Prpf4b (NM_013830) Mouse Tagged ORF Clone
CAT#: MR216087
- TrueORF®
Prpf4b (Myc-DDK-tagged) - Mouse PRP4 pre-mRNA processing factor 4 homolog B (yeast) (Prpf4b)
ORF Plasmid: tGFP
"NM_013830" in other vectors (4)
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CNY 7,016.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2610037H07; cbp143; Prp4; Prp4k; Prpk |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216087 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCACGGAGCCCCCGTCCCTGCGGGAGCAAGCTGAGATGGATGATGCTGATAATTCTGAAAAGA GTGTAAATGAAGAAAATGGAGAGGTATCAGAAGACCAATCTCAAAATAAACACAGTCGTCACAAAAAAAA GAAGCATAAACACAGAAGTAAACATAAGAAACATAAACATTCCTCAGAAGAAGATAGGGATAAAAAACAC AAACATAAGCATAAGCATAAGAAGCACAAACGGAAAGAAGTTATCGAGGCTTCCGACAAAGAAGGCCTGT CCCCTGCAAAAAGAACTAAGCTTGATGATTTAGCTTTGCTGGAAGACTTGGAAAAGCAGAGAGCCTTGAT TAAAGCTGAGCTTGATAATGAGTTAATGGAGGGGAAGGTCCAGTCTGGAATGGGGCTCATTTTGCAAGGC TATGAGTCTGGCTCCGAAGAAGAAGGAGAGATTCACGAAAAGGCAAGAAATGGGAACAGGTCCAGTACCA GGTCTTCCAGTACTCGGGGAAAACTTGAAATTACGGACAACAAAAATAGTGCAAAGAAACGAAGTAAGAG CAGATCCAAAGAACGGACCAGGCACAGATCGGACAAAAGGAAAAGCAAGGGGGCTGGGGAAATGCTGAGA GAGAAAGCAAACAGGAGCAAGTCCAAGGAGAGGAGAAAATCCAAAAGCCCATCCAAAAGAAGCAAGTCTC AAGACCAAGCTAGAAAGTCAAAGTCCCCTCCACTCAGACGGCGGTCCCAAGAGAAAGTGGGGAAGGCCAG ATCTCCTGCTGAGGAGAAGATGAAGAGTGAAGAGAAAGGCAAAATAAAGGATAGGAAAAAATCACCAATT GTAAATGAAAGGAGTCGAGATAGATCTAAAAAATCCAAGTCCCCAGTTGACTTAAGAGATAAATCCAAAG ACAGAAGGTCAAGGTCCAAAGAGCGGAAGTCAAAACGATCTGAAATTGACAAAGAAAAGAAGCCAATTAA ATCACCTTCAAAGGATGCTTCTTCTGGGAAGGAAAATAGGTCACCCAGTAGAAGACCTGGCCGTAGTCCT AAAAGAAGAAGTTTGTCTCCAAAACTGCGAGACAAGTCAAGAAGAAGTAGGTCTCCGCTTCTCAATGACA GAAGATCTAAGCAGAGCAAATCGCCTTCTCGAACTCTGTCTCCAGGCAGAAGGGCCAAGAGCCGATCCTT AGAAAGAAAACGGAGAGAACCAGAGAGGAGACGACTTTCTTCTCCGAGAACAAGACCTCGAGATGATATC CTTGGTAGATGTGAACGGTCAAAAGATGCCAGCCCCATCAATAGGTGGTCTCCAACCCGAAGAAGAAGTA GGTCTCCAATTAGAAGGAGGTCCCGTTCCCCATTGAGACGCAGCAGGTCTCCTAGAAGAAGAAGCAGGTC TCCTCGGAGAAGAGATAGGAGCCGAAGGAGCAGGTCACGCTTGAGGAGAAGGTCTCGATCACGGGGTGGT CATAGGAGAAGGAGCAGAAGCAAAGTAAAGGAAGATAAATTTAAAGGAAGTCTCTCAGAAGGAATGAAAG TTGAGCAAGAGTCTTCATCTGATGATAACCTTGAAGATTTTGATGTGGAGGAGGAAGATGAAGAGGCTCT AATAGAACAGAGAAGAATACAGAGGCAAGCAATTGTGCAGAAATATAAATATCTTGCTGAAGATAGCAAT ATATCTGTGCCATCTGAGCCAAGCAGCCCCCAGAGCAGCACAAGATCACGATCTCCTTCGCCAGACGACA TCTTGGAGCGAGTTGCTGCTGATGTAAAAGAATATGAGCGGGAGAATGTTGACACCTTTGAGGCCTCAGT AAAAGCCAAGCATAATCTAATGACAGTTGAACAGAATAATGGCTCATCTCAGAAGAAGATTTTGGCACCT GACATGTTTACAGAATCTGATGACATGTTTGCTGCTTATTTTGATAGTGCTCGTCTCCGAGCTGCTGGCA TTGGGAAAGATTTCAAAGAAAATCCCAACCTCAGAGATAATTGGACAGATGCAGAAGGCTATTACCGTGT GAACATCGGGGAAGTCCTAGATAAACGTTATAATGTGTATGGCTACACTGGGCAGGGTGTGTTCAGCAAT GTTGTGCGTGCCAGGGATAATGCGAGAGCCAACCAGGAAGTGGCTGTAAAGATCATCAGGAACAATGAAC TCATGCAAAAGACTGGTTTAAAAGAGCTAGAATTCTTGAAAAAACTTAATGATGCTGATCCTGATGACAA GTTCCATTGCCTGCGCCTCTTCAGACACTTTTACCACAAACAGCACCTCTGTCTCGTCTTTGAACCTCTC AGTATGAATTTGCGAGAGGTATTAAAAAAATATGGTAAGGATGTCGGTCTCCATATTAAGGCTGTACGAT CGTACAGTCAGCAGTTGTTCCTGGCATTGAAACTCCTTAAAAGATGTAATATCTTACATGCAGATATCAA ACCGGATAATATTCTGGTTAATGAATCCAAAACAATTTTAAAACTTTGTGATTTTGGATCGGCCTCACAT GTTGCAGATAATGACATTACGCCTTATCTTGTCAGTAGATTTTATCGTGCTCCGGAGATCATTATAGGTA AAAGCTATGACTACGGTATCGATATGTGGTCTGTAGGTTGTACCTTATATGAACTCTACACTGGAAAAAT CTTATTCCCTGGCAAAACTAATAACCATATGTTGAAGCTCGCCATGGACCTTAAAGGAAAAATGCCAAAT AAGATGATCCGGAAAGGTGTATTCAAAGATCAACATTTTGACCAAAATCTGAACTTCATGTACATAGAGG TTGATAAAGTGACAGAGAGGGAGAAAGTTACTGTCATGAGCACCATTAATCCAACTAAGGACCTGTTGGC TGACTTGATTGGGTGCCAGCGACTTCCTGAAGATCAACGTAAAAAAGTACACCAGCTAAAGGACTTGTTG GACCAGATCCTAATGTTGGACCCAGCTAAGCGAATTAGCATCAACCAGGCCCTGCAGCATGCATTCATCC AGGAAAAAATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216087 protein sequence
Red=Cloning site Green=Tags(s) MAATEPPSLREQAEMDDADNSEKSVNEENGEVSEDQSQNKHSRHKKKKHKHRSKHKKHKHSSEEDRDKKH KHKHKHKKHKRKEVIEASDKEGLSPAKRTKLDDLALLEDLEKQRALIKAELDNELMEGKVQSGMGLILQG YESGSEEEGEIHEKARNGNRSSTRSSSTRGKLEITDNKNSAKKRSKSRSKERTRHRSDKRKSKGAGEMLR EKANRSKSKERRKSKSPSKRSKSQDQARKSKSPPLRRRSQEKVGKARSPAEEKMKSEEKGKIKDRKKSPI VNERSRDRSKKSKSPVDLRDKSKDRRSRSKERKSKRSEIDKEKKPIKSPSKDASSGKENRSPSRRPGRSP KRRSLSPKLRDKSRRSRSPLLNDRRSKQSKSPSRTLSPGRRAKSRSLERKRREPERRRLSSPRTRPRDDI LGRCERSKDASPINRWSPTRRRSRSPIRRRSRSPLRRSRSPRRRSRSPRRRDRSRRSRSRLRRRSRSRGG HRRRSRSKVKEDKFKGSLSEGMKVEQESSSDDNLEDFDVEEEDEEALIEQRRIQRQAIVQKYKYLAEDSN ISVPSEPSSPQSSTRSRSPSPDDILERVAADVKEYERENVDTFEASVKAKHNLMTVEQNNGSSQKKILAP DMFTESDDMFAAYFDSARLRAAGIGKDFKENPNLRDNWTDAEGYYRVNIGEVLDKRYNVYGYTGQGVFSN VVRARDNARANQEVAVKIIRNNELMQKTGLKELEFLKKLNDADPDDKFHCLRLFRHFYHKQHLCLVFEPL SMNLREVLKKYGKDVGLHIKAVRSYSQQLFLALKLLKRCNILHADIKPDNILVNESKTILKLCDFGSASH VADNDITPYLVSRFYRAPEIIIGKSYDYGIDMWSVGCTLYELYTGKILFPGKTNNHMLKLAMDLKGKMPN KMIRKGVFKDQHFDQNLNFMYIEVDKVTEREKVTVMSTINPTKDLLADLIGCQRLPEDQRKKVHQLKDLL DQILMLDPAKRISINQALQHAFIQEKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013830 |
ORF Size | 3024 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013830.3 |
RefSeq Size | 4482 bp |
RefSeq ORF | 3024 bp |
Locus ID | 19134 |
UniProt ID | Q61136 |
MW | 117 kDa |
Gene Summary | Has a role in pre-mRNA splicing. Phosphorylates SF2/ASF.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Rab35 Functions in Axon Elongation Are Regulated by P53-Related Protein Kinase in a Mechanism That Involves Rab35 Protein Degradation and the Microtubule-Associated Protein 1B
,Villarroel-Campos, D;Henríquez, DR;Bodaleo, FJ;Oguchi, ME;Bronfman, FC;Fukuda, M;Gonzalez-Billault, C;,
J. Neurosci.
,PubMed ID 27383602
[Prpf4b]
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Documents
Product Manuals |
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223174 | Prpf4b (untagged) - Mouse PRP4 pre-mRNA processing factor 4 homolog B (yeast) (Prpf4b), (10ug) |
CNY 13,020.00 |
|
MG216087 | Prpf4b (tGFP-tagged) - Mouse PRP4 pre-mRNA processing factor 4 homolog B (yeast) (Prpf4b), (10ug) |
CNY 9,220.00 |
|
MR216087L3 | Lenti ORF clone of Prpf4b (Myc-DDK-tagged) - Mouse PRP4 pre-mRNA processing factor 4 homolog B (yeast) (Prpf4b) |
CNY 10,260.00 |
|
MR216087L4 | Lenti ORF clone of Prpf4b (mGFP-tagged) - Mouse PRP4 pre-mRNA processing factor 4 homolog B (yeast) (Prpf4b) |
CNY 10,260.00 |