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Kng1 (NM_001102412) Mouse Tagged ORF Clone
CAT＃: MR215632
- TrueORF®
Kng1 (Myc-DDK-tagged) - Mouse kininogen 1 (Kng1), transcript variant 3
ORF Plasmid: tGFP
"NM_001102412" in other vectors (3)
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CNY 4,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Kng |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR215632 representing NM_001102412
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTCATTACTACACTGCTCCTCTGCTCCGGACTCCTGCTGACTTTAACACAGGGAGAAGAAGCGC AGGAAATTGACTGCAATGATGAGGCTGTATTTCAGGCTGTGGATTTCTCTCTGAAGCAGTTTAACCCTGG GGTAAAAAGTGGCAACCAGTATATGTTGCACCGAGTGATCGAGGGCACTAAAACGGATGGCTCTCCAACC TTTTACTCCTTCAAGTATCTAATCAAGGAGGGCAACTGCTCTGCTCAGAGTGGCCTCGCATGGCAGGACT GTGACTTCAAGGACGCTGAGGAAGCCGCCACTGGAGAATGCACAGCAACTGTGGGGAAAAGAGAAAATGA ATTCTTCATAGTCACCCAGACCTGCAAGATTGCTCCAAGTAAGGCCCCCATACTGAAAGCCTATTTCCCC TGTATTGGTTGTGTGCATGCCATATCGACAGATAGTCCAGACCTGGAGCCTGTTCTGAAACACTCCATCG AACATTTCAACAACAACACAGATCACAGCCACCTCTTTACTCTCAGAAAAGTAAAAAGTGCCCACAGACA GGTGGTGGCTGGCCTGAATTTTGACATTACCTACACAATTGTGCAAACAAATTGTTCAAAGGAGCGTTTT CCTTCCCTCCATGGAGACTGCGTGGCCCTTCCCAATGGTGATGATGGTGAATGTAGAGGAAATCTCTTCA TGGATATTAATAACAAAATTGCCAACTTCTCACAGAGCTGTACCCTTTATTCAGGAGATGATTTGGTAGA AGCGCTTCCCAAGCCTTGCCCTGGCTGCCCCAGGGACATACCTGTAGACAGCCCAGAGCTGAAGGAGGTG CTTGGTCATTCCATTGCACAGCTAAATGCAGAGAATGACCATCCTTTCTATTACAAGATTGACACCGTGA AAAAAGCAACATCACAGGTGGTAGCAGGAACTAAATATGTTATTGAGTTCATAGCCAGAGAAACCAAATG CTCCAAGGAAAGTAACACAGAGCTGGCAGAAGATTGTGAGATCAAGCACCTTGGACAAAGTCTCGACTGC AATGCTAACGTGTACATGAGACCTTGGGAGAACAAAGTCGTCCCGACTGTGAAATGCCAAGCATTAGATA TGACTGAAATGGCAAGAAGGCCTCCAGGTTTTTCTCCTTTCCGGAGTGTCACAGTACAAGAAACAAAAGA AGGAAGAACTGACTCAGATTTCATTGAAGATGTGGTAGCTACCACCCCACCATATGACACTGGGGCCCAT GATGATTTGATCCCTGATATCCATGTACAGCCAGATAGCCTTTCATTTAAGCTGATATCTGATTTTCCAG AAGCAACTTCCCCCAAATGTCCTGGGCGCCCATGGAAGCCAGCTAGCTGGAAGGATCCAAACACAGAAAC AACAGAATTTTCTGATTTTGATCTCCTTGATGCTCTTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215632 representing NM_001102412
Red=Cloning site Green=Tags(s) MKLITTLLLCSGLLLTLTQGEEAQEIDCNDEAVFQAVDFSLKQFNPGVKSGNQYMLHRVIEGTKTDGSPT FYSFKYLIKEGNCSAQSGLAWQDCDFKDAEEAATGECTATVGKRENEFFIVTQTCKIAPSKAPILKAYFP CIGCVHAISTDSPDLEPVLKHSIEHFNNNTDHSHLFTLRKVKSAHRQVVAGLNFDITYTIVQTNCSKERF PSLHGDCVALPNGDDGECRGNLFMDINNKIANFSQSCTLYSGDDLVEALPKPCPGCPRDIPVDSPELKEV LGHSIAQLNAENDHPFYYKIDTVKKATSQVVAGTKYVIEFIARETKCSKESNTELAEDCEIKHLGQSLDC NANVYMRPWENKVVPTVKCQALDMTEMARRPPGFSPFRSVTVQETKEGRTDSDFIEDVVATTPPYDTGAH DDLIPDIHVQPDSLSFKLISDFPEATSPKCPGRPWKPASWKDPNTETTEFSDFDLLDALS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001102412 |
| ORF Size | 1440 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001102412.1, NP_001095882.1 |
| RefSeq Size | 1756 bp |
| RefSeq ORF | 1443 bp |
| Locus ID | 16644 |
| MW | 53.7 kDa |
| Gene Summary | (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG215632 | Kng1 (tGFP-tagged) - Mouse kininogen 1 (Kng1) transcript variant 3, (10ug) |
CNY 5,230.00 |
|
| MR215632L3 | Lenti ORF clone of Kng1 (Myc-DDK-tagged) - Mouse kininogen 1 (Kng1), transcript variant 3 |
CNY 6,650.00 |
|
| MR215632L4 | Lenti ORF clone of Kng1 (mGFP-tagged) - Mouse kininogen 1 (Kng1), transcript variant 3 |
CNY 6,650.00 |
