Parp4 (NM_001145978) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Adprtl1; C030027K23Rik; E230037B21Rik; Gm743; p193; PARPL; PH5P; VAULT3; VPARP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR215472 representing NM_001145978, codon optimized. Due to the complexity of NM_001145978, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATTGGGCATTTTCGCTAACTGCATCTTCTGTCTCAAGGTCAAGTACCTTCCCCGACAGCAGAAAA AAAAGCTCCAAACAGATATTAAGGAAAACGGGGGGAAGTTTTCCTTTCTTCTCAATCCTCAGTGTACCCA CGTCATTGTAGACTCTGCAGACGTCCTCAGTCGGTGCCACCTGAATAGCATCCAGAAGAATGACGTTCAG ATCGCTAACCCTGCATTCATCCAGGATAGCGTACGGCAGAGGAGACTGCTGGACGTTAGGAATTACGATC CCCTGTCCCCAGCACCTGCTGCTCCCCCGGCTGAACGGAGCCGCTCTGAAGTGCAGTCTGAGTACCTTCC CAGCGACAACACCCCAGAAAAGGAGAACACTGAAGTTACTGAGGTGTCTGCTGAGAACGTGGAGATCCCA CCTTTCCTCCAGGACTTCGAGGTTGTGAAATATAATATTCTCGAAAAGGTCGGGGGTCCCGAAACCGTGG TGGTGGAGCTTCAGAGTTCCCAGGACCCTGAAAGCTGCCCGTTTGTCATCACCGCCCATTTCCTGCTCGC CGACCAGAAAACTCGACGGGAGTCAACGGGAAAGCAGACCTCTGAAGGAGCCATCGAATACTACGAGTCA TATGTCGAGGATCTGAAGCGACAGGGGTTCCTGCTGCAGGAGCATTTCACAGCAGAAGCAACTCAGTTGG CCAGCGAAAAACTGCAGGCACTGCTGCTGGAGGAGGTGATCTCCAGCGGGGCATTGAGCCAAGAGGTTTC AGATCTTCTCGAGGTTATCTGGACCGAGGCCCTCGGACATTTGGAGAATACACTCCTTAAGCCTGTTAAT TCTATGAGTTTGAATGATGTGTCTAAGGCTGAGGGAATACTGCTCCTGGTTAAAACAGCACTCAAAAACG GCGATAGCCCTGGGCAGCTGCAGAAGACTATGGCTGAGTTCTATAGGCTCCTCCCGCACAGGCACCCAGC ATCTGAGGAGGTCAATCTCAGGCTGTTGGCTCAAAAAGAGGACCTCTGCCAGTTGGTAAGAGACATGGTC AATGTATGTGAAACCAACCTGAGTAAACCAAATCCCCCTAGCCTCGCCAAGTACCGCGCTTTGAGGTGTA AGATCGAGCATGTTGACCAGAATACCGAAGAGTTCAGCCGGGTCAGGAAGGAAGTGTTGCAGAATAACCG GAGCGAGCAGCCCGTGGACATCCTGCAAATATTCCGGGTGGGCAGGGTAAATGAGGCCACAGAGTTCCTG AGTAAGCTGGGAAATGTTCGCCTGCTGTTTCACGGATCCCCGGTAAGAAACATTCTCGGAATCCTCTCCA GAGGGCTGCTGCTGCCAAAGGTAGCCGAGGACAGGGGTGTTCAGAGAACCGACGTGGGCAACTTGGGGAG CGGGATCTACTTTTCTGATTCCCTGAGCACCAGCATTAAGTACGCCCATGCCGGTGAGACTGACGGCTCC CGGCTGCTCGTAGTGTGTGACGTTGCCCTGGGAAAGTGCGTGAATCTGTTTAAGAAAGATTTCTCCCTGA CAGAAGCCCCTCCAGGGTATGACTCAGTCCATGGAGTTTCCGAAACCACAAGTGTGCCCACCGACTTCCA AGACGATGAGTTCGTGGTATATAAGACTAATCAGGTCAAAATGAAGTACATCGTCAAATTCTGTACCCCT GGGGACCAGATTAAAGAATTTCACCCCCACGAGAATACAGAGGTGGAGGAGCAAAGAGCTGAACCTAGCA GTGTTCCAGAGGCCGGCGACTTTCAGCTGCCCGACATTAAGCCCTTTACAAATATAAAAGCGGGTTTGCA GGATGCCTCCGCTAACCCCGTCCCTCTCGACTCTGTACACATCAAGGGCAGGGTCATCGACTTTGTTGCA CAGGTGATCGTCTTTCAGACTTATACCAACCAGTCCCACGTGCCAATAGAGGCAAAATATATCTTCCCTC TGGATGACAAGGCAGCCGTGTGTGGGTTCGAAGCATTCATAAACGGAAAGCACATAGTTGGGGAAATCAA GGAAAAAGAAGAGGCCCGACAGGAGTACCGAGAGGCCGTCTCTCAGGGCCATGGTGCATACCTGATGGAC CAAGACACCCCTGACGTATTTACTGTTTCAGTCGGGAATCTGCCACCTCGGGCCAAAGTCCTGATTAAGA TCACGTACATCACGGAGCTTTCTATACAATCTCCCGTGGCCATCTTCTTCATTCCAGGAACAGTAGCACC CTGGCAGCAGGACAAAGCGTTGAACGAGAACTTGCAGGATACAGTTGAAACAATCCGCATTAAGGAAATC GGAGCTGAGCAAAGTTTCTCCCTGGCTATGAGCATTGAAATGCCGTACATGATCGAGTTCATCAGTAGTG ACACCCACGAACTGAGACAGAAAAGCACAGATTGTAAAGCAGTCGTCAGCACAGTGGAAGGCAGTAGTCT CGATTCTGGAGGGTTCTCCTTGCATATCGGCCTGCGGGACGCTTACCTGCCTCGCATGTGGGTGGAGAAA CACCCTGAGAAGGAATCTGAGGCCTGCATGCTCGTGTTTCAGCCAGAGCTGGCCGACGTCTTGCCAGACC TGCGGGGAAAGAATGAGGTCATCATCTGCCTGGACTGCAGCTCTTCCATGGAAGGAGTAACCTTTACCCA AGCTAAGCAGGTGGCACTGTATGCCCTCAGTCTCCTGGGGGAAGAACAGAAAGTCAATATTATGCAATTC GGGACCGGGTACAAGGAATTGTTCAGCTACCCCAAGTGTATCACAGACAGTAAGATGGCAACTGAGTTCA TTATGTCCGCCGCACCCTCAATGGGTAATACAGATTTTTGGAAGGTGCTGAGGTATCTGTCACTGCTGTA CCCCAGCGAAGGATTCCGCAATATTCTGCTCATCTCTGACGGCCACCTCCAGTCTGAAAGCCTCACCCTG CAGCTGGTGAAGAGGAACATTCAGCATACGCGAGTCTTCACTTGTGCAGTGGGGAGCACAGCAAACAGGC ACATCCTGCGCACCCTGTCTCAGTGCGGAGCCGGCGTGTTCGAGTATTTTAACAGCAAGAGCAAACACTC TTGGAAGAAGCAGATTGAGGCCCAAATGACAAGGATCAGATCTCCCTCATGTCATTCTGTCAGCGTAAAA TGGCAGCAGCTCTCCCGCGATGCTCCTGAACCCTTGCAGGCCCCTGCATGGGTTCCTAGCCTTTTCCATA ATGATCGCCTGTTGGTTTACGGGTTCATTCCTCATTGTACCCAGGCAACCCTGCAAGCCTTTATTCAGGA GAAAGAGTTTTGTACCATGGTTAGCACCACCGAACTGCAGAAGACCACTGGCACTATGATCCATAAATTG GCAGCACGGGCACTGATCAGGGATTATGAGGACGGGATCCTGCACGATGATGAGACGAACCATGAGATGA AGAAGAACATCATGAAATCTCTGATAATCGAACTTAGCAAGGAGAACAGTCTGATAACACAGTTTACTTC CTTCGTAGCAGTGGAAAAGCGAGATGTGAATGAAATTCCGTTCGCAAACGTCCCTAATATTAGTGAACTG GTCGCAAAGGAGGATGTGGACTTCTTGCCATACGTTTCTTGGCAGGAGAAGCAGCCAGAGGCCAGTATTT CCCAGACCGAAATTGATTCATCACGCCTCAAGCACAATAAACTGTCTGATGGACATGGTGTACTTCAGCC AGTCAGCGTGTCTAGCGAAGTGAACGAAAAACCTAGCCTTCTGCTTGCAGCTAAAAAGCGCAAGATTAAG ACTATTAAGAAATGCAGCCTTGATATCAGCGAAGATTTCGAGGACCGAACTGCTGTGGCCCAGTCCCCCG CAACTGCACAATCCTTGAATTTCCATCTTCCTCTGTCAGTGCGCCCTCAGCTCAAGGCCGTCGAGCAGCA GCTGCACGGCAACAGACTGGAACCCAAACAGCGCGGAGGATTTCGGAAACTCCTGATGGCCAAAAAGTGC AGGAACGTGCCCGACTCCCTTGTCTCCAGCGCCCCTGCTGTTACGGCCGAGTTCTCATATCTGTCCGCAT GCTCCTCATCTTCTGCTTTTCTTAGCCCACTGTGCGACATCCCTTCATCACTGCCCCCCCATCCCCTCGG TGGTACTCATCCCCCCCCTCCACTCCCACTGCCTGACGGGACTCACCTGCCATCTCCACTCTTCGGAAGC ACTCATCCTCCACCACCACTGTTCGGTGGCACTCTCATCCCACCTCCTTCTAGCCTCTTCGGCGGAACAC ACCTTCCACCACCTCCACCCCTGCCTGGGGGAACGCACATACCACCACCTCCACCCATTCCTGGAGGAAC TCTCATCCCTCCCTCTAGTTCCCTTTTCGGCGGGACACATCTGCCTCCTCCGCCTCTCCTGTCCGCAGGC ACACATATTCCACCACCTCCTTTGCTGTCAGCTGGCACGCACTTGCCTCCTCCCCCCCTCCTGCCAGCTG GCACCCACATACCTCCACCACCACCCATCACCGGGTCTACTCACCCACCTCCACCGTCATCTCTGTTCGG GGGCACACATCTGCCGCCCCCACCCCCCCTGCCTGGCGGTACTCACATTCCTCCGCCACCTCCTATACCT GGCGGAACATTGATCCCATCCCCCTCCTCACTGTTCGGCGGAACACACCTTCCCCCTCCCCCTCTTCTCC CTGCGGGAACCCATATCCCGCCGCCCCCTCCCATCACAGGATCTACCCACCCACCCCCCCCCTCCAGCCT GTTCGGGGGGACCCATCTTCCTCCGCCTCCTCCCGCAGGCACTCAGTTCTCTCTGAGCCCCATAGGCTTT ATTCCTCCTAAGCTGGGCCCCCCAAAGCTCAGCCATAGTCACAAGTTGGTCGGCGATACCAATATACATG ACAGCGAGCCACCCCTGTTGGGGTTTAAAGATCTCTGTAGCCGAGACATGGGCTTCTCTTGTGGAACAGC ATTTTCTGGGAGCTTCGCTTCTTCCAAAGACTTCGACCCAGGGAAGTTCAGCCAGGGACCAAACAACATC AGCTTTTCCCCTAAGGCCCCAGAAATGGGAGTGCTTCATCAGTCTCCGTTCTGCAGCCCGCCAAAGCCAC CTTCCGCACCTCCATTGGTGACAAATGTGCTGTGCTCAGAGGCACCTCAATCTTATTTCCTGAATCTTCA GAGCGCCGCTGTTCACCAGTCCCCCAACAACCGCGTGTCAGAGATCATAATGGAATCAGTGGAGAGTTCT TTGCCGTCAGACTATTCTAGCCGGGATGCTTCTAGCTACCTTGCTCTCGAGGGGGCCGAGGACTCTCTCC TGGGTGGGTCCTCCTTTGAAACAGACACCGACGAGGCCGCCGCTTTCATAGCGAATGATCTCCTCACAAG CATTGAAACCAGCTCTGATGAGGAGTGTGCTTTCTGCGATGAGGACCAGGAGTCCCCCGTACCATGGGCA AGCCTTTTCGCACTGCAGACGGAGAACGGGTTCTGGAAGCTTACACCTGAACTTGGTCTGATCCTGAATC TGAACGTGAACGCTCTGCTGACCAGCCTGGAAGAGAAGGGAATTAGGAGCCTTGGCACCAAGGGCCGCGA ACGGCTCCTCGACTTGATAGCTACTCTGCTGGTGCTGCAGTTCCTTTACACCAAGCTGGAGCAAGAGGGA ATGGTGGCAAAAAGTTTGATCAAAATGGATGATGCCTTTATCAGCAGGAACATACCCTGGGCATTCGAAA ATATCAAGAAGGCACGGGAGTGGGCTCGCAAGACAGAGGGCCAGTACCCCTCAATTTGTCAGCGCTTGGA GCTCGGAAAAGACTGGGAATCTGCGACCAAGCAGCTCTTGGGAATCCAGCCTCAGGCAAATACCTCTCTG CACCGCATCCTGTACTACAGCCAGGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR215472 representing NM_001145978 Red=Cloning site Green=Tags(s) MTLGIFANCIFCLKVKYLPRQQKKKLQTDIKENGGKFSFLLNPQCTHVIVDSADVLSRCHLNSIQKNDVQ IANPAFIQDSVRQRRLLDVRNYDPLSPAPAAPPAERSRSEVQSEYLPSDNTPEKENTEVTEVSAENVEIP PFLQDFEVVKYNILEKVGGPETVVVELQSSQDPESCPFVITAHFLLADQKTRRESTGKQTSEGAIEYYES YVEDLKRQGFLLQEHFTAEATQLASEKLQALLLEEVISSGALSQEVSDLLEVIWTEALGHLENTLLKPVN SMSLNDVSKAEGILLLVKTALKNGDSPGQLQKTMAEFYRLLPHRHPASEEVNLRLLAQKEDLCQLVRDMV NVCETNLSKPNPPSLAKYRALRCKIEHVDQNTEEFSRVRKEVLQNNRSEQPVDILQIFRVGRVNEATEFL SKLGNVRLLFHGSPVRNILGILSRGLLLPKVAEDRGVQRTDVGNLGSGIYFSDSLSTSIKYAHAGETDGS RLLVVCDVALGKCVNLFKKDFSLTEAPPGYDSVHGVSETTSVPTDFQDDEFVVYKTNQVKMKYIVKFCTP GDQIKEFHPHENTEVEEQRAEPSSVPEAGDFQLPDIKPFTNIKAGLQDASANPVPLDSVHIKGRVIDFVA QVIVFQTYTNQSHVPIEAKYIFPLDDKAAVCGFEAFINGKHIVGEIKEKEEARQEYREAVSQGHGAYLMD QDTPDVFTVSVGNLPPRAKVLIKITYITELSIQSPVAIFFIPGTVAPWQQDKALNENLQDTVETIRIKEI GAEQSFSLAMSIEMPYMIEFISSDTHELRQKSTDCKAVVSTVEGSSLDSGGFSLHIGLRDAYLPRMWVEK HPEKESEACMLVFQPELADVLPDLRGKNEVIICLDCSSSMEGVTFTQAKQVALYALSLLGEEQKVNIMQF GTGYKELFSYPKCITDSKMATEFIMSAAPSMGNTDFWKVLRYLSLLYPSEGFRNILLISDGHLQSESLTL QLVKRNIQHTRVFTCAVGSTANRHILRTLSQCGAGVFEYFNSKSKHSWKKQIEAQMTRIRSPSCHSVSVK WQQLSRDAPEPLQAPAWVPSLFHNDRLLVYGFIPHCTQATLQAFIQEKEFCTMVSTTELQKTTGTMIHKL AARALIRDYEDGILHDDETNHEMKKNIMKSLIIELSKENSLITQFTSFVAVEKRDVNEIPFANVPNISEL VAKEDVDFLPYVSWQEKQPEASISQTEIDSSRLKHNKLSDGHGVLQPVSVSSEVNEKPSLLLAAKKRKIK TIKKCSLDISEDFEDRTAVAQSPATAQSLNFHLPLSVRPQLKAVEQQLHGNRLEPKQRGGFRKLLMAKKC RNVPDSLVSSAPAVTAEFSYLSACSSSSAFLSPLCDIPSSLPPHPLGGTHPPPPLPLPDGTHLPSPLFGS THPPPPLFGGTLIPPPSSLFGGTHLPPPPPLPGGTHIPPPPPIPGGTLIPPSSSLFGGTHLPPPPLLSAG THIPPPPLLSAGTHLPPPPLLPAGTHIPPPPPITGSTHPPPPSSLFGGTHLPPPPPLPGGTHIPPPPPIP GGTLIPSPSSLFGGTHLPPPPLLPAGTHIPPPPPITGSTHPPPPSSLFGGTHLPPPPPAGTQFSLSPIGF IPPKLGPPKLSHSHKLVGDTNIHDSEPPLLGFKDLCSRDMGFSCGTAFSGSFASSKDFDPGKFSQGPNNI SFSPKAPEMGVLHQSPFCSPPKPPSAPPLVTNVLCSEAPQSYFLNLQSAAVHQSPNNRVSEIIMESVESS LPSDYSSRDASSYLALEGAEDSLLGGSSFETDTDEAAAFIANDLLTSIETSSDEECAFCDEDQESPVPWA SLFALQTENGFWKLTPELGLILNLNVNALLTSLEEKGIRSLGTKGRERLLDLIATLLVLQFLYTKLEQEG MVAKSLIKMDDAFISRNIPWAFENIKKAREWARKTEGQYPSICQRLELGKDWESATKQLLGIQPQANTSL HRILYYSQG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001145978
ORF Size	5907 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001145978.2, NP_001139450.2
RefSeq Size	6391 bp
RefSeq ORF	5910 bp
Locus ID	328417
UniProt ID	E9PYK3
MW	216.1 kDa
Gene Summary	Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.[UniProtKB/Swiss-Prot Function]

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