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Wdr82 (NM_029896) Mouse Tagged ORF Clone
CAT＃: MR216454
- TrueORF®
Wdr82 (Myc-DDK-tagged) - Mouse WD repeat domain containing 82 (Wdr82)
ORF Plasmid: tGFP
"NM_029896" in other vectors (4)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9430077D24Rik; CDW5/WDR82 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216454 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTGACCGACAGCGTGCTCCGGAGCTTCCGCGTCGCCAAGGTGTTCCGCGAGAACTCAGACAAGA TCAACTGCTTTGACTTTAGTCCCAATGGCGAGACGGTCATCTCTAGCAGCGATGATGACTCCATCGTGCT CTATGACTGCCAGGAGGGCAAACCAAAGAGAACCCTGTACAGTAAGAAGTACGGTGTGGACCTCATCAGA TACACCCATGCAGCCAACACAGTCGTTTACAGCTCTAACAAAATAGACGATACTATTCGTTACTTGTCCT TGCATGACAATAAATACATCAGATACTTTCCTGGACACAGCAAGAGGGTGGTGGCCTTGTCCATGTCACC TGTGGATGACACTTTCATTTCTGGGTCTCTTGATAAGACCATTCGACTCTGGGATCTCCGGTCTCCTAAC TGCCAGGGCCTCATGCATCTACAGGGCAAACCTGTCTGTTCCTTTGATCCAGAAGGGTTAATTTTTGCTG CGGGCGTCAACTCAGAAATGGTCAAACTTTATGACCTTCGTTCTTTTGATAAGGGACCATTTGCAACATT TAAGATGCAGTATGATAGGACCTGTGAGTGGACAGGACTTAAGTTCAGCAATGATGGCAAATTGATACTC ATCTCCACCAACGGCAGCTTTATCCGACTGATTGACGCATTCAAGGGTGTGGTGATGCACACATTTGGGG GTTATGCTAACAGCAAAGCTGTGACACTCGAAGCTTCATTTACTCCAGACTCACAGTTTATTATGATCGG TTCAGAAGACGGCAAAATCCACGTCTGGAATGGAGAGAGTGGTATAAAAGTGGCTGTATTGGATGGCAAA CACACTGGTCCCATTACCTGTTTGCAATTCAACCCCAAGTTTATGACCTTTGCCAGCGCCTGTTCCAACA TGGCCTTCTGGTTGCCCACCATTGATGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216454 protein sequence
Red=Cloning site Green=Tags(s) MKLTDSVLRSFRVAKVFRENSDKINCFDFSPNGETVISSSDDDSIVLYDCQEGKPKRTLYSKKYGVDLIR YTHAANTVVYSSNKIDDTIRYLSLHDNKYIRYFPGHSKRVVALSMSPVDDTFISGSLDKTIRLWDLRSPN CQGLMHLQGKPVCSFDPEGLIFAAGVNSEMVKLYDLRSFDKGPFATFKMQYDRTCEWTGLKFSNDGKLIL ISTNGSFIRLIDAFKGVVMHTFGGYANSKAVTLEASFTPDSQFIMIGSEDGKIHVWNGESGIKVAVLDGK HTGPITCLQFNPKFMTFASACSNMAFWLPTIDD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029896 |
| ORF Size | 942 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029896.1, NP_084172.1 |
| RefSeq Size | 4313 bp |
| RefSeq ORF | 942 bp |
| Locus ID | 77305 |
| UniProt ID | Q8BFQ4 |
| MW | 35.1 kDa |
| Gene Summary | Regulatory component of the SET1 complex implicated in the tethering of this complex to transcriptional start sites of active genes. Facilitates histone H3 'Lys-4' methylation via recruitment of the SETD1A or SETD1B to the 'Ser-5' phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Possible role in telomere length maintenance and in mRNA processing (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211815 | Wdr82 (untagged) - Mouse WD repeat domain containing 82 (Wdr82), (10ug) |
CNY 3,990.00 |
|
| MG216454 | Wdr82 (tGFP-tagged) - Mouse WD repeat domain containing 82 (Wdr82), (10ug) |
CNY 2,850.00 |
|
| MR216454L3 | Lenti ORF clone of Wdr82 (Myc-DDK-tagged) - Mouse WD repeat domain containing 82 (Wdr82) |
CNY 4,750.00 |
|
| MR216454L4 | Lenti ORF clone of Wdr82 (mGFP-tagged) - Mouse WD repeat domain containing 82 (Wdr82) |
CNY 4,750.00 |
