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Dus3l (NM_144858) Mouse Tagged ORF Clone
CAT＃: MR216052
- TrueORF®
Dus3l (Myc-DDK-tagged) - Mouse dihydrouridine synthase 3-like (S. cerevisiae) (Dus3l)
ORF Plasmid: tGFP
"NM_144858" in other vectors (4)
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CNY 6,370.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI662135; AW557805 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216052 representing NM_144858
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGACGGCAGCGGAGAGCGGTGGTGGCGGTGACTCCGGGGTTGGCGCGTGCGAACGGGGGGTGG CGCCCATTAAAGCTCAGTACCGCACAACCAAAGAACGGTTCCACGAATATCTGGATGGAGACAAGCAGGA GGGTGCATGCCAGGAAGTGCCCACAGGAGACCCAGCTGAGCCAGGGGCCAAAAGAATTCGCCTGGAAGAT GGGCAGGAAAATGGGAAGACAGAGGTGGCAATTGAATCCCGTGAAAGGCAGGTGCCCAAGAGGGCCCGTG GCCAAAACAAAAGCCGGCCCCATGTGAAGCCAGCACACTACGACAAGGACAGGCTCTGCCCGTCCTTCCT CCAGGAGCCGGCCACCCCATGTGCATTTGGAGACCGTTGCCGCTTCCTGCACGATGTGGGGCGCTACCTG GAGACCAAACCGGCAGACCTGGGCCCCCGCTGCGTGCTCTTCGAAACCTTTGGCCGGTGCCCTTTCAGCA TGACCTGTCGCTTTGCTGGAGCACACCTGGGGCCTGAGGGGCAGAACCTGGTGCAGGAAGAGGTGGTGGC CCGCTGTGCACAGCTCCCTTCAGTGCGGAATGGCTTAGACAGGGCCCTGCAGCAGCAGCTGCGGAAGCGC CAAGTGTGCTTTGAGCGAGCTGAGCAGGCCCTGAACCGCCTCACCCAGAGCCCCATGCCCACTGTTGTCC CTGAGACCACTGTGGCCATGGCGACCCCAAAGCAGAATAGCTGCCATGCCCAGCTAGATACCGTAGGAGG GGCTGGCACCCCGCAGAGCAGCCCTGTGCCAACCTGTGGCCCCTTGACAGACGAGGACGTGATCCGGCTG CGGCCCTGTGAGAAGAAGCGGCTAGACATCAGCGGAAAGCTGTATCTGGCTCCTTTAACCACGTGTGGAA ACCTACCCTTTCGCCGCATTTGCAAGCGCTTTGGCGCGGATGTGACCTGCGGTGAGATGGCCGTGTGTAC GAACCTGCTCCAGGGACAGATGTCTGAGTGGGCTTTGCTCAAGCGCCACCCCTGTGAGGACATCTTTGGG GTGCAGCTGGAGGGCGCGTTCCCTGACACCATGACCAAATGTGCCGAGCTCCTCAACCGCACCATTGACG TGGACTTCGTGGACATCAACGTGGGCTGCCCCATCGACCTCGTCTACAAGAAGGGTGGTGGCTGTGCACT CATGAATCGCTCCGCCAAGTTCCAACAGATTGTGCGGGGCGTGAATGAGGTGTTGGACGTCCCACTGACT GTGAAGATGCGCACAGGTGTCCAGGAGCGTGTGAGCCTGGCACACCGACTGCTGCCCGAACTGCGGGACT GGGGTGTCGCACTGGTCACGCTCCATGGCCGCTCCCGGGAACAGCGCTACACCAGGCTGGCTGACTGGCC CTACATAGAACAGTGTGCCAAGGTGGCCAGCCCCATGCCCCTGTTTGGAAATGGGGACATCCTTTCGTTT GAGGACGCCAACTGTGCCATGCAGACAGGCGTTGCGGGGATCATGGTTGCCCGTGGTGCCTTGCTCAAGC CCTGGCTGTTCACGGAGATCAAAGAACAGAGGCACTGGGACATCTCGTCCTCCGAGCGCCTGGACATCCT GAGGGACTTCACTCACTACGGTTTAGAACACTGGGGCTCCGACACACAGGGCGTGGAGCGGACCCGGCGC TTCCTGCTTGAGTGGCTCTCTTTTCTTTGCAGGTATGTGCCCGTGGGCCTGCTCGAGCGACCCCCACAGA GGATCAATGAGAGGCCACCCTATTACTTAGGCCGTGACTACCTGGAGACACTGATGGCCAGCCAGCAGGC TGCAGACTGGATTCGCATCAGTGAGATGCTGCTTGGACCAGTACCTCCTGGCTTCGTCTTCCTGCCCAAG CACAAGGCCAATGCCTACAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216052 representing NM_144858
Red=Cloning site Green=Tags(s) MAETAAESGGGGDSGVGACERGVAPIKAQYRTTKERFHEYLDGDKQEGACQEVPTGDPAEPGAKRIRLED GQENGKTEVAIESRERQVPKRARGQNKSRPHVKPAHYDKDRLCPSFLQEPATPCAFGDRCRFLHDVGRYL ETKPADLGPRCVLFETFGRCPFSMTCRFAGAHLGPEGQNLVQEEVVARCAQLPSVRNGLDRALQQQLRKR QVCFERAEQALNRLTQSPMPTVVPETTVAMATPKQNSCHAQLDTVGGAGTPQSSPVPTCGPLTDEDVIRL RPCEKKRLDISGKLYLAPLTTCGNLPFRRICKRFGADVTCGEMAVCTNLLQGQMSEWALLKRHPCEDIFG VQLEGAFPDTMTKCAELLNRTIDVDFVDINVGCPIDLVYKKGGGCALMNRSAKFQQIVRGVNEVLDVPLT VKMRTGVQERVSLAHRLLPELRDWGVALVTLHGRSREQRYTRLADWPYIEQCAKVASPMPLFGNGDILSF EDANCAMQTGVAGIMVARGALLKPWLFTEIKEQRHWDISSSERLDILRDFTHYGLEHWGSDTQGVERTRR FLLEWLSFLCRYVPVGLLERPPQRINERPPYYLGRDYLETLMASQQAADWIRISEMLLGPVPPGFVFLPK HKANAYK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144858 |
| ORF Size | 1911 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144858.2, NP_659107.2 |
| RefSeq Size | 2321 bp |
| RefSeq ORF | 1914 bp |
| Locus ID | 224907 |
| UniProt ID | Q91XI1 |
| MW | 71.6 kDa |
| Gene Summary | Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220035 | Dus3l (untagged) - Mouse dihydrouridine synthase 3-like (S. cerevisiae) (Dus3l), (10ug) |
CNY 8,270.00 |
|
| MG216052 | Dus3l (tGFP-tagged) - Mouse dihydrouridine synthase 3-like (S. cerevisiae) (Dus3l), (10ug) |
CNY 7,030.00 |
|
| MR216052L3 | Lenti ORF clone of Dus3l (Myc-DDK-tagged) - Mouse dihydrouridine synthase 3-like (S. cerevisiae) (Dus3l) |
CNY 8,270.00 |
|
| MR216052L4 | Lenti ORF clone of Dus3l (mGFP-tagged) - Mouse dihydrouridine synthase 3-like (S. cerevisiae) (Dus3l) |
CNY 8,270.00 |
