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Sipa1 (NM_001164482) Mouse Tagged ORF Clone
CAT＃: MR216077
- TrueORF®
Sipa1 (Myc-DDK-tagged) - Mouse signal-induced proliferation associated gene 1 (Sipa1), transcript variant 3
ORF Plasmid: tGFP
"NM_001164482" in other vectors (4)
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CNY 7,232.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Spa1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216077 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGCCGGCGGTGTGGGGAGCCCTCGGCGGGGCATGGCCCCTGCACCTACCGATGACCTCTTTGCCC GTAAGCTTCGCCAACCTGCCCGGCCCCCACTGACACCACATACCTTTGAGCCGAGGCCAGCTCGGGGCCC ACTCTTGCGCAGTGGCAGTGATGCTGGTGAAGTCCGGCCCCCTACACCAGCCAGCCCCCGTGCCCGTGCC CACAGCCACGAGGATGCCAGCCGCCCTGCTGCAACCCCTACTCGGCTCTTCACTGACCCACTGGCACTGC TAGGGTTGCCAGCAGAAGAGCCAGAGCCCACCTTCCCGCCAGTGCTGGAACCCCGGTGGTTTGCTCACTA TGATGTGCAGAGCTTGCTCTTTGACTGGGCTCCACGACCTCGGGGGACAGGCAGCCATATAGAGGCAAAC TCTGGGACCTTAGCTGAGGGCCAGACTACCACCTCAGATCTACTGCTCGGGGCACCTGGCTTTGTGAGCG AGCTTGGTGGTGAGGGTGAGCTAGGGCTGGGTGGGCCAATATCCCCACCTGTGCCCCCTGCACTGCCTAA TGCGGCTGTGTCCGTCCTGGAGGAGCCACAGACCCGGACCACAGCTTACAGCCTGGAGCACGCAGATCTG GGTGCAGGCTACTACCGCAAGTACTTCTATGGCAAAGAACACCAGAACTTCTTTGGGCTGGATGAGGCGC TGGGTCCGGTGGCCGTGAGCCTGCGACGGGAGGAGAAAGAGGGCAGCGGAGGGGGCACCCTACACAGCTA CCGGGTCATCGTGCGGACCACGCAGCTCCGGACCCTCCGTGGCACCATCTCGGAGGACGCACTGCCTCCC GGCCCCCCGAGGGGCCTATCTCCGAGGAAGCTTCTGGAACATGTGGCTCCACGGCTGAGCCCCACCTGCC TGCGCCTGGGTTCAGCCTCTCCCAAGGTGCCCCGCACGCTGCTTACTCTGGATGAGCAAGTGCTGAGCTT CCAACGCAAGGTGGGCATCCTGTACTGCCGCGCAGGCCAGGGCTCTGAGGAAGAGATGTACAACAACCAG GAGGCCGGAGCAGCCTTTATGCAGTTCCTTACCTTGCTGGGTGATGTGGTGCGACTCAAAGGCTTTGAAA GTTACCGGGCCCAGCTGGATACCAAAACGGATTCCACGGGCACACACTCACTCTACACCACCTACCAAGA CCATGAGATCATGTTTCACGTGTCCACGATGCTGCCTTACACGCCTAATAACCAGCAACAGCTCCTGAGG AAGCGTCATATCGGCAACGATATTGTGACCATCGTGTTCCAGGAGCCCGGTAGCAAGCCCTTCTGCCCTA CGACAATCCGCTCTCACTTCCAGCACGTTTTCTTGGTGGTGCGTGCGCATGCTCCCTGCACCCCACACAC CTCATACAGGGTGGCAGTGAGCCGCACCCAGGACACTCCTGCCTTCGGTCCTGCGCTGCCAGAAGGCGGA GGCCCCTTTGCAGCCAATGCCGATTTCCGGGCCTTTCTGTTGGCTAAGGCACTCAATGGTGAGCAAGCGG CTGGTCATGCACGCCAGTTCCACGCCATGGCTACACGCACACGCCAACAGTACCTGCAGGACCTGGCTAC TAATGAAGTGACCACTACTTCGCTGGACTCGGCTTCGCGGTTTGGCCTGCCATCTCTGGGGGGTAGGCGC CGGGCAACCCCTCGGAGCCCAGGCGCGGAGCTACAGGCGGCGGGTGCGCTGATGTGGGGCGTACGCGCGG CTCCAGGGGCGCGGGTCGCAGCGGGAGCTGAAACGAGCGGTCCGGAGGACGCCGAGGTGCCCTGCTTGTT GGGCATCTCAGCAGAGACACTGGTGCTGGTGGCACCTCGCGACGGCCGCGTGGTCTTCAATTGTGCCTGT CGCGACGTATTGGCCTGGACCTTCTCAGAGCACCAACTCGATCTGTACCACGGGCGCGGGGAGGCGATCA CGCTGCGGCTCGACGGGGCCCCAGGGCAAGCCGTGGGCGAAGTCGTGGCACGTCTGCAGCTGGTGAGCCG CGGGTGTGAGACCAGAGAACTAGCGCTGCCCAGAGATGGCCAAGGTCGCCTGGGCTTCGAGGTGGATGCA GAAGGCTTCATCACGCACGTGGAGCGCTTCACGTTTGCGGAGACCACGGGGCTTCGGCCTGGAGCTCGTT TGCTGCGAGTCTGCGGCCAGACGCTGCCCAAGCTGGGTCCCGAAGCTGCTGCCCAGATGCTGCGCTCTGC GCCCAAGGTCTGCGTCACGGTCCTACCCCCAGACGAGAGCGGCCGGCCGCGAAGGAGCTTTTCGGAGCTC TATATGCTCTCTCTGAAGGAACCCAGCCGGCGGGGGGGTCCAGAGCCAGTACAGGATGAAACTGGGAAGT TGGTCATATTGCCTCCCACCAAGCAGCTGCTACATTTTTGCCTGAAAGACAGCAGCAGTCCTCCGGGGCC TGGGGATCTGACTGAGGAGAGGACAGAGTTCCTGCGCAGCCACAACTCCCTGTCATCTGGAAGCTCCCTG TCCGATGAGGCTCCAGTCCTGCCCAACACCACTCCAGACCTCCTCCTTGTCACCACTGCCAACCCATCTG CACCTGGTACTGACAGAGAAACACCCCCTTCCCAGGACCAGTCAGGAAGCCCCAGTAGCCATGAAGACAC CAGTGACTCAGGCCCAGAACTGAGGGCCTCCATCCTGCCCAGAACCTTGTCTCTGCGGAATTCCATCAGT AAGATTATGTCGGAAGCTGGCAGTGAGACCCTGGAGGATGAGTGGCAGTCCATCTCAGAGATCGCCTCCA CTTGCAACACAATTCTGGAGTCACTGTCCCGGGAGGGACAACCCATCTCAGAGAGCGGAGACCCCAAGGA AGCTTTAAAGTGTGATTCTGAGCCAGAACCCGGGAGCCTGTCAGAAAAGGTCTCTCACCTAGAGTCCATG CTCTGGAAGCTCCAGGAGGACCTGCAGAGGGAGAAGGCGGACAGGGCAGCCTTGGAGGAGGAGGTTCGGA GCCTCAGACACAACAACCAGAGGCTGCTGGCAGAGTCCGAGAGTGCCGCCACCCGCCTGCTCCTGGCCTC TAAGCATCTGGGTGCACCCACTACTGACCTGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216077 protein sequence
Red=Cloning site Green=Tags(s) MWAGGVGSPRRGMAPAPTDDLFARKLRQPARPPLTPHTFEPRPARGPLLRSGSDAGEVRPPTPASPRARA HSHEDASRPAATPTRLFTDPLALLGLPAEEPEPTFPPVLEPRWFAHYDVQSLLFDWAPRPRGTGSHIEAN SGTLAEGQTTTSDLLLGAPGFVSELGGEGELGLGGPISPPVPPALPNAAVSVLEEPQTRTTAYSLEHADL GAGYYRKYFYGKEHQNFFGLDEALGPVAVSLRREEKEGSGGGTLHSYRVIVRTTQLRTLRGTISEDALPP GPPRGLSPRKLLEHVAPRLSPTCLRLGSASPKVPRTLLTLDEQVLSFQRKVGILYCRAGQGSEEEMYNNQ EAGAAFMQFLTLLGDVVRLKGFESYRAQLDTKTDSTGTHSLYTTYQDHEIMFHVSTMLPYTPNNQQQLLR KRHIGNDIVTIVFQEPGSKPFCPTTIRSHFQHVFLVVRAHAPCTPHTSYRVAVSRTQDTPAFGPALPEGG GPFAANADFRAFLLAKALNGEQAAGHARQFHAMATRTRQQYLQDLATNEVTTTSLDSASRFGLPSLGGRR RATPRSPGAELQAAGALMWGVRAAPGARVAAGAETSGPEDAEVPCLLGISAETLVLVAPRDGRVVFNCAC RDVLAWTFSEHQLDLYHGRGEAITLRLDGAPGQAVGEVVARLQLVSRGCETRELALPRDGQGRLGFEVDA EGFITHVERFTFAETTGLRPGARLLRVCGQTLPKLGPEAAAQMLRSAPKVCVTVLPPDESGRPRRSFSEL YMLSLKEPSRRGGPEPVQDETGKLVILPPTKQLLHFCLKDSSSPPGPGDLTEERTEFLRSHNSLSSGSSL SDEAPVLPNTTPDLLLVTTANPSAPGTDRETPPSQDQSGSPSSHEDTSDSGPELRASILPRTLSLRNSIS KIMSEAGSETLEDEWQSISEIASTCNTILESLSREGQPISESGDPKEALKCDSEPEPGSLSEKVSHLESM LWKLQEDLQREKADRAALEEEVRSLRHNNQRLLAESESAATRLLLASKHLGAPTTDLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001164482 |
| ORF Size | 3114 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001164482.1, NP_001157954.1 |
| RefSeq Size | 3553 bp |
| RefSeq ORF | 3117 bp |
| Locus ID | 20469 |
| MW | 112.2 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223325 | Sipa1 (untagged) - Mouse signal-induced proliferation associated gene 1 (Sipa1), transcript variant 3, (10ug) |
CNY 13,400.00 |
|
| MG216077 | Sipa1 (tGFP-tagged) - Mouse signal-induced proliferation associated gene 1 (Sipa1) transcript variant 3, (10ug) |
CNY 9,220.00 |
|
| MR216077L3 | Lenti ORF clone of Sipa1 (Myc-DDK-tagged) - Mouse signal-induced proliferation associated gene 1 (Sipa1), transcript variant 3 |
CNY 10,260.00 |
|
| MR216077L4 | Lenti ORF clone of Sipa1 (mGFP-tagged) - Mouse signal-induced proliferation associated gene 1 (Sipa1), transcript variant 3 |
CNY 10,260.00 |
