Diras2 (NM_001024474) Mouse Tagged ORF Clone

CAT#: MR216101

  • TrueORF®

Diras2 (Myc-DDK-tagged) - Mouse DIRAS family, GTP-binding RAS-like 2 (Diras2)

ORF Plasmid: DDK tGFP



  "NM_001024474" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2900052J15Rik; AI414999
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR216101 representing NM_001024474
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGAACAGAGCAACGACTACCGGGTGGCGGTGTTTGGGGCAGGCGGTGTTGGCAAGAGCTCCCTCG
TTTTGAGGTTTGTGAAAGGGACCTTCAGGGAGAGCTACATCCCGACTGTGGAAGACACCTACCGGCAGGT
GATCAGCTGTGACAAGAGCATATGCACGCTGCAGATCACCGACACCACCGGGAGCCACCAGTTTCCGGCC
ATGCAGCGGCTGTCCATCTCCAAAGGCCACGCCTTCATCCTGGTCTACTCCATCACCAGCCGGCAGTCCC
TGGAGGAACTCAAGCCCATCTATGAGCAGATCTGCGAGATCAAGGGGGATGTGGAGAGCATCCCCATCAT
GCTGGTGGGTAACAAGTGTGATGAGAGCCCCAACCGTGAGGTCCAGAGCAGCGAGGCAGAGGCCCTAGCC
CGCACATGGAAGTGCGCCTTCATGGAGACGTCCGCCAAGCTCAACCACAACGTGAAGGAACTCTTTCAGG
AACTCCTCAACCTGGAGAAGCGCAGGACCGTGAGCCTCCAGATCGACGGCAAGAAGAGCAAGCAGCAGAA
GAGGAAGGAGAAGCTCAAGGGCAAGTGCGTGGTCATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR216101 representing NM_001024474
Red=Cloning site Green=Tags(s)

MPEQSNDYRVAVFGAGGVGKSSLVLRFVKGTFRESYIPTVEDTYRQVISCDKSICTLQITDTTGSHQFPA
MQRLSISKGHAFILVYSITSRQSLEELKPIYEQICEIKGDVESIPIMLVGNKCDESPNREVQSSEAEALA
RTWKCAFMETSAKLNHNVKELFQELLNLEKRRTVSLQIDGKKSKQQKRKEKLKGKCVVM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001024474
ORF Size 597 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001024474.2, NP_001019645.1
RefSeq Size 4348 bp
RefSeq ORF 600 bp
Locus ID 68203
UniProt ID Q5PR73
MW 22.9 kDa
Gene Summary Displays low GTPase activity and exists predominantly in the GTP-bound form.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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