTVTIIKASNLKAMDLTGFSDPYVKASLISEGRRLKKRKTSIKKNT LNPTYNEALVFDVAPESVENVGLSIAVVDYDCIGHNEVIGVCRVGPEAADPHGREHWAEMLANPRKPVEH WHQLVEEKTLSSFTKGGKGLSEKENSE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001114116 |
ORF Size | 1761 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001114116.1, NP_001107588.1 |
RefSeq Size | 2497 bp |
RefSeq ORF | 1764 bp |
Locus ID | 20981 |
UniProt ID | O35681 |
MW | 63.2 kDa |
Gene Summary | Ca(2+) sensor involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain. Ca(2+) induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis. Plays a role in dendrite formation by melanocytes.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG216575 | Syt3 (tGFP-tagged) - Mouse synaptotagmin III (Syt3) transcript variant 2, (10ug) |
CNY 5,040.00 |
|
MR216575L3 | Lenti ORF clone of Syt3 (Myc-DDK-tagged) - Mouse synaptotagmin III (Syt3), transcript variant 2 |
CNY 6,460.00 |
|
MR216575L4 | Lenti ORF clone of Syt3 (mGFP-tagged) - Mouse synaptotagmin III (Syt3), transcript variant 2 |
CNY 6,460.00 |