Dpy30 (NM_001146222) Mouse Tagged ORF Clone
CAT#: MR216533
- TrueORF®
Dpy30 (Myc-DDK-tagged) - Mouse dpy-30 homolog (C. elegans) (Dpy30), transcript variant 2
ORF Plasmid: tGFP
"NM_001146222" in other vectors (3)
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CNY 1,200.00
CNY 3,810.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2810410M20Rik; C87842 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216533 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCGGAGCAGATGCTGGAGGGACAGACACAGGTTGCAGAAAACCCTCACTCTGAGTACGGACTCA CAGACAGCGTTGAGAGAATAGTCGAAAATGAGAAGATTAATGCAGAAAAGTCATCGAAACAGAAGGTGGA TCTACAGTCCTTGCCCACCCGTGCCTACCTGGACCAGACAGTTGTGCCTATCTTATTACAGGGACTTGCT GTGCTTGCCAAGGAAAGACCACCAAATCCCATCGAGTTCCTAGCATCTTATCTTTTAAAAAACAAGGCGC AGTTTGAAGATCGAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216533 protein sequence
Red=Cloning site Green=Tags(s) MESEQMLEGQTQVAENPHSEYGLTDSVERIVENEKINAEKSSKQKVDLQSLPTRAYLDQTVVPILLQGLA VLAKERPPNPIEFLASYLLKNKAQFEDRN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001146222 |
ORF Size | 300 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001146222.1, NP_001139694.1 |
RefSeq Size | 790 bp |
RefSeq ORF | 300 bp |
Locus ID | 66310 |
UniProt ID | Q99LT0 |
MW | 11.2 kDa |
Gene Summary | As part of the MLL1/MLL complex, involved in the methylation of histone H3 at 'Lys-4', particularly trimethylation. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. May play some role in histone H3 acetylation. In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and histone H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid. Does not affect ES cell self-renewal. May also play an indirect or direct role in endosomal transport.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG216533 | Dpy30 (tGFP-tagged) - Mouse dpy-30 homolog (C. elegans) (Dpy30) transcript variant 2, (10ug) |
CNY 2,850.00 |
|
MR216533L3 | Lenti ORF clone of Dpy30 (Myc-DDK-tagged) - Mouse dpy-30 homolog (C. elegans) (Dpy30), transcript variant 2 |
CNY 4,750.00 |
|
MR216533L4 | Lenti ORF clone of Dpy30 (mGFP-tagged) - Mouse dpy-30 homolog (C. elegans) (Dpy30), transcript variant 2 |
CNY 4,750.00 |