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Cyb5r4 (NM_024195) Mouse Tagged ORF Clone
CAT＃: MR216118
- TrueORF®
Cyb5r4 (Myc-DDK-tagged) - Mouse cytochrome b5 reductase 4 (Cyb5r4)
ORF Plasmid: tGFP
"NM_024195" in other vectors (4)
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CNY 3,936.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2810034J18Rik; B5+B5R; b5/b5r; b5b5r; C79736; Cb5cb5r; Ncb5or |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216118 representing NM_024195
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAACGTCCCTTCCCAGGCTTTCCCGGCCCCCGGCTCCCAGCAGCGCGTCTCCTCCCAGGGGCGCA GCAAGGTACCTTTAAAACAAGGCAGGAGTCTTATGGATTGGATCCGACTGACCAAGAGTGGAAAGGACTT AACAGGATTGAAGGGCGGGTTAATTGAAGTAACTGAAGAAGAGCTTAAGAAGCACAACAAGAAAGAAGAT TGTTGGATATGCATAAGAGGGTTTGTTTACAACGTCAGCCCTTATATGGAGTACCATCCGGGTGGGGAGG ATGAACTCATGAGAGCCGCAGGAGCCGATGGAACAGATCTTTTTAATGAGGTTCATCGTTGGGTCAATTA TGAATCCATGCTGAAAGAATGCCTGGTTGGCAGGATGGCTGTGAAGCCTGCTGTTCCAAAAGACTGTCAT GAAGGAAAGAGAGTCTTAAATGGCATGCTTCCCAAGAGCCAAATGTCAGACACACTGCCCAGGGATGTGA CAGACACACTGCCCAGGGAAGACCTCAGTTCTCCAAGCTATGATTGGTTCCAAACAGAGTCTTCAGTCAC CATTGTTGTATACACCAAACAAAAGAATATCAGTTTAGACTCAGTAATCGTTGACCTCCAGGATGACTCC TTGAGAGCAGAAGCAGTCATCAAGGATCACTCTTACCTTGTACATGTTGGGCTAAGCCATGAAGTTCAAG AAAATTTTTCTGTACGGGTTATTGAAAATGTTGGAAAAATAGAGATTGTCCTACAAAAGAAAGAAAGTGT TTCTTGGCAATGTCTTGGTGATCACCTGGAGAAACATGATTCTTTCATTCCAAAGAAAGATACAGGCTTG TACTACAGACGGTGCCAGTTAATATCTAAGGAAGATGTCACTCATGACACGAGACTTTTCTGTCTGATGC TGCCTCCAAGTACTCACCTTCAGGTGCCAGTTGGACAACATGTTTACCTCAAGCTCAGTGTCACAGGTGC AGAAATAGTAAAGCCATATACACCCGTGTCTGATTCCTTACTCTCAGATTTTAAAGAACCAGTCCTTTCC CCAAATAAATACATCTGCTTTTTGATCAAAATCTATCCTGCTGGACTCTTCACACCAGAGCTTGATCGTC TTCAGATTGGAGATTTTATTTCTGTAAGCGGACCTGAAGGCGATTTTAAAGTATCCAAACTCCAGGAAGT AGAAGATCTCTTTCTATTGGCAGCCGGAACAGGATTCACACCAATGGTCACTGTACTGAATTATGCTTTG TCTCACATGTCCAGTCTCAGGAAGGTGAAGTTGATGTTCTTCAATAAAACAGAGGACGATATAATTTGGA GATGTCAGCTAGAGAAGTTGGCATTAAGAGAAAAAAGATTTGATGTTGAATTTGTTCTCTCGGCACCTTC TCCAGAATGGAATGGCAAACAGGGACACATATCACGAGCTCTTCTCTCTGAATTTTTGCAAAGAAGTTCA GAAAACTCTAGGGCCTTCCTTTGCATCTGCGGACCAACACCATTTACTGACGAGGGGATAAGGTTGCTGC ATGATCTTAACTTTTCTGATGATGAGATTCACGGATTCACCGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216118 representing NM_024195
Red=Cloning site Green=Tags(s) MLNVPSQAFPAPGSQQRVSSQGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGGLIEVTEEELKKHNKKED CWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKDCH EGKRVLNGMLPKSQMSDTLPRDVTDTLPREDLSSPSYDWFQTESSVTIVVYTKQKNISLDSVIVDLQDDS LRAEAVIKDHSYLVHVGLSHEVQENFSVRVIENVGKIEIVLQKKESVSWQCLGDHLEKHDSFIPKKDTGL YYRRCQLISKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGAEIVKPYTPVSDSLLSDFKEPVLS PNKYICFLIKIYPAGLFTPELDRLQIGDFISVSGPEGDFKVSKLQEVEDLFLLAAGTGFTPMVTVLNYAL SHMSSLRKVKLMFFNKTEDDIIWRCQLEKLALREKRFDVEFVLSAPSPEWNGKQGHISRALLSEFLQRSS ENSRAFLCICGPTPFTDEGIRLLHDLNFSDDEIHGFTA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024195 |
| ORF Size | 1584 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024195.2, NP_077157.2 |
| RefSeq Size | 2653 bp |
| RefSeq ORF | 1587 bp |
| Locus ID | 266690 |
| UniProt ID | Q3TDX8 |
| MW | 59.7 kDa |
| Gene Summary | NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217526 | Cyb5r4 (untagged) - Mouse cytochrome b5 reductase 4 (Cyb5r4), (10ug) |
CNY 3,936.00 |
|
| MG216118 | Cyb5r4 (tGFP-tagged) - Mouse cytochrome b5 reductase 4 (Cyb5r4), (10ug) |
CNY 5,040.00 |
|
| MR216118L3 | Lenti ORF clone of Cyb5r4 (Myc-DDK-tagged) - Mouse cytochrome b5 reductase 4 (Cyb5r4) |
CNY 6,460.00 |
|
| MR216118L4 | Lenti ORF clone of Cyb5r4 (mGFP-tagged) - Mouse cytochrome b5 reductase 4 (Cyb5r4) |
CNY 6,460.00 |
